Gene targeting using a mouse HPRT minigene/HPRT-deficient embryonic stem cell system: Inactivation of the mouseERCC-1 gene

1992 ◽  
Vol 18 (4) ◽  
pp. 325-336 ◽  
Author(s):  
Jim Selfridge ◽  
Angela M. Pow ◽  
Jim McWhir ◽  
Thomas M. Magin ◽  
David W. Melton
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Cell System ◽  
Stem Cell System

scholarly journals A novel self-organizing embryonic stem cell system reveals signaling logic underlying the patterning of human ectoderm

Development ◽  
2019 ◽  
Vol 146 (20) ◽  
pp. dev179093 ◽  
Author(s):  
George Britton ◽  
Idse Heemskerk ◽  
Rachel Hodge ◽  
Amina A. Qutub ◽  
Aryeh Warmflash
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cell System ◽  
Stem Cell System ◽  
Self Organizing

Live Monitoring of the Endoderm�like Cell Differentiation in the Murine Embryonic Stem Cell System

2004 ◽  
Vol 2004 (Fall) ◽  
Author(s):  
Irina Drobinskaya ◽  
Lubov Rochlina ◽  
Thomas Linn ◽  
Reinhard Bretzel ◽  
Eugen Kolossov ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cell System ◽  
Murine Embryonic Stem Cell ◽  
Stem Cell System

scholarly journals A homozygous Keap1-knockout human embryonic stem cell line generated using CRISPR/Cas9 mediates gene targeting

2017 ◽  
Vol 19 ◽  
pp. 52-54 ◽  
Author(s):  
So-Jung Kim ◽  
Omer Habib ◽  
Jin-Soo Kim ◽  
Hyo-Won Han ◽  
Soo Kyung Koo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Line ◽  
Gene Targeting ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem Cell Line ◽  
Embryonic Stem ◽  
Stem Cell Line

scholarly journals The search for Nkx2–5-regulated genes using purified embryonic stem cell-derived cardiomyocytes with Nkx2–5 gene targeting

2009 ◽  
Vol 390 (3) ◽  
pp. 821-826 ◽  
Author(s):  
Yasuhiro Nakashima ◽  
Koh Ono ◽  
Yoshinori Yoshida ◽  
Yoji Kojima ◽  
Toru Kita ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Gene Targeting ◽  
Embryonic Stem

scholarly journals Recycling selectable markers in mouse embryonic stem cells.

1996 ◽  
Vol 16 (4) ◽  
pp. 1851-1856 ◽  
Author(s):  
A Abuin ◽  
A Bradley
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Gene Targeting ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Free Cell ◽  
Selectable Markers ◽  
Dna Mismatch ◽  
Stem Cell Lines

As a result of gene targeting, selectable markers are usually permanently introduced into the mammalian genome. Multiple gene targeting events in the same cell line can therefore exhaust the pool of markers available and limit subsequent manipulations or genetic analysis. In this study, we describe the combined use of homologous and CRE-loxP-mediated recombination to generate mouse embryonic stem cell lines carrying up to four targeted mutations and devoid of exogenous selectable markers. A cassette that contains both positive and negative selectable markers flanked by loxP sites, rendering it excisable by the CRE protein, was constructed. Homologous recombination and positive selection were used to disrupt the Rep-3 locus, a gene homologous to members of the mutS family of DNA mismatch repair genes. CRE-loxP-mediated recombination and negative selection were then used to recover clones in which the cassette had been excised. The remaining allele of Rep-3 was then subjected to a second round of targeting and excision with the same construct to generate homozygous, marker-free cell lines. Subsequently, both alleles of mMsh2, another mutS homolog, were disrupted in the same fashion to obtain cell lines homozygous for targeted mutations at both the Rep-3 and mMsh2 loci and devoid of selectable markers. Thus, embryonic stem cell lines obtained in this fashion are suitable for further manipulation and analysis involving the use of selectable markers.

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