Effect of gammahydroxybutyric acid on serotonin synthesis, concentration and metabolism in the developing rat brain

Over 700 compounds were screened at 10−4 M concentration as inhibitors of the conversion of L-tryptophan-14C to serotonin-14C in crude rat brain homogenates. Most of the compounds had little or no inhibitory effect. Those with strong inhibitory properties were tested as inhibitors of 5-hydroxytryptophan decarboxylase and, if active on the decarboxylase, were assayed as tryptophan hydroxylase inhibitors. Except for a few oxidizing and complexing agents and for some substituted p-phenylenediamines, the compounds found to inhibit tryptophan hydroxylase by >50% belonged to the three types of inhibitors already known, i.e. catechols, phenylalanine and ring-substituted phenylalanines, and 6-substituted tryptophans. The numerous data in this screen make possible some comments as to the structural requirements for activity within each class. A comparison of the results on tryptophan hydroxylase with data on tyrosine hydroxylase inhibition in similar homogenates makes it clear that two separate, if somewhat similar, enzymes are involved.

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Synthesis and turnover of cerebroside sulfate of myelin in adult and developing rat brain

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)36813-9 ◽

1974 ◽

Vol 15 (2) ◽

pp. 114-123 ◽

Cited By ~ 1

Author(s):

Firoze B. Jungalwala

Keyword(s):

Rat Brain ◽

Cerebroside Sulfate ◽

Developing Rat

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Regulation of tubulin by triiodothyronine in hypothyroid rat brain

Bioscience Reports ◽

10.1007/bf01116995 ◽

1985 ◽

Vol 5 (8) ◽

pp. 643-648 ◽

Cited By ~ 2

Author(s):

Anjana Mazumder ◽

Kamal Das ◽

Pranab K. Sarkar

Keyword(s):

Protein Synthesis ◽

Rat Brain ◽

Brain Maturation ◽

Normal Brain ◽

Neonatal Brain ◽

Organ Cultures ◽

Postnatal Brain ◽

Enhanced Sensitivity ◽

Brain Exposure ◽

Developing Rat

The effect of T3 (triiodothyronine) on the induction of tubulin in hypothyroid developing rat brain has been examined using organ cultures of brains from late fetal, neonatal and postnatalrats. The neonatal brain displayed maximum sensitivity to T3. Hypothyroidism resulted in a 26% decline in the level of tubulin in the neonatal brain as opposed to a 5–15% decline in the fetal or postnatal brain. Exposure of the hypothyroi d neonatal brain to T3 for 2 h in culture led to a 61% rise in the level of tubulin in contrast to a 41% increase seen in the case of normal brain. Total protein synthesis was not significantly affected. The preferential decline of tubulin in the neonatal hypothyroid brain, its enhanced sensitivity to T3 compared to normal brain, and the coincidence of the period of sensitivity to that of brain maturation indicate that the regulation of the level of tubulin by T3 in the developing brain is a natural ontogenic phenomenon.

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