Articular cartilage destruction in experimental inflammatory arthritis: insulin-like growth factor-1 regulation of proteoglycan metabolism in chrondrocytes

1996 ◽  
Vol 28 (12) ◽  
pp. 835-857 ◽  
Author(s):  
Pernette J. Verschure ◽  
Cornelis J. F. Van Noorden ◽  
Jan Van Marle ◽  
Wim B. Van Den Berg
Keyword(s):  
Articular Cartilage ◽  
Growth Factor ◽  
Inflammatory Arthritis ◽  
Cartilage Destruction ◽  
Insulin Like Growth Factor

scholarly journals Insulin-like growth factor-I enhances cell-based repair of articular cartilage

2002 ◽  
Vol 84-B (2) ◽  
pp. 276-288 ◽  
Author(s):  
L. A. Fortier ◽  
H. O. Mohammed ◽  
G. Lust ◽  
A. J. Nixon
Keyword(s):  
Articular Cartilage ◽  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Growth Factor I

Mechanical and Physicochemical Regulation of the Action of Insulin-Like Growth Factor-I on Articular Cartilage

2000 ◽  
Vol 379 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Lawrence J. Bonassar ◽  
Alan J. Grodzinsky ◽  
Aneetha Srinivasan ◽  
Salomon G. Davila ◽  
Stephen B. Trippel
Keyword(s):  
Articular Cartilage ◽  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Growth Factor I

scholarly journals Localization and quantification of the insulin-like growth factor-1 receptor in mouse articular cartilage by confocal laser scanning microscopy.

1994 ◽  
Vol 42 (6) ◽  
pp. 765-773 ◽  
Author(s):  
P J Verschure ◽  
J van Marle ◽  
L A Joosten ◽  
W B Van den Berg
Keyword(s):  
Articular Cartilage ◽  
Growth Factor ◽  
Confocal Laser Scanning Microscopy ◽  
Fluorescence Intensity ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Insulin Like Growth Factor

Insulin-like growth factor-1 (IGF-1) is an essential anabolic growth factor in the regulation of cartilage metabolism and exerts its effects by binding to the IGF-1 Type 1 receptor on the chondrocyte membrane. We have localized and quantified in situ IGF-1 receptor expression in intact articular cartilage of normal mice. The IGF-1 receptor was detected immunohistochemically with antibodies to the IGF-1 receptor and visualized with conventional light microscopy and confocal laser scanning microscopy (CLSM). CLSM analysis enabled us to distinguish IGF-1 receptor immunoreactivity on the chondrocyte cell membrane from intracellular staining. We have established two approaches to quantify in confocal images low levels of fluorescence intensity of the immunolocalized IGF-1 receptor at the chondrocyte membrane, i.e., mean pixel measurement and area measurement. The majority of IGF-1 receptor fluorescence intensity was localized on chondrocytes in the middle and deeper zones of cartilage, whereas chondrocytes in the surface zone exhibited negligible fluorescence. The variable distribution of IGF-1 receptor in chondrocytes of articular cartilage suggests that effects of IGF-1 on chondrocytes may be distinctly heterogeneous in the different mouse articular cartilage zones.

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