Identification of the DNAs of the ectomycorrhizal fungus Tricholoma bakamatsutake using specific oligonucleotide probes and PCR primers

Mycoscience ◽  
1996 ◽  
Vol 37 (3) ◽  
pp. 371-375 ◽  
Author(s):  
Yoshie Terashima ◽  
Takao Nakai
Keyword(s):  
Pcr Primers ◽  
Ectomycorrhizal Fungus ◽  
Oligonucleotide Probes ◽  
Tricholoma Bakamatsutake

How to design and optimize PCR primers and sequence-specific oligonucleotide probes for DNA typing

1993 ◽  
Vol 13 (12) ◽  
pp. 157-163
Author(s):  
Carolyn K. Hurley ◽  
Debra Kukuruga ◽  
Sandra Rosen Bronson
Keyword(s):  
Dna Typing ◽  
Pcr Primers ◽  
Oligonucleotide Probes ◽  
Sequence Specific Oligonucleotide Probes

Microsatellite sequences in a conifer, Pinus sylvestris

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1244-1248 ◽  
Author(s):  
Silja Kostia ◽  
Sirkka-Liisa Varvio ◽  
Pekka Vakkari ◽  
Pertti Pulkkinen
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Molecular Basis ◽  
Pcr Primers ◽  
Pollen Grain ◽  
Plant Genome ◽  
Oligonucleotide Probes ◽  
Genomic Libraries ◽  
Pcr Products

Scots pine (Pinus sylvestris) genomic libraries were constructed and screened with oligonucleotide probes (GT)10, (CT)10, and (AT)10. Eight microsatellites were identified from 6000 clones screened. The longest microsatellite stretch found, (GT)9(N)21(AT)24, was amplified from bud and single pollen grain samples. In order to clarify the complex amplification pattern revealed, two PCR products were sequenced. The size differences were caused both by varying repeat numbers of the microsatellite stretches and by differences in other parts of the amplified sequence. This kind of complex molecular basis of microsatellite amplification within a species has not been previously reported. Microsatellite sequences were used as PCR primers to detect polymorphisms and to estimate the abundance of microsatellites.Key words: microsatellites, Pinus sylvestris, plant genome, inter SSR–PCR.

Gluconic acid synthesis by the ectomycorrhizal fungus Tricholoma robustum

1992 ◽  
Vol 70 (1) ◽  
pp. 84-88 ◽  
Author(s):  
Koji Iwase
Keyword(s):  
Glucose Oxidase ◽  
Gluconic Acid ◽  
High Performance ◽  
Culture Medium ◽  
Acid Synthesis ◽  
Ectomycorrhizal Fungus ◽  
Tricholoma Matsutake ◽  
Mycelial Culture ◽  
High Productivity ◽  
Tricholoma Bakamatsutake

During mycelial culture of Tricholoma robustum, the medium gradually became acidified to approximately pH 3.9. High performance liquid chromatography showed that gluconic acid was secreted into the culture medium, and the amount of gluconic acid produced was measured by enzymatic analysis. Gluconic acid synthesis by all other related species, Tricholoma matsutake, Tricholoma caligatum, Tricholoma ponderosum, Tricholoma fulvocastaneum, and Tricholoma zelleri was poor, except for Tricholoma bakamatsutake, which showed relatively high productivity. Activity of glucose oxidase, which is responsible for gluconic acid production, was highest in T. robustum and second highest in T. bakamatsutake. The activity in these two species was much higher than those of other species. These results indicate that gluconic acid was synthesized from glucose by glucose oxidase in T. robustum as well as in T. bakamatsutake. Key words: ectomycorrhizal fungi, gluconic acid, glucose oxidase, Tricholoma robustum.

scholarly journals Development of oligonucleotide probes and PCR primers for detecting phylogenetic subgroups of sulfate-reducing bacteria

Microbiology ◽  
2000 ◽  
Vol 146 (7) ◽  
pp. 1693-1705 ◽  
Author(s):  
Kristian Daly ◽  
Richard J. Sharp ◽  
Alan J. McCarthy
Keyword(s):  
Pcr Primers ◽  
Sulfate Reducing Bacteria ◽  
Oligonucleotide Probes ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Relationship between cumulative heat units and fruit-body emergence of ectomycorrhizal fungus Tricholoma bakamatsutake in the fields

Mycoscience ◽  
1995 ◽  
Vol 36 (2) ◽  
pp. 187-191
Author(s):  
Yoshie Terashima ◽  
Azusa Fujiie ◽  
Kenzo Tomiya
Keyword(s):  
Fruit Body ◽  
Ectomycorrhizal Fungus ◽  
Heat Units ◽  
Tricholoma Bakamatsutake
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