Faculty Opinions recommendation of 18S ribosomal RNA and tetrapod phylogeny.

Author(s):  
Elizabeth Kellogg
Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1149
Author(s):  
Dina M. Metwally ◽  
Isra M. Al-Turaiki ◽  
Najwa Altwaijry ◽  
Samia Q. Alghamdi ◽  
Abdullah D. Alanazi

We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of Trypanosoma spp. in the camels. Trypanosoma evansi (T. evansi) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated T. evansi was closely related to the T. evansi that was detected in C. dromedarius in Egypt and the T. evansi strain B15.1 18S ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of T. evansi from beef cattle in Thailand and to T. brucei B8/18 18S ribosomal RNA from pigs in Nigeria.


GigaScience ◽  
2020 ◽  
Vol 9 (12) ◽  
Author(s):  
Haris Zafeiropoulos ◽  
Ha Quoc Viet ◽  
Katerina Vasileiadou ◽  
Antonis Potirakis ◽  
Christos Arvanitidis ◽  
...  

EMBO Reports ◽  
2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Jessica Leismann ◽  
Mariangela Spagnuolo ◽  
Mihika Pradhan ◽  
Ludivine Wacheul ◽  
Minh Anh Vu ◽  
...  

1993 ◽  
Vol 2 (2) ◽  
pp. 132-142 ◽  
Author(s):  
Dorothy P. Pashley ◽  
Bruce A. McPheron ◽  
Elizabeth A. Zimmer

Biochemistry ◽  
1988 ◽  
Vol 27 (2) ◽  
pp. 582-592 ◽  
Author(s):  
Asha Rairkar ◽  
Heidi M. Rubino ◽  
Raymond E. Lockard

2002 ◽  
Vol 23 (2) ◽  
pp. 288-292 ◽  
Author(s):  
Martı́n Garcı́a-Varela ◽  
Michael P Cummings ◽  
Gerardo Pérez-Ponce de León ◽  
Scott L Gardner ◽  
Juan P Laclette

Development ◽  
1974 ◽  
Vol 32 (2) ◽  
pp. 515-532
Author(s):  
A. Colman

RNA synthesis can be maintained in large oocytes of Xenopus laevis during periods of in vitro culture of at least 10 days. A simple salt medium, modified Barth's solution, is found to be as effective a culture medium for these oocytes as several other complex media. The newly synthesized RNA is characterized electrophoretically and shown to consist predominantly of ribosomal RNA precursor, 28S and 18S ribosomal RNA, and 4S RNA. The distribution of this RNA within the oocyte is detected autoradiographically, where it is found to be greatly concentrated over the nucleoli. No qualitative alterations in either of these parameters are found during culture, within the limits of sensitivity of the assay procedures.


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