Genetic Improvement in Pine Wilt Disease Resistance in Pinus thunbergii: The Effectiveness of Pre-screening with an Artificial Inoculation at the Nursery

2001 ◽  
Vol 6 (3) ◽  
pp. 197-201 ◽  
Author(s):  
Tadao Toda ◽  
Susumu Kurinobu
Keyword(s):  
Disease Resistance ◽  
Genetic Improvement ◽  
Pine Wilt Disease ◽  
Wilt Disease ◽  
Pinus Thunbergii ◽  
Artificial Inoculation ◽  
Pine Wilt

Spatiotemporal analysis of pine wilt disease: Relationship between pinewood nematode distribution and defence response in Pinus thunbergii seedlings

2019 ◽  
Vol 49 (4) ◽  
pp. e12518 ◽  
Author(s):  
Rimi Yamaguchi ◽  
Koji Matsunaga ◽  
Tomonori Hirao ◽  
Miho Tamura ◽  
Atsushi Watanabe
Keyword(s):  
Pine Wilt Disease ◽  
Spatiotemporal Analysis ◽  
Wilt Disease ◽  
Pinus Thunbergii ◽  
Defence Response ◽  
Pinewood Nematode ◽  
Pine Wilt

Endogenous bacterial community structure of Pinus massoniana with differing resistance to pine wilt disease

Nematology ◽  
2021 ◽  
pp. 1-17
Author(s):  
Wei Lu ◽  
Xiao-Jia Zhao ◽  
Jia-Jin Tan
Keyword(s):  
Disease Resistance ◽  
Bacterial Community ◽  
Endophytic Bacteria ◽  
Pine Wilt Disease ◽  
Illumina Miseq ◽  
Pinus Massoniana ◽  
Wilt Disease ◽  
Pine Wilt ◽  
Disease Resistant ◽  
Endophytic Bacterial Community

Summary Pine wilt disease (PWD) is a devastating pine disease caused by Bursaphelenchus xylophilus and its main host in China is Pinus massoniana. The relationship between endophytic bacteria and disease resistance in P. massoniana remains unclear. In this paper, the leaves, roots, stems and treetops of different disease-resistant P. massoniana were studied as the research objective and Illumina MiSeq sequencing was used to analyse whether there were significant differences in the composition and diversity of endophytic bacterial communities between different disease-resistant P. massoniana. The results showed that at the genus level there were no obvious differences in the composition of the endophytic bacterial community of different disease-resistant P. massoniana in the leaves, but there were obvious differences in the roots, stems and treetops. The richness and diversity of endophytic bacteria in P. massoniana had no significant impact on its disease resistance, whilst the structure of endophytic bacterial community in stems and treetops may be related to its disease resistance.

Influence of temperature on pine wilt disease progression in Pinus thunbergii seedlings

2019 ◽  
Vol 156 (2) ◽  
pp. 581-590 ◽  
Author(s):  
R. Yamaguchi ◽  
K. Matsunaga ◽  
A. Watanabe
Keyword(s):  
Disease Progression ◽  
Pine Wilt Disease ◽  
Wilt Disease ◽  
Pinus Thunbergii ◽  
Influence Of Temperature ◽  
Pine Wilt ◽  
Influence Of Temperature On

Role of resin canals in the early stages of pine wilt disease caused by the pine wood nematode

1997 ◽  
Vol 75 (2) ◽  
pp. 346-351 ◽  
Author(s):  
Kyoko Ishida ◽  
Taizo Hogetsu
Keyword(s):  
Pine Wilt Disease ◽  
Wilt Disease ◽  
Pinus Thunbergii ◽  
Pine Wood Nematode ◽  
Pine Wood ◽  
Pine Wilt ◽  
Bark Peeling ◽  
Resin Canals ◽  
Cut Surface

The role of cortical resin canals in the early development of a pine wilt disease in Japanese black pine, Pinus thunbergii, was studied. A part of the bark of a 2 cm long segment from a current-year stem was removed by a tangential cut with a razor blade. Both cortex-exposed segments with cut cortical resin canals (designated as +RC segments) and those without cut resin canals (–RC segments) were obtained by chance. When a virulent nematode isolate (S6-1) was inoculated onto the cut surface, the surface of the +RC segments turned brown 4 d after inoculation, and in some segments this browning occurred more intensely around cortical resin canals. When segments were cut transversely at the middle, the transverse cut surface of the inoculated +RC segments was brown and fragile, but that of the inoculated –RC segments was pale green and stable, as was that of the non-inoculated controls. Correspondingly, tissue cells including epithelial cells of the cortical resin canal of the +RC segments were all dead, but those of the –RC segments and the controls were alive. When nematodes were inoculated onto the inner surface (cambium side) of a bark peeling at which cambial xylem cells were exposed, they did not kill the cambial cells. When inoculated on the transverse cut end of a bark peeling from which nematodes would enter resin canals, nematodes killed all cells in the peeling. The above results indicate that nematodes do not kill cortical cells immediately after inoculation but become harmful to pine cells after living in cortical resin canals. Key words: Bursaphelenchus xylophilus, pine wilt disease, pine wood nematode, Pinus thunbergii, resin canal.

scholarly journals Usefulness of the Closed Cutting System for the Vegetative Propagation of Pinus thunbergii Resistant to Pine Wilt Disease.

2009 ◽  
Vol 91 (4) ◽  
pp. 266-276 ◽  
Author(s):  
Mineko Ohira ◽  
Noritsugu Kuramoto ◽  
Yoshitake Fujisawa ◽  
Susumu Shiraishi
Keyword(s):  
Vegetative Propagation ◽  
Pine Wilt Disease ◽  
Wilt Disease ◽  
Pinus Thunbergii ◽  
Pine Wilt ◽  
Cutting System

Distribution and pathogenicity of bacteria species carried by Bursaphelenchus xylophilus in China

Nematology ◽  
2003 ◽  
Vol 5 (6) ◽  
pp. 899-906 ◽  
Author(s):  
Bo Guang Zhao ◽  
Hu Li Wang ◽  
Su Fen Han ◽  
Zheng Ming Han
Keyword(s):  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Pinus Thunbergii ◽  
Automatic Testing ◽  
Pine Wood Nematode ◽  
Field Inoculation ◽  
Pine Wood ◽  
Pine Wilt

Abstract Bacteria carried by the pine wood nematode (PWN), Bursaphelenchus xylophilus, from both healthy and diseased Pinus thunbergii and P. massoniana were studied in five main pine wilt disease epidemic provinces in P. R. China. No bacteria were found in healthy pines but were found on PWN from all samples collected from diseased trees. Twenty-four bacteria strains were isolated from the nematodes and were identified by a combination of classical and automatic testing bacteriology (ATB) expression methods. Bioassay showed that 17 of the 24 identified strains produced phytotoxins. Eleven of these 17 phytotoxin producers belonged to the genus Pseudomonas. Glasshouse and field inoculation tests using sterile techniques showed that both PWN and the toxin-producing bacteria carried were necessary to induce disease. We hypothesise that pine wilt disease is a complex, induced by both PWN and the bacteria it carries.

scholarly journals Tissue Degeneration in Japanese Black Pine (Pinus thunbergii) Mother Trees and Seedlings that are Resistant to Pine Wilt Disease.

2010 ◽  
Vol 92 (1) ◽  
pp. 16-21
Author(s):  
Tetsuji Hakamata ◽  
Kimihiko Kato ◽  
Shigehiro Yamamoto
Keyword(s):  
Pine Wilt Disease ◽  
Wilt Disease ◽  
Pinus Thunbergii ◽  
Black Pine ◽  
Tissue Degeneration ◽  
Pine Wilt ◽  
Japanese Black Pine
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