Importance of growth form on production of hybrid antibiotic byStreptomyces lividans TK21 by fed-batch and continuous fermentation

1998 ◽  
Vol 75 (2-3) ◽  
pp. 235-248 ◽  
Author(s):  
Montserrat Sarrà ◽  
Josep A. Pérez-Pons ◽  
Francesc Gòdia ◽  
Carlos Casas Alvero
Keyword(s):  
Growth Form ◽  
Continuous Fermentation ◽  
Fed Batch ◽  
Hybrid Antibiotic

Optimized nikkomycin production by fed-batch and continuous fermentation

1993 ◽  
Vol 39 (4-5) ◽  
pp. 433-437 ◽  
Author(s):  
Traugott C. Sch�z ◽  
Hans-Peter Fiedler ◽  
Hans Z�hner
Keyword(s):  
Continuous Fermentation ◽  
Fed Batch

Fed-batch and continuous fermentation ofSelenomonas ruminantium for natural propionic, acetic and succinic acids

1995 ◽  
Vol 15 (1) ◽  
pp. 32-38 ◽  
Author(s):  
D C Eaton ◽  
A Gabelman
Keyword(s):  
Continuous Fermentation ◽  
Fed Batch

Enhanced phenylpyruvic acid production with Proteus vulgaris in fed-batch and continuous fermentation

2016 ◽  
Vol 46 (2) ◽  
pp. 157-160 ◽  
Author(s):  
Hasan B. Coban ◽  
Ali Demirci ◽  
Paul H. Patterson ◽  
Ryan J. Elias
Keyword(s):  
Acid Production ◽  
Continuous Fermentation ◽  
Proteus Vulgaris ◽  
Fed Batch ◽  
Phenylpyruvic Acid

scholarly journals Implementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by Methanol Rate

2021 ◽  
Vol 11 (5) ◽  
pp. 12633-12641
Keyword(s):  
Pichia Pastoris ◽  
Cell Density ◽  
Batch Fermentation ◽  
Fermentation Process ◽  
Production Efficiency ◽  
Continuous Fermentation ◽  
Scale Up ◽  
Fed Batch ◽  
Simple Method ◽  
Fed Batch Fermentation

High cell density fed-batch fermentation is the main strategy for recombinant hepatitis B surface antigen (rHBsAg) production. In this study, we employed short-term continuous fermentation to optimize the cell density of recombinant Pichia pastoris (P. pastoris). After reaching the maximum specified broth volume of 5 L in the fed-batch fermentation process, the operation mode was altered into the continuous mode with a dilution rate of 0.009 1/h. We used various values of methanol inflow to examine its impact as a limiting nutrient on cell density. After reaching the steady-state point, the continuous fermentation was stopped. The process's performance was evaluated based on titer, yield, productivity, and ease of process control. According to the results, the optimal methanol inflow in the pilot-scale fermentation process was 39.9 ml/h as the cell density increased from 363 g/l wet cell weight (WCW) in the fed-batch stage to 450 g/l WCW. We could successfully scale up the fermentation process with the biomass concentration of 450 g/l without having any major issues such as excessive heat dissipation or insufficient oxygen supply. This approach is a simple method for enhancing rHBsAg production efficiency in P. pastoris without requiring any new and complex facility.

scholarly journals A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

2018 ◽  
Author(s):  
Martin Koller
Keyword(s):  
Continuous Fermentation ◽  
Feeding Strategy ◽  
Operation Mode ◽  
Microbial Production ◽  
Batch Processes ◽  
Gas Transfer ◽  
Fed Batch ◽  
Production Processes ◽  
Multi Stage ◽  
Pha Production

Polyhydroxyalkanoates (PHA) are microbial biopolyesters utilized as “green plastics”. Their production under controlled conditions resorts to bioreactors operated in different modes. Because PHA biosynthesis constitutes a multiphase process, both feeding strategy and bioreactor operation mode need smart adaptation. Traditional PHA production setups based on batch, repeated batch, fed-batch or cyclic fed-batch processes are often limited in productivity, or display insufficient controllability of polyester composition. For highly diluted substrate streams like it is the case for (agro)industrial waste streams, fed-batch enhanced by cell recycling were recently reported as a viable tool to increase volumetric productivity. As emerging trend, continuous fermentation processes in single-, two-, and multi-stage setups are reported, which bring the kinetics of both microbial growth and PHA accumulation into agreement with process engineering, and allow tailoring PHA´s molecular structure. Moreover, we currently witness an increasing number of CO2-based PHA production processes using cyanobacteria; these light-driven processes resort to photobioreactors similar to those used for microalgae cultivation, and can be operated both discontinuously and continuously. This development goes in parallel to the emerging use of methane and syngas as an abundantly available gaseous substrates, which also calls for bioreactor systems with optimized gas transfer. The review sheds light on the challenges of diverse PHA production processes in different bioreactor types and operational regimes using miscellaneous microbial production strains such as extremophilic Archaea, chemoheterotrophic eubacteria and phototrophic cyanobacteria. Particular emphasize is dedicated to the limitations and promises of different bioreactor-strain combinations, and to efforts devoted to upscaling these processes to industrially relevant scales.

Importance of spore mutants for fed-batch and continuous fermentation ofBacillus subtilis

1995 ◽  
Vol 47 (6) ◽  
pp. 696-702 ◽  
Author(s):  
M. K. Oh ◽  
B. G. Kim ◽  
S. H. Park
Keyword(s):  
Continuous Fermentation ◽  
Fed Batch ◽  
Ofbacillus Subtilis

scholarly journals Comparative Study for the Production of Mycophenolic Acid using Penicillium brevicompactum in Batch, Fed-batch and Continuous Fermentation Process

2021 ◽  
Vol 12 (1) ◽  
pp. 366-376
Keyword(s):  
Mycophenolic Acid ◽  
Acid Production ◽  
Batch Fermentation ◽  
Fermentation Process ◽  
Continuous Fermentation ◽  
Operating Conditions ◽  
Submerged Cultivation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Penicillium Brevicompactum

Mycophenolic acid (MPA) is, due to its immunosuppressive and biological activities, a potential compound. It is the secondary metabolite produced in submerged cultivation by the microfungus Penicillium brevicompactum. Batch, fed-batch, and continuous mode of cultivation for mycophenolic acid production were performed and compared in the current work. To increase productivity, mycophenolic acid production was studied in batch, fed-batch, and continuous bioreactors. These experiments were conducted with a 2.5 L working volume in a 3.7 L continuous stirred tank bioreactor. In all cases, operating conditions such as temperature, pH, agitation, and aeration, 28 °C, 5.5, 200 rpm, and 2 vvm, respectively, were the same. In fed-batch fermentation, the MPA concentration obtained was 1.91 g/L higher than the value obtained in batch culture, 1.55 g/L, while in continuous fermentation, 1.67 g/L was obtained. The mycophenolic acid productivity obtained in the continuous fermentation process was 0.025 g/L/h, which was maximum MPA productivity, compared to 0.007 g/L/h in the fed-batch fermentation process and 0.006 g/L/h in the batch fermentation process. The impact of substrate inhibition on the product formation can effectively bring down by continuous fermentation processes. The MPA productivity was increased in continuous fermentation relative to batch and fed-batch processing. The finding indicates that continuous culture of Penicillium brevicompactum is a promising strategy for the synthesis of mycophenolic acid.

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