The effect of fibronectin on cytoskeleton structure and transepithelial resistance of alveolar type II cells in primary culture

1993 ◽  
Vol 64 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Kazuhiro Sugahara ◽  
Taketoshi Kiyota ◽  
Richard A. F. Clark ◽  
Robert J. Mason
Keyword(s):  
Primary Culture ◽  
Transepithelial Resistance ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells

Primary culture of rat alveolar type II cells on floating collagen membranes

1980 ◽  
Vol 128 (2) ◽  
pp. 363-374 ◽  
Author(s):  
Eugene F. Geppert ◽  
Mary C. Williams ◽  
Robert J. Mason
Keyword(s):  
Primary Culture ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Collagen Membranes

Secretory granule calcium loss after isolation of rat alveolar type II cells

1991 ◽  
Vol 260 (2) ◽  
pp. L129-L135 ◽  
Author(s):  
R. G. Eckenhoff ◽  
S. R. Rannels ◽  
A. B. Fisher
Keyword(s):  
Primary Culture ◽  
Sulfur Content ◽  
Lamellar Body ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Lamellar Bodies ◽  
Isolated Cells ◽  
Alveolar Type Ii Cells ◽  
Type Ii Cell

Morphological change and lamellar body loss suggests that alveolar type II cells rapidly de- or redifferentiate after several days of primary culture. To determine whether type II cells or lamellar body compositional changes precede these obvious morphological changes, we examined the in situ elemental composition of lamellar bodies and type II cells from intact lung and at different times after isolation using electron probe microanalysis (EPMA). Isolated cells were prepared by standard methods and plated on either tissue culture plastic or kept in suspension with stirrer flasks. Cell pellets obtained at 0, 3, 24, and 48 h after isolation were rapidly frozen, and thin freeze-dried cryosections were prepared and examined cold in a transmission electron microscope equipped for EPMA. Eight to ten type II cells from each of three to four different preparations for each time period were analyzed. A rapid, progressive, and sustained fall in lamellar body calcium and sulfur content occurred by 48 h of primary culture, suggesting rapid alteration in calcium and protein metabolism by type II cells and/or lamellar bodies after isolation. Also, marked changes in type II cell cytoplasmic Na and K occurred in freshly isolated cells, with incomplete normalization by 48 h. Culture on laminin-enriched Matrigel for 1 wk increased both lamellar body calcium or sulfur content, but 100 nM dexamethasone had no effect. Lamellar body calcium accumulation appears to be a very sensitive index of differentiated type II cell function.

scholarly journals Transepithelial transport by pulmonary alveolar type II cells in primary culture.

1982 ◽  
Vol 79 (19) ◽  
pp. 6033-6037 ◽  
Author(s):  
R. J. Mason ◽  
M. C. Williams ◽  
J. H. Widdicombe ◽  
M. J. Sanders ◽  
D. S. Misfeldt ◽  
...  
Keyword(s):  
Primary Culture ◽  
Transepithelial Transport ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells

Effects of interleukin-1beta on DNA synthesis in rat alveolar type II cells in primary culture

Respirology ◽  
1999 ◽  
Vol 4 (2) ◽  
pp. 139-145 ◽  
Author(s):  
Guan Hu Yang ◽  
Kazuhiro Osanai ◽  
Keiji Takahashi
Keyword(s):  
Dna Synthesis ◽  
Primary Culture ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Interleukin 1Beta

scholarly journals Hypoxia reduces endothelin production by rat alveolar type II cells in primary culture

1998 ◽  
Vol 11 (2) ◽  
pp. 392-399 ◽  
Author(s):  
B. Crestani ◽  
C. Odoux ◽  
C. Rolland ◽  
F. Moldovan ◽  
P. Cornillet ◽  
...  
Keyword(s):  
Primary Culture ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells
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