Microsatellite sequences in a conifer, Pinus sylvestris

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1244-1248 ◽  
Author(s):  
Silja Kostia ◽  
Sirkka-Liisa Varvio ◽  
Pekka Vakkari ◽  
Pertti Pulkkinen
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Molecular Basis ◽  
Pcr Primers ◽  
Pollen Grain ◽  
Plant Genome ◽  
Oligonucleotide Probes ◽  
Genomic Libraries ◽  
Pcr Products

Scots pine (Pinus sylvestris) genomic libraries were constructed and screened with oligonucleotide probes (GT)10, (CT)10, and (AT)10. Eight microsatellites were identified from 6000 clones screened. The longest microsatellite stretch found, (GT)9(N)21(AT)24, was amplified from bud and single pollen grain samples. In order to clarify the complex amplification pattern revealed, two PCR products were sequenced. The size differences were caused both by varying repeat numbers of the microsatellite stretches and by differences in other parts of the amplified sequence. This kind of complex molecular basis of microsatellite amplification within a species has not been previously reported. Microsatellite sequences were used as PCR primers to detect polymorphisms and to estimate the abundance of microsatellites.Key words: microsatellites, Pinus sylvestris, plant genome, inter SSR–PCR.

The importance of being the first pollen in the strobili of Scots pine

2008 ◽  
Vol 38 (12) ◽  
pp. 2976-2980 ◽  
Author(s):  
Saila Varis ◽  
Arja Santanen ◽  
Anne Pakkanen ◽  
Pertti Pulkkinen
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Pollen Grains ◽  
Seed Orchard ◽  
Pollen Grain ◽  
Seed Orchards ◽  
Pollination Experiments ◽  
Seed Crop ◽  
Pollen Types ◽  
Pine Pollen

Timing of pollen arrival may affect the level of seeds fertilized by pollen from outside seed orchards, especially in seed orchards of Scots pine ( Pinus sylvestris L.) established in southern Finland with stock from northern populations. We performed sequential pollination experiments with Scots pine pollen collected from seed orchard clones originating from southern and northern Finland and recipient strobili in clones originating from southern Finland. When southern pollen was introduced first, seeds were sired equally by northern and southern genotypes. When northern pollen was introduced first, northern genotypes were found in 75% of the resulting seeds. When both pollen types were introduced at the same time, the resulting seed crop was split almost equally between northern and southern genotypes. These results do not unconditionally support the idea that the first pollen grain in the pollen chamber always fertilizes the ovum; instead, they suggest a more complex way of competition between pollen grains.

The development of microsatellite DNA markers for genetic analysis in Douglas-fir

2002 ◽  
Vol 32 (11) ◽  
pp. 1904-1915 ◽  
Author(s):  
Vindhya Amarasinghe ◽  
John E Carlson
Keyword(s):  
Pcr Primers ◽  
Douglas Fir ◽  
Base Pairs ◽  
Genomic Libraries ◽  
Polymorphic Loci ◽  
Microsatellite Dna Markers ◽  
Pcr Products ◽  
Conifer Tree ◽  
Flanking Sequences ◽  
Locus Allele

The microsatellite motifs AG, AC, and ATG were found to be the most abundant in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and several other conifer tree species among di-, tri-, and tetra-nucleotide simple sequence repeats (SSR). Colonies containing AG, AC, and ATG repeats were selected from enriched genomic libraries of Douglas-fir, and 603 were sequenced. Polymerase chain reaction (PCR) primers were designed from flanking sequences in 102 of the SSR clones, of which 50 primer pairs (for 10 AC-repeat microsatellites and 40 AG-repeat microsatellites) produced robust amplification products. Variability was confirmed with 24 unrelated Douglas-fir trees and Medelian segregation with 33-66 progeny from 3 full-sib populations. Forty-eight of the 50 loci were polymorphic, with a mean of 7.5 alleles per locus. Allele sizes ranged from 73 to 292 base pairs. Allele frequencies for the 48 polymorphic loci varied from 0.017 to 0.906 with mean allele frequency of 0.250. Expected heterozygosities among the polymorphic loci varied from 0.174 to 0.926, with a mean of 0.673. Additional, high molecular weight PCR products were amplified by some of the primer pairs, but they did not interfere with the scoring of alleles. Most of the Douglas-fir primer pairs also amplified SSR-containing loci in other conifer species.

scholarly journals Effect of Prunus serotina Ehrh. Volatile Compounds on Germination and Seedling Growth of Pinus sylvestris L.

Forests ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 846
Author(s):  
Aleksandra Halarewicz ◽  
Antoni Szumny ◽  
Paulina Bączek
Keyword(s):  
Pinus Sylvestris ◽  
Volatile Compounds ◽  
Scots Pine ◽  
Prunus Serotina ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Fraction ◽  
Black Cherry ◽  
Allelopathic Potential ◽  
Pine Seedlings ◽  
Phytotoxic Effect

In temperate European forests invaded by Prunus serotina Ehrh. (black cherry), a reduction in the spontaneous regeneration capacity of Pinus sylvestris L. (Scots pine) is observed. It could be caused by various factors, including allelopathic properties of this invasive plant. In this study the phytotoxic effect of P. serotina volatile compounds on P. sylvestris and the seasonal variation in this effect were assessed. Simple assays showed that volatiles emitted from P. serotina leaves significantly inhibited root growth of P. sylvestris seedlings. Their negative effect on stem growth was much weaker. The strongest phytotoxic effect on Scots pine seedlings was caused by the volatiles emitted from the youngest black cherry leaves. In fresh foliage of P. serotina, nineteen volatile organic compounds were identified by gas chromatography–mass spectrometry (GC–MS). The dominant compound was benzaldehyde. On the basis of tests of linalool alone, it was found that this monoterpene present in the volatile fraction has a strong allelopathic potential and inhibits germination, root elongation and shoot elongation of pine seedlings. The results of our research suggest that volatile compounds from P. serotina leaves could limited survival of P. sylvestris individuals in the seedling phase.

scholarly journals Location, but not defensive genotype, determines ectomycorrhizal community composition in Scots pine ( Pinus sylvestris L.) seedlings

2021 ◽  
Author(s):  
Jim Downie ◽  
Andy F. S. Taylor ◽  
Glenn Iason ◽  
Ben Moore ◽  
Jonathan Silvertown ◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Community Composition ◽  
Scots Pine ◽  
Ectomycorrhizal Community
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