The effect of cigarette-smoke condensate on the in vitro fixation of benzo(a)pyrene on DNA

1972 ◽  
Vol 28 (8) ◽  
pp. 932-933 ◽  
Author(s):  
K. Alexandrov ◽  
C. Frayssinet
Keyword(s):  
Cigarette Smoke ◽  
Cigarette Smoke Condensate

scholarly journals Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium

2015 ◽  
Vol 87 (2) ◽  
pp. 997-1005 ◽  
Author(s):  
Jinqiang Hu ◽  
Tao Wei ◽  
Siwen Sun ◽  
Aijing Zhao ◽  
Chunping Xu
Keyword(s):  
Cigarette Smoke ◽  
Carbohydrate Composition ◽  
Cigarette Smoke Condensate ◽  
Essential Factor ◽  
Standard Strain ◽  
Acetic Acid Production ◽  
Bifidobacterium Animalis ◽  
Molecular Determination

The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium. Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC). The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC. The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production. Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination. By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition. The molecular weight mass of Fr-I changed from 3.33×105 g/mol (without CSC) to 2.99×105 (with CSC). In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.

Humic-like substances in cigarette smoke condensate and lung tissue of smokers

1994 ◽  
Vol 266 (4) ◽  
pp. L382-L388 ◽  
Author(s):  
A. J. Ghio ◽  
J. Stonehuerner ◽  
D. R. Quigley
Keyword(s):  
Free Radical ◽  
Cigarette Smoke ◽  
Lung Tissue ◽  
Cigarette Smoke Exposure ◽  
Cigarette Smoke Condensate ◽  
Free Radical Generation ◽  
Carboxylate Content ◽  
Radical Generation

Deposition of pigmented matter in the lower respiratory tract correlates with the extent of emphysema in smokers as well as with free radical generation and iron accumulation. Pulmonary emphysema is postulated to be mediated by free radical generation which is either directly or indirectly associated with cigarette smoke exposure. The hypothesis was tested that 1) incomplete combustion of tobacco yields humic-like substances (HLS) which 2) deposit in the lung as pigmented particulates, 3) complex iron cations in vitro and in vivo, and 4) have a capacity to catalyze oxidant formation. HLS, isolated by alkali extraction of cigarette smoke condensate (CSC) (Tobacco Health Research Institute, University of Kentucky), demonstrated a high carbon and low carboxylate content on elemental and functional group analyses, respectively, compared with values for HLS sequestered from soils. The HLS isolated from CSC had a capacity to complex iron in vitro and accumulated the metal in vivo after intratracheal instillation in an animal model. Both HLS and its iron complex generated free radicals, and some portion of this oxidant generation was metal dependent. Lung tissue collected at autopsy from smokers contained HLS with an infrared spectrum almost identical to that of the material isolated from CSC. Associations between particulate deposition, metal accumulation, and free radical generation suggest a possible role of HLS in the induction of lung disease following cigarette exposure.

scholarly journals Cigarette smoke condensate-induced transformation of normal human breast epithelial cells in vitro

Oncogene ◽  
2004 ◽  
Vol 23 (35) ◽  
pp. 5880-5889 ◽  
Author(s):  
Satya Narayan ◽  
Aruna S Jaiswal ◽  
Diana Kang ◽  
Pratima Srivastava ◽  
Gokul M Das ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Cigarette Smoke Condensate ◽  
Human Breast ◽  
Breast Epithelial Cells ◽  
Normal Human Breast ◽  
Normal Human ◽  
Breast Epithelial ◽  
Human Breast Epithelial Cells

In vitro studies of biological effects of cigarette smoke condensate

1985 ◽  
Vol 157 (2-3) ◽  
pp. 169-180 ◽  
Author(s):  
Margareta Curvall ◽  
Tommy Jansson ◽  
Bertil Pettersson ◽  
Annica Hedin ◽  
Curt R. Enzell
Keyword(s):  
Cigarette Smoke ◽  
Biological Effects ◽  
In Vitro Studies ◽  
Cigarette Smoke Condensate

In vitro and in vivo immunotoxic and immunomodulatory effects of nonsupplemented and selenium-supplemented cigarette smoke condensate

2004 ◽  
Vol 58 (2) ◽  
pp. 90-94 ◽  
Author(s):  
Pascale Nguyen Van Binh ◽  
Dong Zhou ◽  
Françoise Baudouin ◽  
Chantal Martin ◽  
Martine Radionoff ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Cigarette Smoke Condensate ◽  
Immunomodulatory Effects

In vitro studies of biological effects of cigarette smoke condensate

1986 ◽  
Vol 169 (3) ◽  
pp. 129-139 ◽  
Author(s):  
Tommy Jansson ◽  
Margareta Curvall ◽  
Annica Hedin ◽  
Curt R. Enzell
Keyword(s):  
Cigarette Smoke ◽  
Biological Effects ◽  
In Vitro Studies ◽  
Cigarette Smoke Condensate

Modulation of the mutagenic activity of cigarette smoke, cigarette smoke condensate and benzo[a]pyrene in vitro and in vivo

Mutagenesis ◽  
1994 ◽  
Vol 9 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Roumen Balansky ◽  
Zvetanka Mircheva ◽  
Penka Blagoeva
Keyword(s):  
Cigarette Smoke ◽  
Mutagenic Activity ◽  
Cigarette Smoke Condensate
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