A quantitative study of peroxidase activity in unfixed tissue sections of the guinea-pig thyroid gland

1984 ◽  
Vol 16 (2) ◽  
pp. 111-122 ◽  
Author(s):  
Patricia A. Ealey ◽  
B. Henderson ◽  
N. Loveridge
Keyword(s):  
Thyroid Gland ◽  
Guinea Pig ◽  
Quantitative Study ◽  
Peroxidase Activity ◽  
Tissue Sections

scholarly journals DEMONSTRATION OF PEROXIDASE ACTIVITY IN TISSUE SECTIONS

1964 ◽  
Vol 12 (7) ◽  
pp. 538-544 ◽  
Author(s):  
MAX WACHSTEIN ◽  
ELIZABETH MEISEL
Keyword(s):  
Salivary Gland ◽  
Mast Cells ◽  
Guinea Pig ◽  
Peroxidase Activity ◽  
Striated Muscle ◽  
Acinar Cells ◽  
Hair Follicles ◽  
Tissue Sections ◽  
Pig Skin ◽  
Mammalian Tissues

By using an improved benzidine technique, peroxidase activity can be demonstrated in various locations in mammalian tissues. A relatively formalin resistant enzyme is found in hemoglobin and is also associated with mitochondria of striated muscle and heart. A somewhat less formalin resistant peroxidase occurs in the granules of myeloid and mast cells. A relatively formalin sensitive peroxidase is present in a number of additional locations, e.g. the acinar cells in thyroid and salivary gland, the medulla of the kidney, in hair follicles of the guinea pig skin and Kupffer cells of the liver.

Endogeneous peroxidase activity in the frog thyroid gland

Histochemie ◽  
1972 ◽  
Vol 32 (2) ◽  
pp. 99-105 ◽  
Author(s):  
Hisaka Nanba
Keyword(s):  
Thyroid Gland ◽  
Peroxidase Activity

scholarly journals Factors affecting the sensitivity and specificity of the cytochemical reaction for the anti-horseradish peroxidase antibody in lymph tissue sections.

1980 ◽  
Vol 28 (7) ◽  
pp. 645-652 ◽  
Author(s):  
W Straus
Keyword(s):  
Horseradish Peroxidase ◽  
Lymph Nodes ◽  
Sensitivity And Specificity ◽  
Peroxidase Activity ◽  
Additional Advantage ◽  
Tissue Sections ◽  
Endogenous Peroxidase ◽  
Cytochemical Reaction ◽  
Cold Acetone ◽  
Antibody Reaction

Factors which increase the sensitivity and specificity of the cytochemical reaction for the antibody to horseradish peroxidase (HRP) in precursors of plasma cells and in lymphocytes were studied in sections of popliteal lymph nodes of rats. The lymph nodes were removed 3-5 days after a secondary injection of HRP into the footpads and were fixed for 5 hr in a 4% cold formaldehyde solution (Straus W: Histochemistry 53:273, 1977). Brief postfixation of the frozen sections with cold acetone improved the retention of the antigen at the sites of the antibody in the precursor cells, and it improved the quality of fixation without appreciably weakening the antigen-binding capacity of the antibody. The cytochemical reaction for the anti-HRP antibody was intensified by staining with diaminobenzidine (DAB) and H2O2 at pH 5-6, or by staining at pH 7.4 in the presence of imidazole. Imidazole partially inhibited endogenous peroxidase activity. Pretreatment with phenylhydrazine prevented nonspecific background adsorption of HRP. Phenylhydrazine had the additional advantage of inhibiting most of the endogenous peroxidase activity (Straus W: J Histochem Cytochem 20:949, 1972). The intensity of the antibody reaction in the proplasma cells developing in the medullary cords varied greatly depending on the stage of maturation from lymphocytes and blast cells. Many lymphocytes in the cortex of the lymph node showed a strong perinuclear antibody reaction when the tissue sections were postfixed with cold acetone, and the peroxidase complexed to the antibody was visualized by staining with DAB and H2O2 at pH 5-6. The antibody reaction also occurred at the surgace of many lymphocytes when the tissue sections, postfixed with cold acetone, were stained with DAB and H2O2 at pH 7.4 in the presence of imidazole. Other lymphocytes showed a strong surface, perinuclear, and cytoplasmic antibody reaction after staining at pH 5-6 as well as after staining at pH 7.4, while yet other lymphocytes remained unstained.

A quantitative study of hearing in the guinea pig (Cavia cobaya).

1933 ◽  
Vol 15 (1) ◽  
pp. 59-73 ◽  
Author(s):  
G. P. Horton
Keyword(s):  
Guinea Pig ◽  
Quantitative Study

Peroxidase Activity in Thyroid Gland and Partial Purification of the Enzyme

1962 ◽  
Vol 52 (3) ◽  
pp. 180-189 ◽  
Author(s):  
TOICHIRO HOSOYA ◽  
YOICHI KONDO ◽  
UI NOBUO
Keyword(s):  
Thyroid Gland ◽  
Peroxidase Activity ◽  
Partial Purification

scholarly journals Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride

Blood ◽  
1965 ◽  
Vol 26 (2) ◽  
pp. 215-219 ◽  
Author(s):  
LEONARD S. KAPLOW ◽  
Charlotte Ladd
Keyword(s):  
Peroxidase Activity ◽  
Incubation Mixture ◽  
Tissue Sections ◽  
Blood Smears ◽  
Highly Sensitive ◽  
Frozen Tissue ◽  
Fresh Frozen

Abstract A method is described for demonstrating leukocyte peroxidase activity in which benzidine dihydrochloride is used as the indicator compound instead of the more commonly used but potentially more hazardous benzidine base. The method is highly sensitive and rapid and permits the use of fixed blood smears and organ imprints. The incubation mixture, which incorporates safranin as a counterstain, may be used over and over again, for as long as 6 months. The method is also applicable to fresh frozen tissue sections.

A quantitative study of the effects of different grades of polyvinyl alcohol on the activities of certain enzymes in unfixed tissue sections

1978 ◽  
Vol 10 (4) ◽  
pp. 453-463 ◽  
Author(s):  
B. Henderson ◽  
N. Loveridge ◽  
W. R. Robertson
Keyword(s):  
Polyvinyl Alcohol ◽  
Quantitative Study ◽  
Tissue Sections
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