Eukaryotic ribosomal RNA: the recent excitement in the nucleotide modification problem

Chromosoma ◽  
1997 ◽  
Vol 105 (7-8) ◽  
pp. 391-400 ◽  
Author(s):  
B. Edward H. Maden ◽  
John M. X. Hughes
Keyword(s):  
Ribosomal Rna ◽  
Nucleotide Modification

Eukaryotic ribosomal RNA: the recent excitement in the nucleotide modification problem

Chromosoma ◽  
1997 ◽  
Vol 105 (7-8) ◽  
pp. 391-400 ◽  
Author(s):  
B. Edward H. Maden ◽  
John M. X. Hughes
Keyword(s):  
Ribosomal Rna ◽  
Nucleotide Modification

Visualisation of E. coli ribosomal RNA in situ by electron spectroscopic imaging and image averaging

1992 ◽  
Vol 50 (1) ◽  
pp. 466-467
Author(s):  
Daniel Beniac ◽  
George Harauz
Keyword(s):  
Ribosomal Rna ◽  
Three Dimensional ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
E Coli ◽  
Microscopical Technique ◽  
Quantitative Image ◽  
Dimensional Reconstruction ◽  
Image Averaging ◽  
Protein Components

The structures of E. coli ribosomes have been extensively probed by electron microscopy of negatively stained and frozen hydrated preparations. Coupled with quantitative image analysis and three dimensional reconstruction, such approaches are worthwhile in defining size, shape, and quaternary organisation. The important question of how the nucleic acid and protein components are arranged with respect to each other remains difficult to answer, however. A microscopical technique that has been proposed to answer this query is electron spectroscopic imaging (ESI), in which scattered electrons with energy losses characteristic of inner shell ionisations are used to form specific elemental maps. Here, we report the use of image sorting and averaging techniques to determine the extent to which a phosphorus map of isolated ribosomal subunits can define the ribosomal RNA (rRNA) distribution within them.

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