Comment on ““Where does the fluorescing moiety reside in a carbon dot?” – Investigations based on fluorescence anisotropy decay and resonance energy transfer dynamics” by A. Das, D. Roy, C. K. De and P. K. Mandal, Phys. Chem. Chem. Phys., 2018, 20, 2251

In a recent paper published in Physical Chemistry Chemical Physics (Phys. Chem. Chem. Phys., 2018, 20, 2251–2259), Förster resonance energy transfer (FRET) between carbon dots and rhodamine 123 has been reported.

Orientational distribution of DPH in lipid membranes: a comparison of molecular dynamics calculations and experimental time-resolved anisotropy experiments

The behavior of the fluorescent probe diphenylhexatriene (DPH) in different lipid phases is investigated. The rotational autocorrelation functions are calculated in order to model the time-resolved fluorescence anisotropy decay. The role of the order parameters is discussed.

Reply to the ‘Comment on ““Where does the fluorescing moiety reside in a carbon dot?” – Investigations based on fluorescence anisotropy decay and resonance energy transfer dynamics”’ by H. C. Joshi, Phys. Chem. Chem. Phys., 2019, 21, DOI: 10.1039/c9cp00136k

Claims made in the Comment are perhaps incorrect and misleading. These claims have been negated with proper analytical reasoning.

“Where does the fluorescing moiety reside in a carbon dot?” – Investigations based on fluorescence anisotropy decay and resonance energy transfer dynamics

It has been shown recently that aggregated dyes are responsible for very high fluorescence in a carbon dot (CD). Location of the fluorescing unit in a carbon dot could be shown.