Effects ofddt and malathion on tissue succinic dehydrogenase activity (sdh) and lactic dehydrogenase isoenzymes (LDH) ofSarotherodon mossamhicus (Peters)

1985 ◽  
Vol 94 (5) ◽  
pp. 527-531 ◽  
Author(s):  
K Ramalingam
Keyword(s):  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Lactic Dehydrogenase ◽  
Succinic Dehydrogenase Activity

scholarly journals AN OSMIOPHILIC DISTYRYL DITETRAZOLIUM SALT (DS-NBT) FOR ULTRASTRUCTURAL DEHYDROGENASE ACTIVITY

1971 ◽  
Vol 19 (5) ◽  
pp. 273-285 ◽  
Author(s):  
ARNOLD M. SELIGMAN ◽  
IZHAK NIR ◽  
ROBERT E. PLAPINGER
Keyword(s):  
Skeletal Muscle ◽  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Lactic Dehydrogenase ◽  
Structural Features ◽  
Rat Skeletal Muscle ◽  
Succinic Dehydrogenase Activity ◽  
Tissue Penetration ◽  
Diaphorase Activity ◽  
Doubly Charged

The preparation of a new osmiophilic ditetrazolium salt (DS-NBT) containing two styryl groups, but retaining the essential structural features of nitro-BT, is given. DS-NBT may be used to demonstrate succinic dehydrogenase activity (SDH) faster than with nitro-BT and fully as rapidly as with TC-NBT. DS-NBT is more stable than TC-NBT and it is more reliably and more readily osmicated than TC-NBT. The ultrastructural sites of SDH and NADH2 diaphorase activity are similar to those shown for cytochrome oxidase activity with diaminobenzidine in rat heart. The sites of lactic dehydrogenase activity are found in the sarcoplasmic reticulum and mitochondria of rat skeletal muscle fixed in formaldehyde for 3 hr. Tissue penetration of the doubly charged ditetrazolium salt (DS-NBT) was facilitated by 40-sec sonication and by 5% dimethyl sulfoxide. Illustrative examples and controls are included.

Histochemical demonstration of succinic dehydrogenase activity during sequential molar development in the Swiss albino mouse

Histochemie ◽  
1972 ◽  
Vol 29 (4) ◽  
pp. 355-361
Author(s):  
D. Vincent Provenza ◽  
Yung-Feng Chang ◽  
Leslie P. Gartner ◽  
Richard M. Garlitz
Keyword(s):  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Histochemical Demonstration ◽  
Swiss Albino Mouse ◽  
Succinic Dehydrogenase Activity
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