l-Carnitine Stimulation of Mitochondrial Oxidative Phosphorylation Rate in Isolated Rat Skeletal Muscle Mitochondria

1997 ◽  
Vol 117 (1) ◽  
pp. 141-145 ◽  
Author(s):  
Phanélie Berthon ◽  
Marieke Van Der Veer ◽  
Christian Denis ◽  
Damien Freyssenet
Keyword(s):  
Skeletal Muscle ◽  
Oxidative Phosphorylation ◽  
Mitochondrial Oxidative Phosphorylation ◽  
Rat Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Phosphorylation Rate ◽  
Isolated Rat ◽  
Stimulation Of

A magnesium-responsive defect of respiration and oxidative phosphorylation in skeletal muscle mitochondria of dystrophic hamsters

1970 ◽  
Vol 48 (12) ◽  
pp. 1332-1338 ◽  
Author(s):  
K. Wrogemann ◽  
M. C. Blanchaer ◽  
B. E. Jacobson
Keyword(s):  
Skeletal Muscle ◽  
Oxidative Phosphorylation ◽  
Respiratory Rate ◽  
Test System ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Muscle Necrosis ◽  
Presence And Absence ◽  
Stimulation Of

Skeletal muscle mitochondria were isolated in the presence and absence of the proteinase Nagarse from dystrophic hamsters of the BIO 14.6 strain, aged 45–196 days, and from normal hamsters. Mitochondria from the dystrophic animals prepared by glass-on-glass homogenization without Nagarse in 0.25 M sucrose – 1 mM EDTA, pH 7.4, did not differ from normal in their respiratory rate or capacity for oxidative phosphorylation. However, these functions were subnormal in mitochondria isolated with Nagarse from the same animals, both in the presence and absence of albumin. Respiration measured with an O2 electrode was reduced by 50–70% and the stimulation of O2 uptake normally seen after ADP addition was minimal or absent. This was most marked in mitochondria from young hamsters about 65 days old with muscle necrosis. The defect was ameliorated by addition to the Polarographie test system of an ATP trap or of Mg2+, one of the trap constituents. This ion, when added to the defective mitochondria prior to ADP and substrate, restored respiration and oxidative phosphorylation to values that did not differ significantly from those found with skeletal muscle mitochondria of normal hamsters.

Phosphocreatine Synthesis by Isolated Rat Skeletal Muscle Mitochondria Is Not Dependent upon External ADP: A 31P NMR Study

1996 ◽  
Vol 225 (3) ◽  
pp. 819-825 ◽  
Author(s):  
Florence Kernec ◽  
Nathalie Le Tallec ◽  
Lydie Nadal ◽  
Jean-Marc Bégué ◽  
Elisabeth Le Rumeur
Keyword(s):  
Skeletal Muscle ◽  
31P Nmr ◽  
Rat Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Nmr Study ◽  
Isolated Rat

Characterization of frog muscle mitochondria

1978 ◽  
Vol 234 (1) ◽  
pp. C1-C6 ◽  
Author(s):  
C. Skoog ◽  
U. Kromer ◽  
R. W. Mitchell ◽  
J. Hoogstraten ◽  
N. L. Stephens
Keyword(s):  
Skeletal Muscle ◽  
Oxidative Phosphorylation ◽  
Oxidase Activity ◽  
Nadh Oxidase ◽  
Mitochondrial Protein ◽  
Frog Skeletal Muscle ◽  
Muscle Mitochondria ◽  
Skeletal Muscle Mitochondria ◽  
Nadh Oxidase Activity ◽  
Phosphorylation Rate

Studies on oxidative phosphorylation revealed that, in frog skeletal muscle mitochondria (SKMM) from the thigh, the adenosine diphosphate/oxygen ratio (ADP/O) was 2.8 +/- 0.1 SE, and the respiratory control ratio was 9.5 +/- 0.9, with pyruvate/malate as the substrate. Oxygen uptake rate (Qo2) was 225 mumol O2 per minute per gram mitochondrial protein +/- 13; phosphorylation rate (ADP/O X Qo2 X 2) was 1,230 mumol ADP phosphorylation per minute per gram mitochondrial protein +/- 77; and the phosphorylation capacity (phosphorylation rate times tissue mitochondrial protein content) was 3.6 mumol ADP phosphorylated per gram wet weight of muscle +/- 0.2. Tissue mitochondrial protein content was determined by the measurement of nicotinamide adenine dinucleotide (NADH) oxidase activity. Electron microscopy (EM) revealed intact, isolated, energized twisted mitochondria of a condensed form. Frog sartorius muscle mitochondria gave similar oxidative phosphorylation parameters when investigated independently of the rest of the thigh. These values of SKMM respiration from the frog are similar to those values obtained from pigeon and rabbit heart and rat skeletal muscles. However, because of the low NADH-oxidase activity indicating reduced mitochondrial content (this was verified in low-magnification EM pictures), phosphorylation capacity was significantly reduced in frog skeletal muscle mitochondria.

scholarly journals Differential effects of endurance training and creatine depletion on regional mitochondrial adaptations in rat skeletal muscle

2000 ◽  
Vol 350 (2) ◽  
pp. 547-553 ◽  
Author(s):  
Damien ROUSSEL ◽  
Florence LHENRY ◽  
Laurent ECOCHARD ◽  
Brigitte SEMPORE ◽  
Jean-Louis ROUANET ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Oxidative Phosphorylation ◽  
Endurance Training ◽  
Oxidative Capacity ◽  
Mitochondrial Oxidative Phosphorylation ◽  
Sedentary Control ◽  
Muscle Mitochondria ◽  
F1 Atpase ◽  
Skeletal Muscle Mitochondria ◽  
Subsarcolemmal Mitochondria

To examine the combined effects of 2-week endurance training and 3-week feeding with β-guanidinopropionic acid (GPA) on regional adaptability of skeletal muscle mitochondria, intermyofibrillar mitochondria (IFM) and subsarcolemmal mitochondria (SSM) were isolated from quadriceps muscles of sedentary control, trained control, sedentary GPA-fed and trained GPA-fed rats. Mitochondrial oxidative phosphorylation was assessed polarographically by using pyruvate plus malate, succinate (plus rotenone), and ascorbate plus N,N,N´,N´-tetramethyl-p-phenylenediamine (TMPD) (plus antimycin) as respiratory substrates. Assays of cytochrome c oxidase and F1-ATPase activities were also performed. In sedentary control rats, IFM exhibited a higher oxidative capacity than SSM, whereas F1-ATPase activities were similar. Training increased the oxidative phosphorylation capacity of mitochondria with both pyruvate plus malate and ascorbate plus TMPD as substrates, with no differences between IFM and SSM. In contrast, the GPA diet mainly improved the overall SSM oxidative phosphorylation capacity, irrespective of the substrate used. Finally, the superimposition of training to feeding with GPA strongly increased both oxidase and enzymic activities in SSM, whereas no cumulative effects were found in IFM mitochondria. It therefore seems that endurance training and feeding with GPA, which are both known to alter the energetic status of the muscle cell, might mediate distinct biochemical adaptations in regional skeletal muscle mitochondria.

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