Calcium dependent ATP losses in intact red blood cells without cellular accumulations of calcium

1977 ◽  
Vol 35 (1) ◽  
pp. 309-318 ◽  
Author(s):  
Gordon A. Plishker ◽  
Hillel J. Gitelman
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Calcium Dependent

Oxidation-induced calcium-dependent dehydration of normal human red blood cells

2007 ◽  
Vol 41 (5) ◽  
pp. 536-545 ◽  
Author(s):  
Irina M. Shcherbachenko ◽  
Irina L. Lisovskaya ◽  
Vladimir P. Tikhonov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Calcium Dependent ◽  
Human Red Blood Cells ◽  
Normal Human

scholarly journals Role of calcium in volume regulation by dog red blood cells.

1975 ◽  
Vol 65 (1) ◽  
pp. 84-96 ◽  
Author(s):  
J C Parker ◽  
H J Gitelman ◽  
P S Glosson ◽  
D L Leonard
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Sodium Concentration ◽  
Calcium Flux ◽  
Osmotic Swelling ◽  
Calcium Dependent ◽  
Sodium Extrusion ◽  
Calcium Permeability ◽  
External Calcium

Dog red blood cells (RBC) are shown to regulate their volume in anisosmotic media. Extrusion of water from osmotically swollen cells requires external calcium and is associated with net outward sodium movement. Accumulation of water by osmotically shrunken cells is not calcium dependent and is associated with net sodium uptake. Net movements of calcium are influenced by several variables including cell volume, pH, medium sodium concentration, and cellular sodium concentration. Osmotic swelling of cells increases calcium permeability, and this effect is diminished at acid pH. Net calcium flux in either direction between cells and medium is facilitated when the sodium concentrations is low in the compartment from which calcium moves and/or high in the compartment to which calcium moves. The hypothesis is advanced that energy for active sodium extrusion in dog RBC comes from passive, inward flow of calcium through a countertransport mechanism.

Dehydration of mature and immature sickle red blood cells during fast oxygenation/deoxygenation cycles: role of KCl cotransport and extracellular calcium

Blood ◽  
2000 ◽  
Vol 95 (6) ◽  
pp. 2164-2168 ◽  
Author(s):  
Anthony J. McGoron ◽  
Clinton H. Joiner ◽  
Mary B. Palascak ◽  
William J. Claussen ◽  
Robert S. Franco
Keyword(s):  
Red Blood Cells ◽  
Transferrin Receptor ◽  
Blood Cells ◽  
Density Increase ◽  
Calcium Dependent ◽  
Kcl Cotransport ◽  
Ambient Oxygen ◽  
Important Pathway ◽  
Sickle Red Blood Cells

Abstract Sickle red blood cells (RBC) become dehydrated as a consequence of potassium loss. This process depends at least partly on deoxygenation and may be influenced by the presence of oxygenation/deoxygenation cycles and the frequency of cycling. In this study, sickle RBC were subjected to approximately 180 oxygenation/deoxygenation cycles during 4 hours to evaluate RBC dehydration with cycle periods more similar to in vivo cycles than those in previous studies. A continuous-flow, steady-state apparatus circulated a dilute RBC suspension through gas-permeable silicone tubing with segments that were exposed to either nitrogen or ambient oxygen. The percentage of sickling and partial pressure of oxygen were measured by means of sampling ports in the deoxygenation and oxygenation regions. The density increase (dehydration) of young (transferrin receptor-positive) and mature (transferrin receptor-negative) RBC and the requirements for calcium and chloride were evaluated. Density increase correlated with the percentage of sickled cells at the deoxygenation sampling port and was observed only in the presence of calcium, thereby implicating the calcium-dependent potassium channel (Gardos pathway). Density increase was not dependent on the presence of chloride, making it unlikely that KCl cotransport was an important pathway under these conditions.

Iodoacetic acid inhibition of calcium-dependent potassium efflux in red blood cells

1985 ◽  
Vol 248 (5) ◽  
pp. C419-C424 ◽  
Author(s):  
G. A. Plishker
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Protein Band ◽  
Iodoacetic Acid ◽  
Specific Protein ◽  
Metabolic Inhibitor ◽  
Potassium Efflux ◽  
Calcium Dependent ◽  
Irreversible Inhibition ◽  
Cellular Ca

The metabolic inhibitor, iodoacetic acid (IAA), has commonly been used to increase Ca-dependent K efflux in red blood cells. It is thought that this effect of IAA involves the irreversible inhibition of glyceraldehyde-phosphate dehydrogenase (EC 1.2.1.12), resulting in the energy depletion of the cell. Without energy, active transport stops, and the K loss is enhanced both by increasing cellular Ca and by preventing K reuptake. The present study shows that in addition to this metabolic effect, which increases Ca-dependent K efflux, IAA also inhibits this efflux. This inhibition is irreversible and is not related to the ATP or Ca concentrations of the cells. The carboxymethylation of a specific protein band correlates with IAA inhibition of K efflux.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

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