Phytotoxins from Pyricularia grisea and Their Effect on Finger Millet

2006 ◽  
Vol 15 (1) ◽  
pp. 63-66 ◽  
Author(s):  
R. P. Sanmathi Kumar ◽  
L Shanthala ◽  
T. B. Anilkumar ◽  
Sudharshana L

2021 ◽  
Vol 15 (5) ◽  
pp. 217-230
Author(s):  
Manyasi Tracyline Jayo ◽  
K. Kimurto Paul ◽  
J. Mafurah Joseph


2013 ◽  
Vol 13 (2) ◽  
pp. 7-14
Author(s):  
Ram B Khadka ◽  
Sundar M Shrestha ◽  
Hira K Manandhar ◽  
Gopal BKC

Blast (Pyricularia grisea) is an economically important disease of rice and finger millet in Nepal. Isolates of the fungus from different hosts differed in their response in media for mycelial growth and sporulation. Radial mycelial growth (RMG) and days of sporulation (DOS) of P. grisea were studied by culturing three fungal isolates from rice, finger millet and Panicum sp. on six different media: prune agar (PA), oat meal agar (OMA), potato dextrose agar (PDA), finger millet leaf decoction agar (FLDA), finger millet polish agar (FPA) and finger millet meal agar (FMA). The highest RMG was found in the isolates from finger millet and the lowest in the isolates from rice. The shortest DOS (1 week) was found in the isolate from rice and the longest (>2 weeks) in the isolate from finger millet. Among the different media used, PA and OMA were found to be the best for mycelial growth and sporulation of the isolates both from rice and finger millet. The shape, color and compactness of the fungal colonies varied with the media and isolates used. Cross inoculation studies showed that the fungus isolates from rice were able to infect all the plant species (rice, finger millet, Panicum sp., Eleusine indica and Setaria sp.) while isolates from finger millet were only able to infect three plant species (E. coracana, Setaria sp. and E. indica). This shows that the weed management is more important in finger millet fields than in rice field to manage the blast disease; and growing of rice adjacent to finger millet field is dangerous for blast epidemics in finger millet since rice serves as the source of inoculums. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 7-14 DOI: http://dx.doi.org/10.3126/njst.v13i2.7707



Genetics ◽  
1999 ◽  
Vol 153 (2) ◽  
pp. 693-703
Author(s):  
Hitoshi Nakayashiki ◽  
Kanako Kiyotomi ◽  
Yukio Tosa ◽  
Shigeyuki Mayama

Abstract MAGGY is a gypsy-like LTR retrotransposon isolated from the blast fungus Pyricularia grisea (teleomorph, Magnaporthe grisea). We examined transposition of MAGGY in three P. grisea isolates (wheat, finger millet, and crabgrass pathogen), which did not originally possess a MAGGY element, and in two heterologous species of filamentous fungi, Colletotrichum lagenarium and P. zingiberi. Genomic Southern analysis of MAGGY transformants suggested that transposition of MAGGY occurred in all filamentous fungi tested. In contrast, no transposition was observed in any transformants with a modified MAGGY containing a 513-bp deletion in the reverse transcriptase domain. When a MAGGY derivative carrying an artificial intron was introduced into the wheat isolate of P. grisea and C. lagenarium, loss of the intron was observed. These results showed that MAGGY can undergo autonomous RNA-mediated transposition in heterologous filamentous fungi. The frequency of transposition differed among fungal species. MAGGY transposed actively in the wheat isolate of P. grisea and P. zingiberi, but transposition in C. lagenarium appeared to be rare. This is the first report that demonstrates active transposition of a fungal transposable element in heterologous hosts. Possible usage of MAGGY as a genetic tagging tool in filamentous fungi is discussed.



2014 ◽  
Vol 14 (2) ◽  
pp. 17-24
Author(s):  
RB Khadka ◽  
SM Shrestha ◽  
HK Manandhar ◽  
GB KC

To understand the pathogenic variability of Nepalese isolates of Pyricularia grisea pathogenic to finger millet and their differential interaction with this millet exotic lines and landraces, an experiment was conducted at Institute of Agriculture and Animal Sciences (IAAS), Rampur, Chitwan during August to October, 2008. Five Pyricularia isolates collected from Lumle, Pokhara, Gulmi, Kabre and Rampur were tested with five landraces Acc#4337, Acc#6202, Acc#456, KLB#184, Kabre and four exotic lines, PR#202, HR#911, SPFM#K2, and GPU#48 which have been used in national finger millet research programme by Hill Crop Research Programme (HCRP), Kabre, Dolakha. The experiment was laid out in 5x 9 factorial completely randomized designs with 4 replications in screen house. Eighteen-day-old seedlings grown in earthen pots were inoculated with a Pyricularia spore suspension (105ml-1) with the hand sprayer. Disease assessment was done in five days after inoculation. The aggressiveness of the isolates was noticed maximum in Pokhara and minimum in Gulmi isolates. Considerable pathogenic variations among the tested isolates were observed, however, no clear cut differential interactions were noticed in the interaction. The exotic lines showed comparatively resistant against the fungus reaction than the landraces. Among the tested lines Gpu#48, showed resistant reaction and Acc#4337 showed susceptible reaction in artificial inoculation. DOI: http://dx.doi.org/10.3126/njst.v14i2.10411 Nepal Journal of Science and Technology Vol. 14, No. 2 (2013) 17-24



2006 ◽  
Vol 5 (1) ◽  
pp. 47-50 ◽  
Author(s):  
R. Mahesh Kumar ◽  
K. Manjunatha ◽  
T.B. Anil Kumar ◽  
L. Sundharsha ◽  
B.S. Shiva Kumar ◽  
...  


2004 ◽  
Vol 20 (3) ◽  
pp. 251-256 ◽  
Author(s):  
R. Radjacommare ◽  
A. Ramanathan ◽  
A. Kandan ◽  
G.V. Sible ◽  
S. Harish ◽  
...  


2020 ◽  
Vol 28 (2) ◽  
pp. 155
Author(s):  
Devendra K. Gupta ◽  
A.K. Jain


2020 ◽  
Vol 35 (1) ◽  
pp. 36
Author(s):  
V.P. Prajapati ◽  
R.F. Chaudhary ◽  
A.J. Deshmukh ◽  
R.P. Bambharolia ◽  
N.K. Gajre


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