Cloning and Expression Analysis of bZIP Transcription Factor from Finger Millet (Eleusine coracana L.) Under Abiotic Stress Conditions

Basic leucine zipper (bZIP) transcription factors comprise one of the largest gene families in plants. They play a key role in almost every aspect of plant growth and development and also in biotic and abiotic stress tolerance. In this study, we were attempted to study characterization of bZIP, a transcription factor from a climate smart cereal finger millet (Eleusine coracana L.). Seeds of Eleusine coracana (finger millet) was purchase from local market and were grown under field conditions drought and salt stress conditions. In this study, EcbZIP gene was isolated from finger millet, cloned into DH5α cells, screened by using colony PCR and expression analysis in response to two abiotic stresses was carried out by using qRT PCR. EcbZIP coding DNA sequence and protein sequence were retrieved from NCBI Nucleotide Database and Genpept of Accession number KP033192.1 and AJP67539.1 and validated by using SMART (simple modular architecture tool) Domain Tool. Cloning and expression studies were carried out using standardized molecular biology protocol. Results depicted that EcbZIP transcription factor showed significant upregulation under both salt and drought stress conditions, indicating that it plays an important role in tolerance towards these stresses. In conclusion, expression analysis of bZIP gene from finger millet seed cultivar ML-365 showed 5-fold upregulation to salt stress to drought stress and 8-fold upregulation to salt stress. Hence, it can serve as a candidate gene for improving abiotic stress tolerance and can be helpful in enhancing the crop productivity under stress conditions.

Comparative Study of AMMI- and BLUP-Based Simultaneous Selection for Grain Yield and Stability of Finger Millet [Eleusine coracana (L.) Gaertn.] Genotypes

Finger millet, an orphan crop, possesses immense potential in mitigating climate change and could offer threefold security in terms of food, fodder, and nutrition. It is mostly cultivated as a subsistence crop in the marginal areas of plains and hills. Considering the changes in climate inclusive of recurrent weather vagaries witnessed every year, it is crucial to select stable, high-yielding, area-specific, finger millet cultivars. Sixty finger millet varieties released across the country were evaluated over six consecutive rainy seasons from 2011 to 2016 at the Agricultural Research Station, Vizianagaram. The genotype × environment interaction (GEI) was found to be significant in the combined ANOVA. Furthermore, the Additive Main effects and Multiplicative Interaction (AMMI) analysis asserted that genotypes and the GEI effects accounted for approximately 89% of the total variation. Strong positive associations were observed in an estimated set of eleven stability parameters which were chosen to identify stable genotypes. Furthermore, Non-parametric and Parametric Simultaneous Selection indices (NP-SSI and P-SSI) were calculated utilizing AMMI-based stability parameter (ASTAB), modified AMMI stability value (MASV), and Modified AMMI Stability Index (MASI) to identify stable high yielders. Both methods had inherent difficulties in ranking genotypes for SSI. To overcome this, the initial culling [i.e., SSI with culling strategy (C-SSI)] of genotypes was introduced for stability. In the C-SSI method, the top ten genotypes were above-average yielders, while those with below-average yield were observed in NP-SSI and P-SSI methods. Similarly, the estimation of best linear unbiased prediction (BLUP)-based simultaneous selections, such as harmonic mean of genotypic values (HMGV), relative performance of genotypic values (RPGV), and harmonic mean of relative performance of genotypic values (HMRPGV), revealed that none of the top ten entries had below-average yield. The study has proven that C-SSI and BLUP-based methods were equally worthy in the selection of high-yielding genotypes with stable performance. However, the C-SSI approach could be the best method to ensure that genotypes with a considerable amount of stability are selected. The multi-year trial SSI revealed that entries Indaf-9, Sri Chaitanya, PR-202, and A-404; and VL324 and VL146 were ascertained to be the most stable high-yielding genotypes among medium-to-late and early maturity groups, respectively.

Experimental Study on the Sound Absorption Properties of Finger Millet Straw, Darbha, and Ripe Bulrush Fibers

Nowadays, emerging noise pollution by external factors causes harmful diseases in human beings. The development of a bio-based filler or panel will help to eliminate some unwanted noise in working places and living rooms. This work aimed to develop an ecowaste fiber (leftover after harvesting)-based sound absorber and analyze its capabilities for sound absorption. The ecowaste fibers are collected by the gleaning process, i.e., the process of collecting leftovers from fields. The sound absorption capabilities of three natural fibers extracted from Eleusine coracana (Finger millet) straw, Desmostachya bipinnata (Darbha), and Typha domingensis (Ripe bulrush) plants are investigated in this study, both individually and in hybrid combinations. The sound absorption property mainly depends on factors such as porosity, flow resistivity, thickness, density, and tortuosity. Fiber length and fiber type play a significant role when fibers are arranged individually or in hybrid combinations. The stacking effect on the sound absorption coefficient of hybridized fiber arrangement was experimentally analyzed. The sound absorption coefficient (α) was found to be lower in the range of 1000 Hz–2500 Hz for all the combinations. As a homogenous fiber arrangement, the darbha fiber exhibited the better NRC (noise reduction coefficient) of 0.86 for 50 mm thickness among three different fibers and as a hybrid composition, ripe bulrush and darbha fibers exhibited NRC of 0.90 which is more capable of absorbing sound in the critical frequency range of 500 to 2000 Hz. These types of natural fiber fillers are highly capable of better sound absorbing and used in the applications such as classrooms, sound recording rooms, and theatres.