Genomic dispersion of 28S rDNA during karyotypic evolution in the ant genusMyrmecia (formicidae)

Dipterocarpus dyeri is a typical plant of tropical evergreen moist forest at Southeast Vietnam. These plants have been planted popularly at parks and urban streets for the shade and it has been commonly materials for timber industry. Multiplication of Dipterocarpus dyeri at nurseries could face to some diseases, such as the withered disease cause serial death. Our study isolated three disease fungi strains from the root areas of the diseased Dipterocarpus dyeri planted Ma Da nursery, Dong Nai province. Result of 28s rDNA sequencing showed these fungi belong to Ophiostoma eucalypticagena, Aspergillus nidulans and Collectotrichum gloeosporioides. This result is base for conducting the following studies to control the withered disease on Dipterocarpus dyeri at the nursery.

Download Full-text

Molecular Characterisation and Phylogenetic Analysis of Dermatophytic Fungi Isolated from Tinea Capitis in Northwest Nigeria Using Sequence of the 28S rRNA

Microbiology Research ◽

10.3390/microbiolres12030046 ◽

2021 ◽

Vol 12 (3) ◽

pp. 646-655

Author(s):

Hussain Yahaya Ungo-kore ◽

Joseph Olorunmola Ehinmidu ◽

Josiah Ademola Onaolapo ◽

Olayeni Stephen Olonitola

Keyword(s):

Phylogenetic Analysis ◽

Tinea Capitis ◽

28S Rdna ◽

Clinical Samples ◽

28S Rrna ◽

Trichophyton Tonsurans ◽

Detection And Identification ◽

Microsporum Audouinii ◽

Trichophyton Simii ◽

Dermatophyte Species

The detection and identification of fungal DNA from clinical samples is one of the fundamental approaches in biomedicine. The incidence, distribution, and control of dermatophytes has progress significantly and the use of phylogenetic species concepts based on rRNA regions have enhanced the taxonomy of dermatophyte species; however, the use of 28S rDNA genes has certain limitations. This gene has been used in dermatophyte taxonomy with limited enumeration; we appraised the sequence disparity within and among groups of the species, the gene ranking in identification, phylogenetic analysis, and taxonomy of 32 strains of eight dermatophyte species. In this study, a set of primers was adopted to amplify the target followed by a partial sequencing of the rDNA. The utilization of a pairwise nucleotide differentiation, an affinity was observed among eight dermatophyte species, with disparity among species ranging from 0 to 197 base pair (bp). Intra-species bp differences were found within strains of Trichophyton eriotrephon, Trichophyton bullosum, Trichophyton simii (Trichophyton genus), Microsporum audouinii, and Trichophyton tonsurans (Microsporum and Trichophyton genus, respectively); however, only some strains of Trichophyton eriotrephon were found to be invariant having three genotypes. Trichophyton tonsurans exhibited most intra-species variability. The characterization and construction of a phylogenetic tree of 28S rDNA gene on dermatophyte species provide a bedrock of an additional finding of connections between species. However, 28S rRNA capture provides a novel method of effective and sensitive detection of dermatophytes lodged in human skin scale. We report for the first time the emergence of T. eriotrephon, T. bullosum, T. simii, T. benhamiae, and Ctenomyces serratus dermatophytes from Tinea capitis in Nigeria.

Download Full-text

Interstitial localization of telomeric DNA sequences in the Indian muntjac chromosomes: further evidence for tandem chromosome fusions in the karyotypic evolution of the Asian muntjacs

Cytogenetic and Genome Research ◽

10.1159/000133525 ◽

1993 ◽

Vol 63 (3) ◽

pp. 156-159 ◽

Cited By ~ 62

Author(s):

C. Lee ◽

R. Sasi ◽

C.C. Lin

Keyword(s):

Dna Sequences ◽

Karyotypic Evolution ◽

Telomeric Dna ◽

Indian Muntjac ◽

Chromosome Fusions

Download Full-text

Detection of mucormycetes and other pathogenic fungi in formalin fixed paraffin embedded and fresh tissues using the extended region of 28S rDNA

Medical Mycology ◽

10.1093/mmy/myw083 ◽

2016 ◽

pp. myw083 ◽

Cited By ~ 2

Author(s):

Lalitha Gade ◽

Steven Hurst ◽

S. Arunmozhi Balajee ◽

Shawn R. Lockhart ◽

Anastasia P. Litvintseva

Keyword(s):

Pathogenic Fungi ◽

28S Rdna ◽

Extended Region ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

Download Full-text

Multiplex PCR assay and phylogenetic analysis of sequences derived from D2 domain of 28S rDNA distinguished members of the Anopheles culicifacies complex into two groups, A/D and B/C/E

Infection Genetics and Evolution ◽

10.1016/j.meegid.2008.12.007 ◽

2009 ◽

Vol 9 (2) ◽

pp. 271-277 ◽

Cited By ~ 10

Author(s):

K. Raghavendra ◽

Anthony J. Cornel ◽

B.P. Niranjan Reddy ◽

Frank H. Collins ◽

Nutan Nanda ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Multiplex Pcr ◽

28S Rdna ◽

Anopheles Culicifacies ◽

Pcr Assay ◽

Multiplex Pcr Assay ◽

D2 Domain

Download Full-text

Diversity and Karyotypic Evolution in the Genus Neacomys (Rodentia, Sigmodontinae)

Cytogenetic and Genome Research ◽

10.1159/000441173 ◽

2015 ◽

Vol 146 (4) ◽

pp. 296-305 ◽

Cited By ~ 9

Author(s):

Willam O. da Silva ◽

Julio C. Pieczarka ◽

Rogério V. Rossi ◽

Horacio Schneider ◽

Iracilda Sampaio ◽

...

Keyword(s):

Molecular Phylogeny ◽

Diploid Number ◽

Constitutive Heterochromatin ◽

Chromosomal Evolution ◽

The Other ◽

Karyotypic Evolution ◽

Drastic Reduction ◽

Ancestral Species ◽

Pericentric Inversions ◽

Amazonian Region

Neacomys (Sigmodontinae) comprises 8 species mainly found in the Amazonian region. We describe 5 new karyotypes from Brazilian Amazonia: 2 cytotypes for N. paracou (2n = 56/FNa = 62-66), 1 for N. dubosti (2n = 64/FNa = 68), and 2 for Neacomys sp. (2n = 58/FNa = 64-70), with differences in the 18S rDNA. Telomeric probes did not show ITS. We provide a phylogeny using Cytb, and the analysis suggests that 2n = 56 with a high FNa is ancestral for the genus, as found in N. paracou, being retained by the ancestral forms of the other species, with an increase in 2n occurring independently in N. spinosus and N. dubosti. Alternatively, an increase in 2n may have occurred in the ancestral taxon of the other species, followed by independent 2n-reduction events in Neacomys sp. and in the ancestral species of N. tenuipes, N. guianae, N. musseri, and N. minutus. Finally, a drastic reduction event in the diploid number occurred in the ancestral species of N. musseri and N. minutus which exhibit the lowest 2n of the genus. The karyotypic variations found in both intra- and interspecific samples, associated with the molecular phylogeny, suggest a chromosomal evolution with amplification/deletion of constitutive heterochromatin and rearrangements including fusions, fissions, and pericentric inversions.

Download Full-text