Prothrombin determination in plasma and in liver biopsies in chronic liver diseases

Abstract Background: Chronic liver diseases can progress to cirrhosis and to hepatocellular carcinoma. Timely and unequivocal recognition of the neoplastic evolution of cirrhosis is critical. To this aim, we used a noncompetitive reverse transcription-PCR procedure to analyze aldolase A mRNA in liver tissue from patients with chronic liver diseases at different stages. Methods: We studied 12 patients with hepatocellular carcinoma, 19 patients affected by chronic hepatitis C or cirrhosis, and 7 healthy controls. Aldolase A mRNA was reverse-transcribed to cDNA, which was then amplified by PCR. The amplified segments were “read” with a novel dot-blot procedure. A calibrator with the same sequence, synthesized in vitro using a T7 phage promoter, was processed at scalar dilutions in parallel to the target samples to generate a calibration curve and so quantify the target mRNA (detection limit, 0.03 amol; linearity spanning five orders of magnitude). Results: Aldolase A mRNA was ∼10-fold higher in liver biopsies from patients with hepatocellular carcinoma vs patients with chronic hepatitis C or cirrhosis, and healthy individuals. Furthermore, aldolase A mRNA concentrations were 1.2- to 21.3-fold higher in 12 liver biopsies compared with the paired surrounding cirrhotic tissue. Conclusions: The quantitative analysis of liver tissue aldolase A mRNA differentiates between nonneoplastic chronic liver diseases and hepatocellular carcinoma, which suggests that it has diagnostic potential.

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Multispectral Imaging Differentiates Unique Macrophage Profiles in Patients with Distinct Chronic Liver Diseases

10.1101/794610 ◽

2019 ◽

Author(s):

Omar A. Saldarriaga ◽

Adam L. Booth ◽

Benjamin Freiberg ◽

Jared Burks ◽

Santhoshi Krishnan ◽

...

Keyword(s):

Liver Diseases ◽

Multispectral Imaging ◽

Treatment Strategies ◽

Spectral Imaging ◽

Spectral Unmixing ◽

Chronic Liver ◽

Chronic Liver Diseases ◽

Liver Biopsies ◽

Anti Inflammatory

ABSTRACTIntrahepatic macrophages influence the composition of the microenvironment, host immune response to liver injury, and development of fibrosis. Compared to stellate cells, the role of intrahepatic macrophages in the development of fibrosis remains ill defined. Multispectral imaging allows detection of multiple markers in situ in human formalin-fixed, paraffin-embedded tissue. This cutting-edge technology is ideal for analyzing human liver tissues since it allows spectral unmixing of fluorophore signals, subtraction of auto-fluorescence, and preserves architecture and the in vivo hepatic milieu. We analyzed resident Kupffer cells (CD68+), monocyte-derived macrophages (Mac387+), pro-fibrogenic macrophages (CD163+), and co-expression of pro-inflammatory (CD14) and anti-inflammatory (CD16) markers in liver biopsies from patients with hepatitis C virus (HCV) and different stages of fibrosis. Liver biopsies with advanced fibrosis showed increased accumulation of CD163+, MAC387+ and CD68+ macrophages in the portal tracts when compared to those with minimal fibrosis. Imaging software generated t-distributed stochastic neighbor embedding (t-SNE) plots and phenotype matrices that facilitated comparison of macrophage profiles. These included monocyte-derived (CD68+/Mac387+) and pro-fibrotic/anti-inflammatory (CD163+/CD16+) phenotypes. We established that the utility of this platform could be extended to liver biopsies from patients with other chronic liver diseases including nonalcoholic steatohepatitis and autoimmune hepatitis. Each disease exhibited a unique profile after spectral imaging analysis and this platform holds the potential to identify patients predisposed to progressive liver disease based on the macrophage composition. In summary, spectral imaging is a powerful tool that enables analysis of macrophage profiles in different types of chronic liver diseases and has potential to change the manner in which we evaluate liver biopsies leading to more personalized treatment strategies.

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Detection of nuclear oncoproteins in liver biopsies from patients with HCV-associated chronic liver diseases: Clinical relationship and prognostic significance

Hepatology ◽

10.1016/0270-9139(94)90690-4 ◽

1994 ◽

Vol 19 (4) ◽

pp. I131

Author(s):

G Tappero

Keyword(s):

Liver Diseases ◽

Prognostic Significance ◽

Chronic Liver ◽

Chronic Liver Diseases ◽

Clinical Relationship ◽

Liver Biopsies

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Activity of procollagen-prolyl-hydroxylase and N-acetyl-?-glucosaminidase in liver biopsies from patients with chronic liver diseases

Klinische Wochenschrift ◽

10.1007/bf01491138 ◽

1979 ◽

Vol 57 (11) ◽

pp. 587-588 ◽

Cited By ~ 6

Author(s):

G. Pott ◽

G. Eberhardt ◽

U. Gerlach

Keyword(s):

Liver Diseases ◽

Prolyl Hydroxylase ◽

Chronic Liver ◽

Chronic Liver Diseases ◽

Liver Biopsies

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Transjugular liver biopsy: histological diagnosis success comparing the trucut to the modified aspiration Ross needle

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032003000200004 ◽

2003 ◽

Vol 40 (2) ◽

pp. 80-84 ◽

Cited By ~ 9

Author(s):

Antonio Carlos Maciel ◽

Edson Marchiori ◽

Sérgio Gabriel Silva de Barros ◽

Carlos Thadeu S. Cerski ◽

Dorvaldo Paulo Tarasconi ◽

...

Keyword(s):

Liver Biopsy ◽

Liver Diseases ◽

Chronic Liver ◽

Histological Diagnosis ◽

Chronic Liver Diseases ◽

Porto Alegre ◽

Massive Ascites ◽

Transjugular Liver Biopsy ◽

Liver Biopsies ◽

Trucut Needle

BACKGROUND: Transjugular liver biopsy is an alternative procedure for patients who present contraindications to standard percutaneous procedure. AIM: To compare the rate of histological diagnosis obtained on transjugular liver biopsy with an automated trucut needle and with a modified Ross needle. PATIENTS / METHOD: Eighty-five patients with suspicion of chronic liver diseases and presenting contraindications for percutaneous liver biopsy (coagulopathy, massive ascites, morbid obesity, or chronic renal problems) were submitted to 89 transjugular liver biopsies between March 1994 and April 2001 at ''Hospital São José, Irmandade da Santa Casa de Misercórdia'', Porto Alegre, RS, Brazil. Thirty-five patients underwent 36 biopsies with an automated trucut needle, and 50 patients underwent 53 biopsies with a modified Ross needle. RESULTS: Histological diagnosis was reached in 32/35 subjects submitted to transjugular liver biopsy with the trucut needle (91%) and in 35/50 (70%) submitted to biopsy with the modified Ross needle. Specimens obtained with the trucut needle were significantly larger and less fragmented than those obtained with the Ross needle. CONCLUSION: Transjugular liver biopsy with the automated trucut needle allowed a higher rate of histological diagnosis when compared to the modified Ross needle in patients with suspicion of chronic liver diseases.

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