Simplified single radial immunodiffusion detection of albumin receptors on HBsAG particles and its significance regarding sero-changes of hbeag in hepatitis-B patients

1984 ◽  
Vol 19 (2) ◽  
pp. 116-120 ◽  
Author(s):  
Takao Tsuji ◽  
Kenji Takahashi ◽  
Kunihiko Naito ◽  
Hideo Nagashima
1986 ◽  
Vol 161 (3) ◽  
pp. 275-282 ◽  
Author(s):  
Koji Itakura ◽  
Takeyasu Matsudate ◽  
Takato Sakurai ◽  
Shunichi Hashimoto ◽  
Kazue Ito ◽  
...  

1976 ◽  
Vol 22 (2) ◽  
pp. 267-269 ◽  
Author(s):  
A O Vladutiu ◽  
B M Winiarski

Abstract Quantitation of the C3 component of complement by single radial immunodiffusion is subject to error because the C3 molecule has several antigenic determinants and anti-C3 sera differ in their specificity to these determinants. C3 was measured in plasma (ethylenediaminetetraacetate anticoagulant) and serum, in five normal individuals, by use of four such commercial kits. The effects of ethylenediaminetetraacetate and of incubation at -20, 4, and 37 degrees C for 1, 2, and 7 days were investigated. We found wide variations in C3 in the same sample, as measured with different kits. Incubation for longer than 48 h at 4 degrees C changed C3 concentrations as compared to those in fresh samples. Reference sera for C3 assay and standardized procedures for sample processing are both essential to valid results.


1972 ◽  
Vol 57 (1) ◽  
pp. 89-94 ◽  
Author(s):  
G. M. Brittin ◽  
H. Rafinia ◽  
D. Raval ◽  
M. Werner ◽  
D. Brown

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