Electron microscopic autoradiographic study on immune complex formation in rat glomerulus with anti-heparan sulfate proteoglycan antibody

1994 ◽  
Vol 27 (3-4) ◽  
pp. 358-359
Author(s):  
Hirofumi Makino ◽  
Naoki Kashihara ◽  
Zensuke Ota ◽  
Yashpal S. Kanwar
Keyword(s):  
Complex Formation ◽  
Heparan Sulfate ◽  
Immune Complex ◽  
Autoradiographic Study ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Electron Microscopic ◽  
Immune Complex Formation

scholarly journals Fibroblasts that proliferate near denervated synaptic sites in skeletal muscle synthesize the adhesive molecules tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan.

1989 ◽  
Vol 108 (5) ◽  
pp. 1873-1890 ◽  
Author(s):  
C L Gatchalian ◽  
M Schachner ◽  
J R Sanes
Keyword(s):  
Skeletal Muscle ◽  
Heparan Sulfate ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
3T3 Cells ◽  
Denervated Muscle ◽  
Electron Microscopic ◽  
Cultured Fibroblasts ◽  
Adhesive Molecules

Four adhesive molecules, tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan, accumulate in interstitial spaces near synaptic sites after denervation of rat skeletal muscle (Sanes, J. R., M. Schachner, and J. Covault. 1986. J. Cell Biol. 102:420-431). We have now asked which cells synthesize these molecules, and how this synthesis is regulated. Electron microscopy revealed that mononucleated cells selectively accumulate in perisynaptic interstitial spaces beginning 2 d after denervation. These cells were identified as fibroblasts by ultrastructural and immunohistochemical criteria; [3H]thymidine autoradiography revealed that their accumulation results from local proliferation. Electron microscopic immunohistochemistry demonstrated that N-CAM is associated with the surface of the fibroblasts, while tenascin(J1) is associated with collagen fibers that abut fibroblasts. Using immunofluorescence and immunoprecipitation methods, we found that fibroblasts isolated from perisynaptic regions of denervated muscle synthesize N-CAM, tenascin(J1), fibronectin, and a heparan sulfate proteoglycan in vitro. Thus, fibroblasts that selectively proliferate in interstitial spaces near synaptic sites are likely to be the cellular source of the interstitial deposits of adhesive molecules in denervated muscle. To elucidate factors that might regulate the accumulation of these molecules in vivo, we analyzed the expression of tenascin(J1) and fibronectin by cultured fibroblasts. Fibroblasts from synapse-free regions of denervated muscle, as well as skin, lung, and 3T3 fibroblasts accumulate high levels of tenascin(J1) and fibronectin in culture, showing that perisynaptic fibroblasts are not unique in this regard. However, when they are first placed in culture, fibroblasts from denervated muscle bear more tenascin(J1) than fibroblasts from innervated muscle, indicating that expression of this molecule by fibroblasts is regulated by the muscle's state of innervation; this difference is no longer apparent after a few days in culture. In 3T3 cells, accumulation of tenascin(J1) is high in proliferating cultures, depressed in confluent cultures, and reactivated in cells stimulated to proliferate by replating at low density or by wounding a confluent monolayer. Thus, synthesis of tenascin(J1) is regulated in parallel with mitotic activity. In contrast, levels of fibronectin, which increase less dramatically after denervation in vivo, are similar in fibroblasts from innervated and denervated muscle and in proliferating and quiescent 3T3 cells.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Heparan sulfate proteoglycan is present in basement membrane as a double-tracked structure.

1989 ◽  
Vol 37 (5) ◽  
pp. 597-602 ◽  
Author(s):  
S Inoue ◽  
D Grant ◽  
C P Leblond
Keyword(s):  
Basement Membrane ◽  
Heparan Sulfate ◽  
Core Protein ◽  
Heparan Sulfate Proteoglycan ◽  
Basement Membranes ◽  
Sulfate Proteoglycan ◽  
Electron Microscopic ◽  
Gold Particles ◽  
Parallel Lines ◽  
Reichert's Membrane

Basement membranes contain 4.5-nm wide sets of two parallel lines, along which short prongs called "spikes" occur at regular intervals. The nature of this structure, referred to as "double tracks," was investigated in Lowicryl sections of mouse kidney and rat Reichert's membrane immunolabeled for basement membrane components using secondary antibodies conjugated to 5-nm gold particles. When the mouse glomerular basement membrane and rat Reichert's membrane were exposed to antibodies directed to the core protein of heparan sulfate proteoglycan, 95% or more of the gold particles were over double tracks, whereas after exposure of Reichert's membrane to antisera against laminin, collagen IV, or entactin, labeling of the double tracks remained at the random level. When heparan sulfate proteoglycan was incubated in Tris buffer, pH 7.4, at 35 degrees C for 1 hr, a precipitate resulted which, on electron microscopic examination, was found to consist of 5- to 6-nm wide sets of two parallel lines along which densities were observed. Immunolabeling confirmed the presence of the proteoglycan's core protein in the sets. Since double tracks were closely similar to this structure and were labeled with the same antibodies, they were likely to be also composed of heparan sulfate proteoglycan.

Alterations in the basement membrane (heparan sulfate) proteoglycan in diabetic mice

Diabetes ◽  
1982 ◽  
Vol 31 (2) ◽  
pp. 185-188 ◽  
Author(s):  
D. H. Rohrbach ◽  
J. R. Hassell ◽  
H. K. Kleinman ◽  
G. R. Martin
Keyword(s):  
Basement Membrane ◽  
Heparan Sulfate ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Diabetic Mice

Targeting Mechanism of a Novel Liver-targeting Interferon IFN-CSP Involves Liver Heparan Sulfate Proteoglycan

2016 ◽  
Vol 13 (4) ◽  
pp. 528-533 ◽  
Author(s):  
Xuemei Lu ◽  
Jie Wang ◽  
Xiaobao Jin ◽  
Yanting Huang ◽  
Wenting Zeng ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Liver Targeting

Immunohistochemical Study of Heparan Sulfate Proteoglycan in Adenocarcinomas of the Pancreas

Pancreas ◽  
1994 ◽  
Vol 9 (6) ◽  
pp. 758-763 ◽  
Author(s):  
Zhao-hui Wang ◽  
Tadao Manabe ◽  
Gakuji Ohshio ◽  
Takashi Imamura ◽  
Tsunehiro Yoshimura ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Immunohistochemical Study ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan

scholarly journals Fatty acylation of heparan sulfate proteoglycan from human colon carcinoma cells.

1990 ◽  
Vol 265 (32) ◽  
pp. 19980-19989
Author(s):  
R V Iozzo ◽  
I Kovalszky ◽  
N Hacobian ◽  
P K Schick ◽  
J S Ellingson ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Heparan Sulfate Proteoglycan ◽  
Carcinoma Cells ◽  
Sulfate Proteoglycan ◽  
Fatty Acylation ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma
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