scholarly journals An ultrastructural study of isolated rat skeletal muscle mitochondria in various metabolic states

1970 ◽  
Vol 2 (1) ◽  
pp. 71-84 ◽  
Author(s):  
Jerry M. Kuner ◽  
Robert E. Beyer
Keyword(s):  
Skeletal Muscle ◽  
Ultrastructural Study ◽  
Rat Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Metabolic States ◽  
Isolated Rat

Phosphocreatine Synthesis by Isolated Rat Skeletal Muscle Mitochondria Is Not Dependent upon External ADP: A 31P NMR Study

1996 ◽  
Vol 225 (3) ◽  
pp. 819-825 ◽  
Author(s):  
Florence Kernec ◽  
Nathalie Le Tallec ◽  
Lydie Nadal ◽  
Jean-Marc Bégué ◽  
Elisabeth Le Rumeur
Keyword(s):  
Skeletal Muscle ◽  
31P Nmr ◽  
Rat Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Nmr Study ◽  
Isolated Rat

Proton leak induced by reactive oxygen species produced during in vitro anoxia/reoxygenation in rat skeletal muscle mitochondria

2006 ◽  
Vol 38 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Rachel Navet ◽  
Ange Mouithys-Mickalad ◽  
Pierre Douette ◽  
Claudine M. Sluse-Goffart ◽  
Wieslawa Jarmuszkiewicz ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Skeletal Muscle ◽  
Reactive Oxygen ◽  
Proton Leak ◽  
Oxygen Species ◽  
Rat Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria

scholarly journals Production of superoxide/H2O2 by dihydroorotate dehydrogenase in rat skeletal muscle mitochondria

2014 ◽  
Vol 72 ◽  
pp. 149-155 ◽  
Author(s):  
Martin Hey-Mogensen ◽  
Renata L.S. Goncalves ◽  
Adam L. Orr ◽  
Martin D. Brand
Keyword(s):  
Skeletal Muscle ◽  
Dihydroorotate Dehydrogenase ◽  
Rat Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria

Characterization of superoxide production sites in isolated rat brain and skeletal muscle mitochondria

2005 ◽  
Vol 59 (4) ◽  
pp. 163-168 ◽  
Author(s):  
Alexey P. Kudin ◽  
Grazyna Debska-Vielhaber ◽  
Wolfram S. Kunz
Keyword(s):  
Skeletal Muscle ◽  
Rat Brain ◽  
Superoxide Production ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Isolated Rat

Artifactual uncoupling by uncoupling protein 3 in yeast mitochondria at the concentrations found in mouse and rat skeletal-muscle mitochondria

2001 ◽  
Vol 361 (1) ◽  
pp. 49-56 ◽  
Author(s):  
James A. HARPER ◽  
Jeff A. STUART ◽  
Mika B. JEKABSONS ◽  
Damien ROUSSEL ◽  
Kevin M. BRINDLE ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Uncoupling Protein ◽  
Mitochondrial Protein ◽  
Yeast Mitochondria ◽  
Rat Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Uncoupling Protein 3 ◽  
Brown Adipose

Western blots detected uncoupling protein 3 (UCP3) in skeletal-muscle mitochondria from wild-type but not UCP3 knock-out mice. Calibration with purified recombinant UCP3 showed that mouse and rat skeletal muscle contained 0.14μg of UCP3/mg of mitochondrial protein. This very low UCP3 content is 200–700-fold less than the concentration of UCP1 in brown-adipose-tissue mitochondria from warm-adapted hamster (24–84μg of UCP1/mg of mitochondrial protein). UCP3 was present in brown-adipose-tissue mitochondria from warm-adapted rats but was undetectable in rat heart mitochondria. We expressed human UCP3 in yeast mitochondria at levels similar to, double and 7-fold those found in rodent skeletal-muscle mitochondria. Yeast mitochondria containing UCP3 were more uncoupled than empty-vector controls, particularly at concentrations that were 7-fold physiological. However, uncoupling by UCP3 was not stimulated by the known activators palmitate and superoxide; neither were they inhibited by GDP, suggesting that the observed uncoupling was a property of non-native protein. As a control, UCP1 was expressed in yeast mitochondria at similar concentrations to that of UCP3 and at up to 50% of the physiological level of UCP1. Low levels of UCP1 gave palmitate-dependent and GDP-sensitive proton conductance but higher levels of UCP1 caused an additional GDP-insensitive uncoupling artifact. We conclude that the uncoupling of yeast mitochondria by high levels of UCP3 expression is entirely an artifact and provides no evidence for any native uncoupling activity of the protein.

Glutaminase activity in rat skeletal muscle mitochondria

1978 ◽  
Vol 34 (2) ◽  
pp. 165-166 ◽  
Author(s):  
J. Świerczyński ◽  
W. Makarewicz
Keyword(s):  
Skeletal Muscle ◽  
Rat Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Glutaminase Activity
Sign in / Sign up

Export Citation Format

Share Document