Identification of human chromosome region 3p14.2–21.3-specific YAC clones using Alu-PCR products from a radiation hybrid

1994 ◽  
Vol 20 (2) ◽  
pp. 137-142
Author(s):  
Thomas S. Siden ◽  
Johan Kumlien ◽  
Timothy Drumheller ◽  
Scott E. Smith ◽  
Dan Röhme ◽  
...  
Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 327-339 ◽  
Author(s):  
O Riera-Lizarazu ◽  
M I Vales ◽  
E V Ananiev ◽  
H W Rines ◽  
R L Phillips

Abstract In maize (Zea mays L., 2n = 2x = 20), map-based cloning and genome organization studies are often complicated because of the complexity of the genome. Maize chromosome addition lines of hexaploid cultivated oat (Avena sativa L., 2n = 6x = 42), where maize chromosomes can be individually manipulated, represent unique materials for maize genome analysis. Maize chromosome addition lines are particularly suitable for the dissection of a single maize chromosome using radiation because cultivated oat is an allohexaploid in which multiple copies of the oat basic genome provide buffering to chromosomal aberrations and other mutations. Irradiation (gamma rays at 30, 40, and 50 krad) of a monosomic maize chromosome 9 addition line produced maize chromosome 9 radiation hybrids (M9RHs)—oat lines possessing different fragments of maize chromosome 9 including intergenomic translocations and modified maize addition chromosomes with internal and terminal deletions. M9RHs with 1 to 10 radiation-induced breaks per chromosome were identified. We estimated that a panel of 100 informative M9RHs (with an average of 3 breaks per chromosome) would allow mapping at the 0.5- to 1.0-Mb level of resolution. Because mapping with maize chromosome addition lines and radiation hybrid derivatives involves assays for the presence or absence of a given marker, monomorphic markers can be quickly and efficiently mapped to a chromosome region. Radiation hybrid derivatives also represent sources of region-specific DNA for cloning of genes or DNA markers.


Genomics ◽  
1998 ◽  
Vol 48 (1) ◽  
pp. 93-99 ◽  
Author(s):  
Ya-Ping Yang ◽  
Caird E. Rexroad ◽  
Jörg Schläpfer ◽  
James E. Womack

Gene ◽  
1993 ◽  
Vol 136 (1-2) ◽  
pp. 177-183 ◽  
Author(s):  
Donald T. Moir ◽  
Thomas E. Dorman ◽  
Xue Feiyu ◽  
Nancy Shui-Fong Ma ◽  
Vincent P. Stanton ◽  
...  

1999 ◽  
Vol 84 (3-4) ◽  
pp. 188-189 ◽  
Author(s):  
L. Bartoloni ◽  
J.-L.C. Blouin ◽  
A.J. Sainsbury ◽  
A. Gos ◽  
M.A. Morris ◽  
...  

1988 ◽  
Vol 48 (1) ◽  
pp. 63-64 ◽  
Author(s):  
T. Tokino ◽  
H. Satoh ◽  
M.C. Yoshida ◽  
T. Ochiya ◽  
K. Matsubara

1988 ◽  
Vol 47 (1-2) ◽  
pp. 37-41 ◽  
Author(s):  
L. Pajunen ◽  
R. Myllylä ◽  
T. Helaakoski ◽  
T. Pihlajaniemi ◽  
K. Tasanen ◽  
...  

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