Effects of the administration of thyroid hormone on the plasma levels of atrial natriuretic peptides and on atrial myoendocrine cells in the rat: An immunochemical, ultrastructural, and stereological study

1992 ◽  
Vol 15 (10) ◽  
pp. 727-734 ◽  
Author(s):  
G. Cavallini ◽  
A. Clerico ◽  
M. Del Chicca ◽  
V. De Tata ◽  
Z. Gori ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Natriuretic Peptides ◽  
Plasma Levels ◽  
Atrial Natriuretic Peptides ◽  
Myoendocrine Cells ◽  
Atrial Natriuretic

scholarly journals An immunocytochemical study on co-localization of cathepsin B and atrial natriuretic peptides in secretory granules of atrial myoendocrine cells of rat heart.

1989 ◽  
Vol 37 (3) ◽  
pp. 347-351 ◽  
Author(s):  
T Watanabe ◽  
M Watanabe ◽  
Y Ishii ◽  
H Matsuba ◽  
S Kimura ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Cathepsin B ◽  
Rat Heart ◽  
Secretory Granules ◽  
Atrial Natriuretic Peptides ◽  
Thin Sections ◽  
Gold Particles ◽  
Atrial Tissue ◽  
Myoendocrine Cells ◽  
Atrial Natriuretic

To examine localization of cathepsin B, a representative lysosomal cysteine protease, in atrial myoendocrine cells of the rat heart, immunohistochemistry at the light and electron microscopic level was applied to the atrial tissue, using a monospecific antibody for rat liver cathepsin B. In serial semi-thin sections, immunoreactivity for cathepsin B and atrial natriuretic peptides (ANP) was detected in the para-nuclear region of atrial myoendocrine cells. Several large granules and many fine granules in the region of the cells were positively stained by the cathepsin B antibody. Gold particles indicating cathepsin B antigenicity labeled secretory granules in the cells, which were also labeled by those indicating ANP, using thin sections of the Lowicryl K4M-embedded material. Moreover, some granules labeled densely by immunogold particles for cathepsin B seemed to be lysosomes. By double immunostaining using thin sections of the Epon-embedded material, gold particles indicating cathepsin B and ANP antigenicities were co-localized in secretory granules of the cells. By enzyme assay, activity of cathepsin B was three times higher in atrial tissue than ventricular tissue. The results suggest that co-localization of cathepsin B and ANP in secretory granules is compatible with the possibility that cathepsin B participates in the maturation process of ANP.

Changes in the plasma levels of atrial natriuretic peptides during mineralocorticoid escape in man

1987 ◽  
Vol 72 (5) ◽  
pp. 531-539 ◽  
Author(s):  
Francesco P. Cappuccio ◽  
Nirmala D. Markandu ◽  
Martin G. Buckley ◽  
Giuseppe A. Sagnella ◽  
Angela C. Shore ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Healthy Volunteers ◽  
Natriuretic Peptide ◽  
Natriuretic Peptides ◽  
Plasma Levels ◽  
Sodium Intake ◽  
Atrial Natriuretic Peptides ◽  
Atrial Natriuretic

1. Plasma levels of atrial natriuretic peptide (ANP) were measured by radioimmunoassay in eight normal healthy volunteers before and during mineralocorticoid escape. 2. Mean plasma ANP on a fixed sodium intake before fludrocortisone was 6.5± sem 1.1 pg/ml. Within 24 h of fludrocortisone administration there was a significant increase in plasma ANP which continued to increase daily reaching a plateau by day 4 (14.9 ± 2.4 pg/ml) to day 7 (15.1 ± 2.6 pg/ml). 3. The rise in plasma ANP was closely related to the amount of sodium retained during the fludrocortisone treatment and the sodium ‘escape’ occurred by days 4 to 7. 4. These results support the concept that ANP could play an important hormonal role in overcoming the sodium-retaining effects of mineralocorticoids in man.

Raised plasma levels of atrial natriuretic peptides in Addison’s disease

1989 ◽  
Vol 12 (3) ◽  
pp. 205-207 ◽  
Author(s):  
F. P. Cappuccio ◽  
N. D. Markandu ◽  
M. G. Buckley ◽  
A. L. Sugden ◽  
G. A. Sagnella ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Plasma Levels ◽  
Addison’S Disease ◽  
Addison's Disease ◽  
Atrial Natriuretic Peptides ◽  
Atrial Natriuretic
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