Theory of protein-induced lateral phase separation in lipid membranes

1989 ◽  
Vol 14 (1) ◽  
pp. 79-95 ◽  
Author(s):  
Maria M. Sperotto ◽  
John Hjort Ipsen ◽  
Ole G. Mouritsen
Keyword(s):  
Phase Separation ◽  
Lipid Membranes ◽  
Lateral Phase Separation

Ionotropic lateral phase separation in mixed lipid membranes: a theoretical study

1987 ◽  
Vol 91 (24) ◽  
pp. 6252-6257 ◽  
Author(s):  
Antonio. Raudino ◽  
Felice. Zuccarello ◽  
Giuseppe. Buemi
Keyword(s):  
Phase Separation ◽  
Lipid Membranes ◽  
Theoretical Study ◽  
Lateral Phase Separation ◽  
Mixed Lipid Membranes

scholarly journals Adhesion-induced lateral phase separation in membranes

2000 ◽  
Vol 3 (3) ◽  
pp. 259-271 ◽  
Author(s):  
S. Komura ◽  
D. Andelman
Keyword(s):  
Phase Separation ◽  
Lateral Phase Separation

Ca2+-induced phase separation in black lipid membranes and its effect on the transport of a hydrophobic ion

1985 ◽  
Vol 813 (2) ◽  
pp. 221-229 ◽  
Author(s):  
Alfred Miller ◽  
Günther Schmidt ◽  
Hansjörg Eibl ◽  
Wolfgang Knoll
Keyword(s):  
Phase Separation ◽  
Lipid Membranes ◽  
Black Lipid Membranes

scholarly journals Dynamics of Phase Separation in Lipid Membranes

2010 ◽  
Vol 98 (3) ◽  
pp. 664a
Author(s):  
Brian Camley ◽  
Frank L.H. Brown
Keyword(s):  
Phase Separation ◽  
Lipid Membranes

Chemically Driven Phase Separation in Black Lipid Membranes

1990 ◽  
pp. 241-248
Author(s):  
S. Mittler-Neher ◽  
J. Spinke ◽  
W. Knoll
Keyword(s):  
Phase Separation ◽  
Lipid Membranes ◽  
Black Lipid Membranes

scholarly journals Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas

ACS Nano ◽  
2017 ◽  
Vol 11 (7) ◽  
pp. 7241-7250 ◽  
Author(s):  
Pamina M. Winkler ◽  
Raju Regmi ◽  
Valentin Flauraud ◽  
Jürgen Brugger ◽  
Hervé Rigneault ◽  
...  
Keyword(s):  
Phase Separation ◽  
Lipid Membranes

scholarly journals Phase Diagram of Purified CNS Myelin Reveals Continuous Transformation between Expanded and Compacted Lamellar States

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 670
Author(s):  
Julio M. Pusterla ◽  
Emanuel Schneck ◽  
Rafael G. Oliveira
Keyword(s):  
Phase Diagram ◽  
Phase Separation ◽  
Isothermal Transformation ◽  
Phase Behaviour ◽  
Apparent Difference ◽  
X Ray Diffraction ◽  
Lateral Phase Separation ◽  
Diffraction Peaks ◽  
Low Ionic Strength ◽  
Detergent Resistant Membrane

Purified myelin membranes (PMMs) are the starting material for biochemical studies, from individual components up to the isolation of detergent-resistant membrane (DRM) fractions or detergent-insoluble glycosphingolipid (DIG) fractions, which are commonly believed to resemble physiological lipid rafts. The normal DIG isolation protocol involves the extraction of lipids under moderate cooling. The isolation of PMMs also involves the cooling of myelin as well as exposure to low ionic strength (IS). Here, we addressed the combined influence of cooling and IS on the structure of PMMs. The phase behaviour was investigated by small angle X-ray diffraction. Analysis of the diffraction peaks revealed the lamellar periodicity ( d ), the number of periodically correlated bilayers ( N ), and the relatives fractions of each phase. Departure from physiological conditions induced a phase separation in myelin. The effect of monovalent and divalent ions was also compared at equivalent IS, showing a differential effect, and phase diagrams for both ion types were established—Ca2+ induced the well-known over-compacted phase, but additionally we also found an expanded phase at low IS. Na+ promoted phase separation, and also induced over-compaction at sufficiently high IS. Finally, exploring the whole phase diagram, we found evidence for the direct isothermal transformation from the expanded to the compacted phase, suggesting that both phases could in fact originate from the identical primary lateral phase separation, whereas the apparent difference lies in the inter-bilayer interaction that is modulated by the ionic milieu.

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