The possibility of improving the accuracy of measuring the average fluorescence lifetime by a modulation method

1974 ◽  
Vol 20 (4) ◽  
pp. 572-575
Author(s):  
A. V. Fal'tsman
Keyword(s):  
Fluorescence Lifetime ◽  
Modulation Method ◽  
Average Fluorescence

scholarly journals Changing Times: Fluorescence-lifetime Analysis of Amyloidogenic SF-IAPP Fusion Protein

2018 ◽  
Author(s):  
Olga I Antimonova ◽  
Dmitry V Lebedev ◽  
Yana A Zabrodskaya ◽  
Natalia A Grudinina ◽  
Michael M Shavlovsky ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Fluorescence Lifetime ◽  
Fluorescent Protein ◽  
Islet Amyloid ◽  
Cellular Models ◽  
Fluorescent Lifetime Imaging Microscopy ◽  
Lifetime Analysis ◽  
Changing Times ◽  
Green Fluorescent ◽  
Average Fluorescence

AbstractThe fluorescence lifetime of the superfolder green fluorescent protein (SF) and the SF protein fused with islet amyloid polypeptide (SF-IAPP) were studied in polyacrylamide gel. It was shown that the SF average fluorescence lifetime under these conditions slightly differs from that of the SF-IAPP monomer. SF-IAPP does not lose the ability to form amyloid-like fibrils; meanwhile, the average fluorescence lifetime of the fusion protein in fibrils is reduced. We propose the application of Fluorescent-lifetime Imaging Microscopy (FLIM) to the measurement of average fluorescence lifetimes of fusion proteins (amyloidogenic protein–SF) in the context of studies using cellular models of conformational diseases.

scholarly journals Nanosecond-order long–short fluorescence lifetime switchable encryption with enlarged coding capacity

Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Teng Luo ◽  
Yihua Zhao ◽  
Ting Zhou ◽  
Junle Qu
Keyword(s):  
Information Security ◽  
Fluorescence Lifetime ◽  
Weighted Average ◽  
Exponential Fitting ◽  
Double Exponential ◽  
Coding Capacity ◽  
Average Fluorescence

Abstract The turn-off fluorescent photoswitches for information encryption are constantly being developed. However, there are no reports about time-switchable (fluorescence lifetime-switchable) encryption to overcome the limitations of tunable encoding numbers in spectrally and temporally encoded libraries. Based on the double-exponential fitting of fluorescence lifetime, we propose, a fatigue-free and highly flexible switch between the amplitude-weighted average fluorescence lifetime (τm) and the intensity-weighted average fluorescence lifetime (τi), which will realize the supermultiplexed fluorescence lifetime switchable encryption. The potentially enormous library of different fluorescent lifetime combinations would facilitate the development of information security.

Multiplexed fluorescence lifetime imaging by concentration-dependent quenching

2018 ◽  
Vol 6 (13) ◽  
pp. 1912-1919 ◽  
Author(s):  
Teng Luo ◽  
Ting Zhou ◽  
Yihua Zhao ◽  
Liwei Liu ◽  
Junle Qu
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Multiplexed Imaging ◽  
Average Fluorescence

Working directly on an average fluorescence lifetime (τm) histogram for lifetime-based separation easily achieved multiplexed imaging of H&E stained sections.

Sign in / Sign up

Export Citation Format

Share Document