Temperature dependence of single channel currents and the peptide libration mechanism for ion transport through the gramicidin A transmembrane channel

1984 ◽  
Vol 81 (3) ◽  
pp. 205-217 ◽  
Author(s):  
D. W. Urry ◽  
S. Alonso-Romanowski ◽  
C. M. Venkatachalam ◽  
R. J. Bradley ◽  
R. D. Harris
Keyword(s):  
Ion Transport ◽  
Temperature Dependence ◽  
Single Channel ◽  
Gramicidin A ◽  
Transmembrane Channel ◽  
Single Channel Currents ◽  
Channel Currents

A Calcium-Activated Nonspecific Cation Channel from Olfactory Receptor Neurones of the Silkmoth Antheraea Polyphemus

1991 ◽  
Vol 161 (1) ◽  
pp. 455-468
Author(s):  
F. ZUFALL ◽  
H. HATT ◽  
T. A. KEIL
Keyword(s):  
Olfactory Receptor ◽  
Single Channel ◽  
Membrane Vesicles ◽  
Cation Channel ◽  
Voltage Response ◽  
Antheraea Polyphemus ◽  
Cell Excitability ◽  
Olfactory Receptor Neurones ◽  
Single Channel Currents ◽  
Channel Currents

Single-channel patch-clamp techniques were used to identify and characterize a Ca2+-activated nonspecific cation channel (CAN channel) on insect olfactory receptor neurones (ORNs) from antennae of male Antheraea polyphemus. The CAN channel was found both in acutely isolated ORNs from developing pupae and in membrane vesicles from mature ORNs that presumably originated from inner dendritic segments. Amplitude histograms of the CAN single-channel currents presented well-defined peaks corresponding to at least four channel substates each having a conductance of about 16 pS. Simultaneous gating of the substates was achieved by intracellular Ca2+ with an EC50 value of about 80 nmoll−1. Activity of the CAN channel could be blocked by application of amiloride (IC50 <100nmoll−1). Moreover, in the presence of 1μmoll−1 Ca2+, opening of the CAN channel was totally suppressed by 10 μmoll−1 cyclic GMP, whereas ATP (1 mmol l−1) was without effect. We suggest that the CAN channel plays a specific role in modulation of cell excitability and in shaping the voltage response of ORNs.

SINGLE CHANNEL CURRENTS IN RETINAL CELLS

1986 ◽  
pp. 209-212
Author(s):  
Davide LOVISOLO ◽  
Renzo LEVI ◽  
Dario CANTINO ◽  
Elena STROBBIA
Keyword(s):  
Single Channel ◽  
Retinal Cells ◽  
Single Channel Currents ◽  
Channel Currents
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