Results of chromosome analysis of rabies virus infected human diploid cells (WI-38)

1977 ◽  
Vol 54 (3) ◽  
pp. 255-257 ◽  
Author(s):  
M. Majer ◽  
Annelies Herrmann ◽  
R. Mauler
Keyword(s):  
Rabies Virus ◽  
Chromosome Analysis ◽  
Human Diploid Cells ◽  
Diploid Cells

Detection of antibodies to rabies virus by immunoelectron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 364-365
Author(s):  
R. K. Chaudhary ◽  
K. M. Charlton ◽  
M. T. Monette ◽  
A. E. Kelen
Keyword(s):  
Rabies Virus ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Standard Technique ◽  
Sucrose Density Gradient ◽  
Sucrose Density Gradient Centrifugation ◽  
Live Virus ◽  
Human Diploid Cells ◽  
Diploid Cells

Immunization of humans and domesticated animals at risk of contracting rabies is common practice. The mouse neutralization test (MNT) is still the standard technique used for detecting and measuring antibody to rabies virus in sera of vacinees. However, it suffers from problems of reproducibility associated with tests performed in vivo. It also has the disadvantage of being expensive, time-consuming and hazardous. Hence, there has been a continuous search for a simple, rapid and safe test. In recent years, methods based on haemagglutination, haemadsorption, plaque reduction, immunofluorescence and radioimmunoassay have been developed, but none of them has eliminated the hazard involved in the use of live virus.With emphasis on laboratory safety, attempts were made to use inactivated rabies virus for antibody assay by immunoelectronmicroscopy (IEM), in comparison with the MNT.Inactivated rabies virus grown in human diploid cells was supplied by Connaught Laboratories Limited (CLL). The virus was purified by sucrose density gradient centrifugation and used at a concentration to give 35-50 particles per grid square.

A comparison of the Pasteur and Pitman-Moore strains of rabies virus for the production of rabies vaccine in human diploid cells

1977 ◽  
Vol 5 (3) ◽  
pp. 249-256 ◽  
Author(s):  
M. Majer ◽  
Annelies Herrmann ◽  
J. Hilfenhaus ◽  
R. Mauler ◽  
H.-G. Lehmann ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Rabies Vaccine ◽  
Human Diploid Cells ◽  
Diploid Cells

scholarly journals Production of High-Titered Interferon in Cultures of Human Diploid Cells

1972 ◽  
Vol 2 (6) ◽  
pp. 476-484 ◽  
Author(s):  
E. A. Havell ◽  
J. Vilcek
Keyword(s):  
Human Diploid Cells ◽  
Diploid Cells

Growth of human diploid cells (strain MRC-5) in defined medium; replacement of serum by a fraction of serum ultrafiltrate

1979 ◽  
Vol 35 (1) ◽  
pp. 381-392
Author(s):  
K. Lambert ◽  
S.J. Pirt
Keyword(s):  
Amino Acids ◽  
Active Material ◽  
Maximum Yield ◽  
Calf Serum ◽  
Defined Medium ◽  
Cell Yield ◽  
Maximum Cell ◽  
Macromolecular Component ◽  
Human Diploid Cells ◽  
Diploid Cells

A calf serum ultrafiltrate fraction permitted growth for at least 3.5 generations, including one subculture, of MRC-5 cells in defined medium in the absence of whole serum. The active material has a molecular weight of 10 000 Daltons or less. This suggests that there may be no requirement for a large macromolecular component of serum. The ultrafiltrate was assayed by maximum cell yield from a serum-limited inoculum in a defined medium containing non-limiting amounts of vitamins, amino acids, glucose, a 68-component supplement, iron and methylcellulose. The levels of vitamins, amino acids and glucose were based on quantitative measurements of uptake and the levels of the other components by minimum amount required for maximum yield in defined medium without ultrafiltrate or serum. With excess ultrafiltrate maximum cell yield was limited by the defined part of the medium, probably the supplement. The cell doubling time in defined medium with ultrafiltrate fractions was 70 h compared with 27 h in the medium with serum. Excess ultrafiltrate did not inhibit growth. The lowered growth rate is attributed to a nutritional deficiency in the supplement.

Efficient transfer of cloned DNA into human diploid cells: protoplast fusion in suspension

1984 ◽  
Vol 4 (11) ◽  
pp. 2549-2552
Author(s):  
P Litzkas ◽  
K K Jha ◽  
H L Ozer
Keyword(s):  
Simian Virus 40 ◽  
Cell Types ◽  
Simian Virus ◽  
Selective Medium ◽  
T Antigen ◽  
Human Diploid Fibroblasts ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Human Diploid Cells ◽  
Diploid Cells

A method for fusion of protoplasts bearing amplified plasmids and human diploid fibroblasts or other cell types in suspension is described. Transient expression of plasmid-encoded proteins occurs in up to 50% of the human cells, as demonstrated for simian virus 40 T antigen by immunofluorescence and the Escherichia coli xanthine-guanine phosphoribosyl transferase by autoradiography. In contrast, frequencies of stable transformants were similar to those obtained by the CaPO4 coprecipitation technique. However, experiments with both methods involving the recombinant pRSVneo (in which the Rous sarcoma virus long terminal repeat regulates expression of the antibiotic-inactivating aminoglycoside phosphotransferase) revealed a much higher frequency of colonies in G418 selective medium with constructions in which the early region of simian virus 40 DNA was present as well. We propose a role for the simian virus 40 T antigen in enhancing stable transformation in this system.

scholarly journals ASYNCHRONY OF DNA SYNTHESIS IN CHROMOSOMES OF HUMAN DIPLOID CELLS

1963 ◽  
Vol 16 (1) ◽  
pp. 202-209 ◽  
Author(s):  
P. S. Moorhead ◽  
Vittorio Defendi
Keyword(s):  
Dna Synthesis ◽  
Human Diploid Cells ◽  
Diploid Cells

Phosphorylated Histone H2AX Foci Persist on Rejoined Mitotic Chromosomes in Normal Human Diploid Cells Exposed to Ionizing Radiation

2006 ◽  
Vol 165 (3) ◽  
pp. 269-276 ◽  
Author(s):  
Masatoshi Suzuki ◽  
Keiji Suzuki ◽  
Seiji Kodama ◽  
Masami Watanabe
Keyword(s):  
Ionizing Radiation ◽  
Mitotic Chromosomes ◽  
Histone H2ax ◽  
Normal Human ◽  
Human Diploid Cells ◽  
Diploid Cells
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