Role of steroidal glycoalkaloid ?-tomatine in host-plant resistance of tomato to colorado potato beetle

The colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), is the leading insect pest of potato (Solanum tuberosum L.) in northern latitudes. Host plant resistance has the potential use in an integrated pest management program for control of colorado potato beetle. During the 1998 and 1999 seasons, field studies were conducted to compare natural (leptine glycoalkaloids and glandular trichomes), engineered (Bt-cry3A and Bt-cry5 transgenic potato lines), and combined (Bt-cry5+glandular trichomes) plant resistance mechanisms of potato for control of colorado potato beetle. Nine different potato clones representing five different host plant resistance mechanisms were evaluated under natural colorado potato beetle infestation at the Montcalm Research Farm in Entrican, Michigan. The Bt-cry3A transgenic lines, the high leptine line (USDA8380-1), and the high foliar glycoalkaloid line (ND5873-15) were most effective for controlling defoliation by colorado potato beetle adults and larvae. The Bt-cry5 line (SPc5-G2) was not as effective as the Bt-cry3A transgenic lines ('Russet Burbank Newleaf,' RBN15, and YGc3.1). The glandular trichome (NYL235-4) and Bt-cry5+glandular trichome lines proved to be ineffective. Significant rank correlations for the potato lines between the two years were observed for egg masses, second and third instar, and fourth instar seasonal cumulative mean number of individuals per plant, and defoliation. Egg mass and first instar seasonal cumulative mean number of individuals per plant were not strong indicators of host plant resistance in contrast to second and third instars or adults. Based on these results, the Bt-cry3A transgenic lines, the high leptine line, and the high total glycoalkaloid line are effective host plant resistance mechanisms for control of colorado potato beetle.

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Combining Natural and Engineered Host Plant Resistance Mechanisms in Potato for Colorado Potato Beetle: Choice and No-choice Field Studies

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.130.6.857 ◽

2005 ◽

Vol 130 (6) ◽

pp. 857-864 ◽

Cited By ~ 3

Author(s):

Joseph J. Coombs ◽

David S. Douches ◽

Susannah G. Cooper ◽

Edward J. Grafius ◽

Walter L. Pett ◽

...

Keyword(s):

Host Plant ◽

Plant Resistance ◽

Colorado Potato Beetle ◽

Host Plant Resistance ◽

Glandular Trichomes ◽

Field Studies ◽

Resistance Mechanisms ◽

Field Cage ◽

Potato Beetle ◽

Transgenic Lines

Colorado potato beetle (Leptinotarsa decemlineata Say) is the leading insect pest of potato (Solanum tuberosum L.) in northern latitudes. Host plant resistance is an important tool in an integrated pest management program for controlling insect pests. Field studies were conducted to compare natural host plant resistance mechanisms (glandular trichomes and Solanum chacoense Bitter-derived resistance), engineered [Bacillus thuringiensis (Bt) Berliner Bt-cry3A], and combined (glandular trichomes + Bt-cry3A and S. chacoense-derived resistance + Bt-cry3A transgenic potato lines) sources of resistance for control of colorado potato beetle. Six different potato clones representing five different host plant resistance mechanisms were evaluated for 2 years in a field situation under natural colorado potato beetle pressure in Michigan and New York, and in a no-choice field cage study in Michigan. In the field studies, the S. chacoense-derived resistance line, Bt-cry3A transgenic, and combined resistance lines were effective in controlling defoliation by colorado potato beetle adults and larvae. Effectively no feeding was observed in the Bt-cry3A transgenic lines. The glandular trichome line suffered less defoliation than the susceptible control, but had greater defoliation than the Bt-cry3A transgenic lines and the S. chacoense-derived resistance line. In the no-choice cage study, the Bt-cry3A transgenic lines and the combined resistance lines were effective in controlling feeding by colorado potato beetle adults and larvae with no defoliation observed. The S. chacoense-derived resistance line and the glandular trichome line suffered less defoliation than the susceptible control. Based on the results of the field trials and no-choice field cage studies, these host plant resistance mechanisms could be used to develop potato varieties for use in a resistance management program for control of colorado potato beetle.

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Insecticidal Activity of Avidin Combined with Genetically Engineered and Traditional Host Plant Resistance Against Colorado Potato Beetle (Coleoptera: Chrysomelidae) Larvae

Journal of Economic Entomology ◽

10.1093/jee/99.2.527 ◽

2006 ◽

Vol 99 (2) ◽

pp. 527-536 ◽

Cited By ~ 5

Author(s):

Susannah G. Cooper ◽

David S. Douches ◽

Edward J. Grafius

Keyword(s):

Host Plant ◽

Insecticidal Activity ◽

Plant Resistance ◽

Colorado Potato Beetle ◽

Host Plant Resistance ◽

Genetically Engineered ◽

Potato Beetle

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Comparing Host Plant Resistance, Engineered Resistance, and Insecticide Treatment for Control of Colorado Potato Beetle and Potato Leafhopper in Potatoes

International Journal of Agronomy ◽

10.1155/2011/390409 ◽

2011 ◽

Vol 2011 ◽

pp. 1-4 ◽

Cited By ~ 1

Author(s):

Gerald M. Ghidiu ◽

David S. Douches ◽

Kimberly J. Felcher ◽

Joseph J. Coombs

Keyword(s):

Host Plant ◽

Plant Resistance ◽

Colorado Potato Beetle ◽

Host Plant Resistance ◽

Glandular Trichomes ◽

Potato Leafhopper ◽

Management Options ◽

Potato Leaf ◽

Potato Beetle ◽

Engineered Resistance

The Colorado potato beetle,Leptinotarsa decemlineata(Say) Order Coleoptera and the potato leafhopper,Empoasca fabae(Harris) Order Homoptera, are the major insect pests of potato in eastern North America. In two years of field trials, we compared the effectiveness of three pest management options for the control of Colorado potato beetle and potato leafhopper: natural host plant resistance (glandular trichomes), engineered resistance (Bacillus thuringiensis[Bt] Berlinercry3Agene) and a susceptible potato cultivar (Superior) with an at-planting application of the insecticide thiamethoxam. Similar and acceptable control of the Colorado potato beetle larvae was obtained with theBt-cry3Alines and the thiamethoxam treated “Superior” variety. The glandular trichome cultivar had significantly less Colorado potato beetle damage than did the untreated “Superior” in 2004, although damage was significantly greater than in theBt-cry3Alines and the insecticide-treated potatoes for both years, and was the only treatment that consistently had very little potato leafhopper damage. These data demonstrate that although each type of host plant resistance mechanism (Bt-cry3Aor glandular trichomes) was as effective as the chemical control against one of the insects, neither provides adequate resistance to both Colorado potato beetle and potato leaf hopper.

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Effectiveness of Natural and Engineered Host Plant Resistance in Potato to the Colorado Potato Beetle

HortScience ◽

10.21273/hortsci.36.5.967 ◽

2001 ◽

Vol 36 (5) ◽

pp. 967-970 ◽

Cited By ~ 8

Author(s):

D.S. Douches ◽

T.J. Kisha ◽

J.J. Coombs ◽

W. Li ◽

W.L. Pett ◽

...

Keyword(s):

Host Plant ◽

Plant Resistance ◽

Colorado Potato Beetle ◽

Host Plant Resistance ◽

Insect Pest ◽

Glandular Trichomes ◽

Glandular Trichome ◽

Larval Feeding ◽

Potato Beetle ◽

Transgenic Lines

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is the most serious insect pest of potatoes throughout the eastern and north central United States. Host plant resistance to the Colorado potato beetle has been identified in wild Solanum species and Bt-transgenic potato lines. Detached-leaf bioassays (72 h) were conducted on insecticide-resistant, first instar Colorado potato beetles to study the effectiveness of individual and combined host plant resistance traits in potato. Potato lines tested include non-transgenic cultivars (`Russet Burbank', `Lemhi Russet', and `Spunta'), a line with glandular trichomes (NYL235-4), a line with high foliar leptines (USDA8380-1), and transgenic lines expressing either codon-modified Bt-cry3A or Bt-cry5 (Bt-cry1Ia1). Bt-cry3A transgenic lines, foliar leptine line, and foliar leptine lines with Bt-cry5 had reduced feeding compared to non-transgenic cultivars. Glandular trichome lines and glandular trichome lines with Bt-cry5 did not reduce feeding in this no-choice feeding study. Some Bt-cry5 transgenic lines, using either the constitutive promoters CaMV35s or (ocs)3mas (Gelvin super promoter), were moderately effective in reducing larval feeding. Feeding on Bt-cry5 transgenic lines with the tuber-specific patatin promoter was not significantly different than or greater than feeding on the susceptible cultivars. Mortality of first instars was highest when fed on the Bt-cry3A lines (68% to 70%) and intermediate (38%) on the Bt-cry5 `Spunta' line SPG3 where the gus reporter gene was not included in the gene construct. Host plant resistance from foliar leptines is a candidate mechanism to pyramid with either Bt-cry3A or Bt-cry5 expression in potato foliage against Colorado potato beetle. Without multiple sources of host plant resistance, long-term sustainability is questionable for a highly adaptable insect like the Colorado potato beetle.

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Hypersensitivity and Egg Drop: A Novel Mechanism of Host Plant Resistance to Colorado Potato Beetle (Coleoptera: Chrysomelidae)

Journal of Economic Entomology ◽

10.1093/jee/90.2.652 ◽

1997 ◽

Vol 90 (2) ◽

pp. 652-657 ◽

Cited By ~ 55

Author(s):

Nikolay F. Balbyshev ◽

James H. Lorenzen

Keyword(s):

Host Plant ◽

Plant Resistance ◽

Colorado Potato Beetle ◽

Host Plant Resistance ◽

Potato Beetle

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Mechanisms of Soybean Host-Plant Resistance Against Megacopta cribraria (Hemiptera: Plataspidae)

Environmental Entomology ◽

10.1093/ee/nvaa075 ◽

2020 ◽

Vol 49 (4) ◽

pp. 876-885

Author(s):

S Lahiri ◽

D D Reisig ◽

Lisa L Dean ◽

F P F Reay-Jones ◽

J K Greene ◽

...

Keyword(s):

Host Plant ◽

Plant Resistance ◽

Host Plant Resistance ◽

Hexadecenoic Acid ◽

Mechanisms Of Resistance ◽

Useful Knowledge ◽

Plant Volatile ◽

Volatile Organic ◽

Positive Correlations

Abstract A number of soybean varieties traditionally bred for resistance to various soybean arthropod pests have been identified as resistant to Megacopta cribraria (F.) (Hemiptera: Plataspidae). However, the mechanisms of host-plant resistance (HPR) in this system are not understood. The goal of this study was to identify the mechanisms of resistance by examining the role of plant volatile organic compounds (VOCs) and free amino acids (FAAs) among 16 soybean varieties. Choice and no-choice cage experiments identified several soybean varieties that demonstrated antixenosis as well as antibiosis. However, resistance varied over time in certain soybean varieties, such as N02-7002 and PI567352B. Mean nymph number from choice experiments had positive correlations with the FAAs asparagine, tryptophan, alanine, phenylanaline, and serine; negative correlation with leucine and threonine. Four plant volatiles, hexanal, 2-pentylfuran, beta-cyclocitral, and cis-9-hexadecenal, were positively correlated with subsequent nymph development, whereas n-hexadecenoic acid was negatively correlated with nymph number only, in adult choice cage experiments. This study contributes to understanding the mechanisms of HPR through associations with plant VOCs and FAAs in relation to M. cribraria development and provides useful knowledge for developing soybean varieties for M. cribraria management.

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