Optimization of in vitro bud induction and plantlet formation from mature embryos of Aleppo pine (Pinus halepensis Mill.)

1993 ◽  
Vol 29 (4) ◽  
pp. 189-199 ◽  
Author(s):  
Maurizio Lambardi ◽  
Kiran K. Sharma ◽  
Trevor A. Thorpe
Keyword(s):  
Pinus Halepensis ◽  
Aleppo Pine ◽  
Mature Embryos ◽  
Plantlet Formation ◽  
Bud Induction

IN VITRO REGENERATION OF SHOOT BUDS AND PLANTLET FORMATION FROM MATURE EMBRYOS OF PINUS HALEPENSIS MILL.

1991 ◽  
pp. 123-124 ◽  
Author(s):  
M. Lambardi ◽  
K.K. Sharma ◽  
T.A. Thorpe
Keyword(s):  
Pinus Halepensis ◽  
In Vitro Regeneration ◽  
Shoot Buds ◽  
Mature Embryos ◽  
Plantlet Formation

scholarly journals Induction of shoot buds, multiplication and plantlet formation in seedling explants of bell pepper (Capsicum annuum L.) cv. Bryza in vitro

2014 ◽  
Vol 71 (3) ◽  
pp. 187-193
Author(s):  
Andrzej Gatz
Keyword(s):  
Capsicum Annuum ◽  
Cotyledonary Node ◽  
Explant Culture ◽  
Shoot Tip ◽  
Shoot Buds ◽  
Plantlet Formation ◽  
Shoot Bud ◽  
Cotyledon Explants ◽  
Bud Induction

In vitro shoot bud induction and multiplication as well as plantlets formation from different parts of 21-d old seedlings (shoot tip, cotyledonary node, distal part of cotyledon, acropetal section of hypocotyl) of <em>Capsicum annuum</em> L., cv. Bryza were compared. During 4 weeks of primary explant culture on initiation media, first shoot bud primordia appeared; they reminded leaf primordia and subsequently some of them underwent enlargement, some developed into leaves and leaf-like structures (mainly on cotyledon explants). The highest number of shoot bud primordia was noted on cotyledonary node explants, but they were smaller than those on the remaining types of the explants. The best response of shoot regeneration showed cotyledon explants on which most of shoot buds were formed in each from four treated passages. From shoot buds on elongation media after 4 weeks of culture rooted rosettes of leaves were achieved, and the extension of the culture time to eight weeks without subculture caused that the rosettes developed into plantlets. Throughout four successive passages plantlets were obtained from cotyledon and shoot tip explants.

Tree ring to climate relationships of Aleppo pine (Pinus halepensis Mill.) in Greece

2001 ◽  
Vol 27 (1) ◽  
pp. 89-98 ◽  
Author(s):  
Andreas Papadopoulos ◽  
Françoise Serre-Bachet ◽  
Lucien Tessier
Keyword(s):  
Tree Ring ◽  
Pinus Halepensis ◽  
Aleppo Pine

Organogenesis and Plantlet formation in vitro in Dalbergia sissoo Roxb.

1988 ◽  
Vol 132 (2) ◽  
pp. 145-147 ◽  
Author(s):  
Sudha Sharma ◽  
N. Chandra
Keyword(s):  
Dalbergia Sissoo ◽  
Plantlet Formation

scholarly journals In vitro shoot regeneration of boldo from leaf explants

2010 ◽  
Vol 40 (10) ◽  
pp. 2210-2213
Author(s):  
Monalize Salete Mota ◽  
Juliana de Magalhães Bandeira ◽  
Eugenia Jacira Bolacel Braga ◽  
Valmor João Bianchi ◽  
José Antonio Peters
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Shoot Development ◽  
High Potential ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Bud Induction ◽  
Molecular Studies ◽  
In Vitro Shoot Regeneration

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

Interazioni in Vitro TraSuillus Granulatus(L.) Kuntze e Miceli Sterili Demaziacei Associati ad Ectomicorrize di Pinus Halepensis Mill

1996 ◽  
Vol 130 (1) ◽  
pp. 283-283
Author(s):  
M. Girlanda ◽  
S. Buonomo ◽  
A. M. Luppi-Mosca
Keyword(s):  
Pinus Halepensis

Disease-resistant transgenic adzuki bean plants obtained through an efficient transformation system

2012 ◽  
Vol 63 (12) ◽  
pp. 1090 ◽  
Author(s):  
Huatao Chen ◽  
Xin Chen ◽  
Heping Gu ◽  
Xingxing Yuan ◽  
Hongmei Zhang ◽  
...  
Keyword(s):  
Soybean Mosaic Virus ◽  
Shoot Elongation ◽  
Zeatin Riboside ◽  
Medium Composition ◽  
Adventitious Bud ◽  
Adzuki Bean ◽  
Transformation System ◽  
Bean Plants ◽  
Bud Induction

An efficient regeneration and transformation system was established and optimised for adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi). 6-Benzylaminopurine at 5 mg L–1 was used to increase adventitious bud induction frequency. The highest frequency of shoot elongation was 92.8% when using a medium composition of MS salts combined with 0.1 mg L–1 of IAA, 0.5 mg L–1 of GA3, 1.0 mg L–1 of zeatin-riboside, 50 mg L–1 of aspartic acid, and 50 mg L–1 of glutamic acid. In vitro rooting was 100% when shoots were cultured on the solid MS medium supplemented with 1.0 mg L–1 of NAA. Reproducible transformation of epicotyl explants was developed using the A. tumefaciens EHA105 strain. Using a concentration of 40 mg L–1 of acetosyringone, 20 mm MES, and 5 mg L–1 of 6-benzylaminopurine in the co-cultivation medium, a transformation efficiency of 12.6% was attained. Using this transformation protocol, we obtained transgenic adzuki bean plants resistant to soybean mosaic virus by introducing the V. angularis VaPR3 gene.

Frost hardening and resistance in three Aleppo pine (Pinus halepensis Mill.) provenances

2001 ◽  
Vol 49 (3) ◽  
pp. 179-186
Author(s):  
ELENA PAOLETTI ◽  
ROBERTO CALAMASSI ◽  
SARA STRATI
Keyword(s):  
Pinus Halepensis ◽  
Aleppo Pine ◽  
Frost Hardening

scholarly journals Structural variability of Aleppo pine stands in two forests in northeastern Algeria

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
Malika Rached Kanouni ◽  
Insaf Hani ◽  
Ratiba BOUSBA ◽  
Amina Beldjazia ◽  
Hichem KHAMAR
Keyword(s):  
Pinus Halepensis ◽  
Small Diameter ◽  
Basal Area ◽  
Aleppo Pine ◽  
Regeneration Rate ◽  
Tree Diameter ◽  
Total Height ◽  
Structural Variability ◽  
Pine Stands ◽  
Northeastern Algeria

Abstract. Rached-Kanouni M, Hani I, Bousba R, Beldjazia A, Khammar H. 2020. Structural variability of Aleppo pine stands in two forests in northeastern Algeria. Biodiversitas 21: 2848-2853. The layout of the stand can be described as the width of the trees, their reciprocal locations, diametric distinction and height. The goal of this study is to recognize changes in the Pinus halepensis spatial and demographic systems in two Beni Oudjana and Chettaba Forests, located in northeast Algeria. An inventory of trees in these forest formations with P. halepensis dominance was carried out based on dendrometric parameters such as total height, tree diameter at dbh ≥ 5 cm, basal area, total volume, etc., as well as the number of trees in the forest. Tree diameter and height measurements were made on 12 rectangular plots (20 m × 20 m), located in both forests. The results obtained show that the mean stand density, mean diameter, basal area and total volume are higher in Chettaba Forest, the values attributed to these parameters are respectively (422 trees/ha, 27.07 cm, 26, 86 m2, 251.63 m3); while the total height and regeneration rate show significantly higher values in Beni Oudjana Forest (18.97 m, 607 individuals/ha). The structure in diameter and height of the species is bell-shaped to asymmetrically positive with a predominance of small diameter individuals in the Chettaba forest. On the other hand, in the Beni Oudjana Forest, the structure is ‘L’ shaped, showing a predominance of very small diameter individuals. These results indicate that the low regeneration rate of P. halepensis in the Chettaba Forest is due to anthropogenic pressures that favor the degradation of this forest.

Sign in / Sign up

Export Citation Format

Share Document