Ultrastructure of meiosis in the hollyhock rust fungus,Puccinia malvacearum I. Prophase I — Prometaphase I

PROTOPLASMA ◽  
1981 ◽  
Vol 108 (3-4) ◽  
pp. 225-244 ◽  
Author(s):  
K. L. O'Donnell ◽  
D. J. McLaughlin
Keyword(s):  
Rust Fungus ◽  
Prophase I ◽  
Puccinia Malvacearum

Ultrastructure of meiosis in the hollyhock rust fungus,Puccinia malvacearum II. Metaphase I—Telophase I

PROTOPLASMA ◽  
1981 ◽  
Vol 108 (3-4) ◽  
pp. 245-263 ◽  
Author(s):  
K. L. O'Donnell ◽  
D. J. McLaughlin
Keyword(s):  
Rust Fungus ◽  
Puccinia Malvacearum ◽  
Metaphase I

Ultrastructural Observations on the Rust Fungus Puccinia malvacearum in Malva sylvestris ssp. mauritiana

Plant Biology ◽  
2001 ◽  
Vol 3 (4) ◽  
pp. 437-442 ◽  
Author(s):  
B. Classen ◽  
F. Amelunxen ◽  
W. Blaschek
Keyword(s):  
Rust Fungus ◽  
Malva Sylvestris ◽  
Puccinia Malvacearum

Ultrastructure of meiosis in the hollyhock rust fungus,Puccinia malvacearum

PROTOPLASMA ◽  
1981 ◽  
Vol 108 (3-4) ◽  
pp. 265-288 ◽  
Author(s):  
K. L. O'Donnell ◽  
D. J. McLaughlin
Keyword(s):  
Rust Fungus ◽  
Puccinia Malvacearum

High-pressure freezing and freeze substitution of teliospores of the rust fungus Gymnosporangium clavipes

1990 ◽  
Vol 48 (3) ◽  
pp. 692-693
Author(s):  
Robert W. Roberson
Keyword(s):  
High Pressure ◽  
Room Temperature ◽  
Pathogenic Fungus ◽  
Rust Fungus ◽  
Freeze Substitution ◽  
Ice Crystals ◽  
High Pressure Freezing ◽  
Thin Walled Structures ◽  
Cytological Changes ◽  
Dextran Solution

The use of cryo-techniques for the preparation of biological specimens in electron microscopy has led to superior preservation of ultrastructural detail. Although these techniques have obvious advantages, a critical limitation is that only 10-40 μm thick cells and tissue layers can be frozen without the formation of distorting ice crystals. However, thicker samples (600 μm) may be frozen well by rapid freezing under high-pressure (2,100 bar). To date, most work using cryo-techniques on fungi have been confined to examining small, thin-walled structures. High-pressure freezing and freeze substitution are used here to analysis pre-germination stages of specialized, sexual spores (teliospores) of the plant pathogenic fungus Gymnosporangium clavipes C & P.Dormant teliospores were incubated in drops of water at room temperature (25°C) to break dormancy and stimulate germination. Spores were collected at approximately 30 min intervals after hydration so that early cytological changes associated with spore germination could be monitored. Prior to high-pressure freezing, the samples were incubated for 5-10 min in a 20% dextran solution for added cryoprotection during freezing. Forty to 50 spores were placed in specimen cups and holders and immediately frozen at high pressure using the Balzers HPM 010 apparatus.

Etoposide results in follicle loss in the fetal mouse ovary, but does not block the ability of oocytes to progress through prophase I of meiosis

2016 ◽  
Author(s):  
Agnes Stefansdottir ◽  
Zoe Johnston ◽  
Nicola Powles-Glover ◽  
Richard Anderson ◽  
Ian Adams ◽  
...  
Keyword(s):  
Mouse Ovary ◽  
Fetal Mouse ◽  
Prophase I

The influence of Robertsonian translocations on semen parameters

2020 ◽  
pp. 87-88
Author(s):  
М.В. Андреева ◽  
М.И. Штаут ◽  
Т.М. Сорокина ◽  
Л.Ф. Курило ◽  
В.Б. Черных
Keyword(s):  
Male Infertility ◽  
Semen Parameters ◽  
Meiotic Arrest ◽  
Robertsonian Translocations ◽  
Prophase I ◽  
Infertile Men ◽  
Fertility Problems ◽  
Spermatogenesis Impairment

Обследованы 19 мужчин с нарушением фертильности, носителей транслокаций rob(13;14) и rob(13;15). Показано, что нарушение репродуктивной функции обусловлено блоком сперматогенеза в профазе I мейоза, приводящего к азооспермии или олигоастенотератозооспермии и мужскому бесплодию. We examined 19 infertile men, carriers of translocations rob (13;14) and rob (13;15). We assume that fertility problems are resulted from spermatogenesis impairment because of meiotic arrest at prophase I stages, that leads to azoospermia or oligoastenoteratozoospermia and male infertility.

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