Multiplex PCR assay and phylogenetic analysis of sequences derived from D2 domain of 28S rDNA distinguished members of the Anopheles culicifacies complex into two groups, A/D and B/C/E

2009 ◽  
Vol 9 (2) ◽  
pp. 271-277 ◽  
Author(s):  
K. Raghavendra ◽  
Anthony J. Cornel ◽  
B.P. Niranjan Reddy ◽  
Frank H. Collins ◽  
Nutan Nanda ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Multiplex Pcr ◽  
28S Rdna ◽  
Anopheles Culicifacies ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
D2 Domain

scholarly journals Lactobacillus plantarum subsp. argentoratensis subsp. nov., isolated from vegetable matrices

2005 ◽  
Vol 55 (4) ◽  
pp. 1629-1634 ◽  
Author(s):  
Françoise Bringel ◽  
Anna Castioni ◽  
Daniel K. Olukoya ◽  
Giovanna E. Felis ◽  
Sandra Torriani ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Multiplex Pcr ◽  
Lactobacillus Plantarum ◽  
Type Strain ◽  
Homogeneous Group ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Protein Encoding ◽  
Species Specific ◽  
Encoding Genes

Fourteen strains isolated from vegetable sources and identified as belonging to Lactobacillus plantarum presented an atypical pattern of amplification with a species-specific multiplex-PCR assay. Phylogenetic analysis of two protein-encoding genes, recA (encoding the recombinase A protein) and cpn60 (encoding the GroEL chaperonin), as well as phenotypic and genomic traits revealed a homogeneous group of very closely related strains for which subspecies status is proposed, with the name Lactobacillus plantarum subsp. argentoratensis. The type strain is DKO 22T (=CIP 108320T=DSM 16365T).

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

New approach for the identification of potentially toxigenic Corynebacterium sp. using a multiplex PCR assay

2021 ◽  
pp. 106198
Author(s):  
Sunarno ◽  
Khariri ◽  
Fauzul Muna ◽  
Kambang Sariadji ◽  
Yuni Rukminiati ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Pcr Assay ◽  
New Approach ◽  
Multiplex Pcr Assay ◽  
Corynebacterium Sp

A multiplex PCR assay to diagnose and quantify Nosema infections in honey bees (Apis mellifera)

2010 ◽  
Vol 105 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Mollah Md. Hamiduzzaman ◽  
Ernesto Guzman-Novoa ◽  
Paul H. Goodwin
Keyword(s):  
Apis Mellifera ◽  
Multiplex Pcr ◽  
Honey Bees ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Development of a Multiplex PCR Assay to Detect Gastroenteric Pathogens in the Feces of Mexican Children

2012 ◽  
Vol 65 (4) ◽  
pp. 361-368 ◽  
Author(s):  
R. Tolentino-Ruiz ◽  
D. Montoya-Varela ◽  
M. García-Espitia ◽  
M. Salas-Benito ◽  
A. Gutiérrez-Escolano ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Pcr Assay ◽  
Mexican Children ◽  
Multiplex Pcr Assay
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