A new method for the determination of mobile phase volume in normal and reversed-phase liquid chromatography

1988 ◽  
Vol 25 (4) ◽  
pp. 288-294 ◽  
Author(s):  
M. Shibukawa ◽  
N. Ohta
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
Reversed Phase ◽  
New Method ◽  
Phase Volume ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Mobile Phase Volume

scholarly journals Determination of Methomyl in Insecticidal Formulations by Reversed-Phase Liquid Chromatography: Collaborative Study

1996 ◽  
Vol 79 (4) ◽  
pp. 941-948
Author(s):  
James E Conaway ◽  
J B Audino ◽  
E Bane ◽  
S K Carrigan ◽  
R Glinsky ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Coefficient Of Variation ◽  
Mobile Phase ◽  
Collaborative Study ◽  
Internal Standard ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reversed-phase liquid chromatographic (LC) method for methomyl was studied. Twelve collaborators analyzed 3 solid and 4 liquid formulations on both a Zorbax octadecylsilane (ODS) column and a similar column of their choice. Methomyl and the internal standard were separated by using a mobile phase consisting of approximately 8% acetonitrile in water, which was monitored at 254 nm. The coefficient of variation on the Zorbax column ranged from 0.70 to 5.23%, while the range on the collaborators' house columns was 1.08 to 6.01%. Results with the Zorbax ODS column fell within the 5% 2-tail limits, and 10 of 11 collaborators' results fell within these limits on house columns. The LC method for determination of methomyl in insecticidal formulations has been adopted first action by AOAC INTERNATIONAL.

Determination of erythrocytic polyamines by reversed-phase liquid chromatography

1991 ◽  
Vol 37 (12) ◽  
pp. 2117-2120 ◽  
Author(s):  
Lucile Gerbaut
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Water Gradient ◽  
Phase Liquid ◽  
Dansyl Derivatives

Abstract A simple and rapid semiautomated procedure for determining polyamines in erythrocytes by high-performance liquid chromatography is described. Putrescine, spermidine, and spermine are converted to fluorescent dansyl derivatives, extracted with cyclohexane, and separated in <10 min on a reversed-phase C18 ODS column, with an acetonitrile-water gradient as the mobile phase. The method showed a coefficient of variation of 2.73% for spermidine and 3.27% for spermine. The respective reference values, evaluated in 10 healthy patients, were 7.88 (SD 2.09) and 5.42 (SD 1.55) μmol/L of packed erythrocytes. Only negligible amounts of putrescine were found.

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