Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Electron microscopic study on the phagocytic lining cells in the cavernous body of the lamprey gill

1978 ◽  
Vol 36 (2) ◽  
pp. 448-449
Author(s):  
Kazuhito Yamaguchi ◽  
Kazuhiko Awaya
Keyword(s):  
Electron Microscope ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Embedded Blocks ◽  
Ultrathin Sections ◽  
Branchial Artery ◽  
Scanning Electron ◽  
Cavernous Body

The lining cells in the cavernous body, which is a branch of the afferent branchial artery, of lamprey gills were studied with transmission (TEM) and scanning electron microscope (SEM).Adult and larval lampreys, Lampertra planeri, were used in this study. Lamprey gills removed after perfusion were fixed in 2 % glutaraldehyde/formaldehyde (pH 7. 4) for 1 hour and postfixed in 1 % osmic acid. For TEM, the gills were dehydrated in ethanol and embedded in epon 812. Ultrathin sections were doubly stained with uranyl acetate and lead citrate. For SEM, most of the gills were dehydrated in ethanol and isoamylacetate and cracked in liquid nitrogen. Some of the gills dehydrated were embedded in stylene monomer. Stylene embedded blocks were cracked with a hummer and dissolved in propylene oxide. All the specimens were dried in a critical point dryer and coated 100 Å thickness with gold-paladium.The cavernous body is composed of trabeculae, between which are blood spaces, the lacunae.

Freeze-etch studies on the gametocytes of Plasmodium falciparum

1983 ◽  
Vol 41 ◽  
pp. 566-567
Author(s):  
Charles A.M. Meszoely ◽  
Eric F. Erbe ◽  
Russell L. Steere ◽  
Timothy Palmer ◽  
Richard L. Beaudoin
Keyword(s):  
Plasmodium Falciparum ◽  
Life Cycle ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Freeze Fracture ◽  
Malarial Parasite ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Food Vacuoles ◽  
Ultrathin Sections

The Erythrocytic stages of the Honduras strain of the malarial parasite Plasmodium falciparum were maintained in tissue culture medium RPMI 1640. Red blood cells containing gametocytes and other erythrocytic stages were fixed in 2% glutaraldehyde, and cryoprotected in 30% glycerol in H2O. This preparation was freeze-etched for one minute at -98°C in a modified Denton DFE-2 freeze-etch module. Stereo pairs with specimen tilt of 10 between electron micrographs were obtained with a JEM-100B transmission electron microscope equipped with a 60° top entry goniometer stage.The fine structure of the gametocytes of malarial parasites has been studied by conventional electron microscopic methods [stained ultrathin sections (2 and 3)], but this stage of the life cycle has not been demonstated previously with freeze-fracture studies.Since the red blood cells in our preparation also contained other stages of the life cycle, the following combination of characteristics were used to differentiate the gametocytes: presence of microtubules; food vacuoles; and large size.Microtubules are not found in trophozoites, and merozoites are relatively small and do not contain food vacuoles.

Ultrastructural characteristics on capillary of giant panda

1990 ◽  
Vol 48 (3) ◽  
pp. 491-491
Author(s):  
Zeng Xiang-Yuan ◽  
Chong Hang ◽  
Lian Wei ◽  
Zhang Ke-juing
Keyword(s):  
Microscopic Observation ◽  
Giant Panda ◽  
Electron Microscopic Observation ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Lead Citrate ◽  
Capillary Diameter ◽  
Electron Microscopic ◽  
Ultrathin Sections ◽  
Ultrastructural Characteristics

Electron microscopic observation on ultrastructural characteristics of the capillary ofgiant panda was presented. The samples were taken from mesentery and another samples were taken from mucosa of intestine. They were fixed in 2.5% phosphatebuffered glutaraldehyde and 1% osmic acid, and dehydrated by graded series of acetone. Then they were embedded with Epon 812. For orientation under phasecontrast microscope, 0.5--1.0 u thick sections were prepared from the epoxyembedded material. After that, ultrathin sections (30-50) were taken with ultramicrotome LKB-V type and stained by uranyl acetate and lead citrate and examined under DXB2-12 Electron Microscope.Electron microscopic observation showed the capillary diameter in the mesentery microcirculation is larger and capillary wall is thin. The nucleus in endothelium was large and wasn't regular in the shape. Chromatin was loose in the nucleus. There was little plasma inthe endothelium and it looked loose. Organelles in the cytoplasm were little and vacuoles were more in endothelium. The distribution of plasma in the endothelium was different with endothelium of human body.

Fine structural morphology of immunocytes of some molluscs and insects

1990 ◽  
Vol 48 (3) ◽  
pp. 386-387
Author(s):  
S.G. Pal ◽  
G. Baur ◽  
B. Ghosh ◽  
S. Palit ◽  
S. Modak ◽  
...  
Keyword(s):  
Blood Cells ◽  
Phosphate Buffer ◽  
Room Temperature ◽  
Structural Information ◽  
Uranyl Acetate ◽  
Meretrix Meretrix ◽  
Lead Citrate ◽  
Bivalve Molluscs ◽  
Structural Morphology ◽  
Ultrathin Sections

In recent years some of the blood cells of several molluscs and insects are characterised as immunocytes. Similar cells from a few invertebrates from India have been looked into under conventional TEM to register the ultrastructural features. This type of study is first of its kind in the subcontinent. Immunocytes from bivalve molluscs Meretrix meretrix, Laroellidens marqinalis and two insect species, apterygote Ctenolepism a longicaudata and pterygote Gesonula punctifrons provide a new set of fine structural information which forms a basis of comparison with those studied earlier.Immunocytes have been collected from the fresh live species of bivalve molluscs and insects obtained locally at Calcutta. These were fixed in icecold 2% glutaraldehyde in 0.1M phosphate buffer (pH 7.2-7.4) for 1-2 hours at 4-5°C. Subseguently pellets were post-osmicated in 1% OsO4 at room temperature for 1-2 hours. Following dehydration these were embedded in Araldite mixture in plastic capsules and polymerization was effected for 2 days at 60°C. Ultrathin sections were cut in a ultrotome and sections were double stained with Uranyl acetate and lead citrate. These were viewed in a TEM.

Electron Microscopic Quantitation of Hemoglobin S Polymer in SS Red Blood Cells and Rheological Correlation

1989 ◽  
Vol 565 (1 Sickle Cell D) ◽  
pp. 409-412
Author(s):  
A. ANNE KAPERONIS ◽  
ROBERT G. KING ◽  
JEANNE A. SMITH ◽  
SHU CHIEN
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Electron Microscopic ◽  
Hemoglobin S

scholarly journals Acquired hemoglobin C secondary to transfusion with antigen-matched red blood cells

2013 ◽  
Vol 29 (3) ◽  
pp. 187-188 ◽  
Author(s):  
David P. Arps ◽  
Donald A. Giacherio ◽  
Laura L. Cooling
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hemoglobin C

Immunohistochemical Identification and Electron Microscopic Study on Early Migrating Neural Crest Cells in the Chick Embryo

1997 ◽  
Vol 3 (S2) ◽  
pp. 177-178
Author(s):  
M. Monteagudo de la Rosa ◽  
M. González-Santander Martínez ◽  
G. Martinez Cuadrado ◽  
R. González Santander
Keyword(s):  
Neural Crest ◽  
Neural Tube ◽  
Early Stage ◽  
Neural Crest Cells ◽  
Uranyl Acetate ◽  
Electron Microscopic ◽  
Approaches To Study ◽  
Ultrathin Sections ◽  
Transient Structure ◽  
Immunohistochemical Identification

Just after neural fold fusion to form the neural tube, neural crest cells detach from the neural crest, a transient structure located in the dorsal region of the neural tube. Neural crest cells migrate and differentiate into many structures and cells. But the underlying controls of this detachment and initiation of emigration are unknown. Neural crest cells are usually not morphologically distinct from the adjacent neural epithelium (neural tube) and epidermal ectoderm (epiblast) flanking them. We are combining morphological and immunohistochemical approaches to study neural crest cells in their early stage of detachment from the neural crest.Hamburger and Hamilton (1951) stages 9 to 12 White Leghorn chick embryos. Fixation in 2.5% glutaraldehyde - 0.5% tanic acid and postfixation in 1% osmium tetroxide. Embryos contrasted in bloc using uranyl acetate and embedded in araldite. Semithin transversal sections stained with toluidine blue for light microscopy. Ultrathin sections contrasted with lead citrate.

The fine structure of ascus development in Lasiobolus monascus (Pezizales)

1978 ◽  
Vol 56 (7) ◽  
pp. 862-872 ◽  
Author(s):  
James W. Kimbrough ◽  
Gerald L. Benny
Keyword(s):  
Fine Structure ◽  
Uranyl Acetate ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Stalk Cell ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Ultrathin Sections ◽  
Microscopic Inspection ◽  
Physical And Chemical

Ultrastructural and cytochemical studies on the ascus of Lasiobolus monascus are presented. Apothecia in various stages of development were obtained in culture and prepared for both light and electron microscopic observations. Ultrathin sections for electron microscopic inspection were often treated with silver methenamine to enhance wall characteristics. Ascus development was followed from fertilization to maturity.In this species, the ascogonium enlarges after fertilization to become the ascus mother cell. Two pores are present in the young ascus, one connecting it to the antheridium and another between the ascus and stalk cell. The ultrastructural features of these pores in the young and maturing ascus are described. During ascus enlargement, as many as four wall layers are found when poststained with silver methenamine. Only two layers are clearly distinguishable when poststained with uranyl acetate and lead citrate. The apical zone of dehiscence is characterized by a distinct annular swelling which appears during early ascosporogenesis. By spore maturation, this swelling is not evident either at the light or electron microscopic level. Instead, there appear to be both physical and chemical changes in the area of dehiscence. The wall is distinctly thinner and much more electron transparent in the area of dehiscence when treated with silver methanamine.

scholarly journals Electron Microscopic Observation of Red Blood Cells in Paroxysmal Nocturnal Hemoglobinuria

1967 ◽  
Vol 93 (2) ◽  
pp. 131-137
Author(s):  
Akira B. Miura ◽  
Akira Shibata ◽  
Sadao Takase ◽  
Seiju Onodera ◽  
Atsuo Suzuki ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Microscopic Observation ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Electron Microscopic Observation ◽  
Electron Microscopic

scholarly journals Intraerythrocytic Hemoglobin Crystals in Sickle Cell-Hemoglobin C Disease

Blood ◽  
1965 ◽  
Vol 25 (2) ◽  
pp. 218-223 ◽  
Author(s):  
L. W. DIGGS ◽  
ANN BELL
Keyword(s):  
Red Blood Cells ◽  
Sickle Cell ◽  
Blood Cells ◽  
Blood Corpuscle ◽  
Hemoglobin C ◽  
Blood Smears

Abstract On 70 per cent of the blood smears from 60 cases of electrophoretically proven sickle cell-hemoglobin C disease, there is observed a misshapen erythrocyte that contains condensed hemoglobin crystals which are dark-hued, homogeneous and elongated and which have parallel sides with one end terminating in a pyramid or rounded shape. A red blood corpuscle may have multiple protuberances at varying angles to each other. The incidence of intracellular hemoglobin crystals was found to be 0-24 per 1000 red blood cells with an average of 3.2/1000. Recognition of this unusual morphology is presumptive evidence of sickle cell-hemoglobin C and warrants examination by electrophoretic procedures.

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