Electron microscopic study of the ultra-thin sections of leprous peripheral nerves

1958 ◽  
Vol 18 (1-4) ◽  
pp. 411-423 ◽  
Author(s):  
Mitsugu Nishiura ◽  
Nobuo Harada ◽  
Tamotsu Imaeda
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Peripheral Nerves ◽  
Electron Microscopic ◽  
Thin Sections

scholarly journals Analytical electron microscopic study of mitochondrial inclusions in canine myocardial infarcts.

1976 ◽  
Vol 24 (3) ◽  
pp. 508-516 ◽  
Author(s):  
L M Buja ◽  
J H Dees ◽  
D F Harling ◽  
J T Willerson
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Mitochondrial Protein ◽  
Analytical Electron Microscopy ◽  
Muscle Cells ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Mitochondrial Inclusions ◽  
Analytical Electron

An analytical electron microscopic study, utilizing scanning transmission electron microscopy and energy-dispersive x-ray spectroscopy, was made of two types of mitochondrial inclusions identified in canine myocardial infarcts. The data were obtained from thin sections of tissues that were fixed in aldehyde, osmicated and embedded in epoxy resin. Calcium peaks of variable intensity were detected in inclusions which contained very electron-dense spicular material and which were localized to muscle cells at the peripheries of the infarcts. These findings indicate that the spicular inclusions represent early stages in the process of mitochondrial calcification in myocardial infarcts. In contrast, calcium or other trace elements were not detected in moderately electron-dense amorphous inclusions which were present in mitochondria of muscle cells throughout the infarcts. With the tissue preparative techniques employed, the possibility cannot be excluded that the amorphous inclusions contained calcium, either in small amounts or in a readily diffusable state, in vivo. The data, however, are in accord with the previously advanced hypothesis that the amorphous inclusions represent precipitates of denatured mitochondrial protein formed during the evolution of irreversible cellular injury. This study provides further evidence that analytical electron microscopy can yield important information regarding the nature of various inclusions occurring in normal and diseased tissues.

scholarly journals An Electron Microscopic Study of the Changes in Platelets during Viscous Metamorphosis

Blood ◽  
1962 ◽  
Vol 20 (5) ◽  
pp. 566-580 ◽  
Author(s):  
P. A. CASTALDI ◽  
B. G. FIRKIN ◽  
P. M. BLACKWELL ◽  
K. I. CLIFFORD
Keyword(s):  
Electron Microscope ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Light Microscope ◽  
Phase Contrast ◽  
Intact Cell ◽  
Complete Loss ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Sectioned Material

Abstract Viscous metamorphosis of platelets has been studied with the light microscope, and ultra-thin sections have been prepared at progressive stages for examination in the electron microscope. The phase contrast light microscope reveals rapid aggregation and distortion of platelets and gives the impression of their fusion into structureless aggregates during viscous metamorphosis. Sectioned material collected during viscous metamorphosis of platelets and examined in the electron microscope reveals a remarkable degree of retention of structure in a majority of the platelets. All become deficient in granules and devoid of vesicular spaces, but most retain intact cell membranes, and the structureless masses seen with the light microscope are found to consist of densely aggregated platelets. Fusion and complete loss of identity occurs in the minority. The retracted clot was found to contain densely aggregated, distorted and elongated platelets, empty of granules and intimately related to fibrin particles.

THE MICROMORPHOLOGY OF THE TUBERCULATE SPORES OF HISTOPLASMA CAPSULATUM

1960 ◽  
Vol 6 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Mercedes R. Edwards ◽  
Elizabeth L. Hazen ◽  
George A. Edwards
Keyword(s):  
Fine Structure ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Structural Changes ◽  
Histoplasma Capsulatum ◽  
Electron Microscopic ◽  
Cell Border ◽  
Thin Sections

An electron microscopic study of thin sections of the tuberculate spores of Histoplasma capsulatum is presented in which details of structure of the cytoplasm, cell border, and protruding tubercles as well as structural changes associated with growth and maturation of the spore are revealed. On the basis of the observations made on the fine structure, the mode of formation of the tubercles is considered.

An electron microscopic study of λ and λdg bacteriophage in thin sections

1965 ◽  
Vol 14 (2) ◽  
pp. 418-IN10 ◽  
Author(s):  
Donald J. Cummings ◽  
V.A. Chapman ◽  
S.S. DeLong
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
Thin Sections
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