1. Histamine in mast cells and basophils Nitric oxide modulates the inhibitory effect of cannabinoids on the immunological activation of guinea pig mast cells

The mechanism of vasoactive intestinal peptide (VIP)-induced pulmonary relaxation in tracheally perfused guinea pig lungs was defined with the use of inhibitors of nitric oxide synthase (NOS) and by direct measurement of nitric oxide (NO) equivalents recovered from lung perfusion fluid. Lungs treated with 200 microM NG-nitro-L-arginine were resistant to the relaxant effects of VIP in these lungs; the 50% inhibitory dose (ID50) for VIP was 32 nmol/kg (95% confidence interval, 16–79), which was approximately 100-fold greater than the ID50 of control lungs which was 0.39 nmol/kg, (0.16–0.79, P < 0.0001). This inhibitory effect could be overcome with excess L- but not D-arginine. In contrast, VIP-induced relaxation of isolated guinea pig trachea was not modified by inhibitors of NOS. To confirm that VIP infusion resulted in NO generation in whole lungs, we measured NO equivalents in lung effluent by two distinct technologies. We found that VIP injection caused a significant increase in NO equivalents from 0.11 +/- 0.04 microM to 0.78 +/- 0.15 microM (P < 0.05) and that this increase preceded VIP-induced pulmonary relaxation. Lungs pretreated with the putative guanylyl cyclase inhibitor methylene blue were less responsive to VIP [ID50 4.0 nmol/kg (1.5–10), P < 0.005 compared with control lungs], consistent with a physiologically significant guanosine 3',5'-cyclic monophosphate-dependent mechanism. Our data demonstrate that VIP has the capacity to relax whole lungs in part by stimulating the generation of NO.

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Sources and implications of basal nitric oxide in spontaneous contractions of guinea pig taenia caeci

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00273.2002 ◽

2003 ◽

Vol 285 (4) ◽

pp. G747-G753 ◽

Cited By ~ 5

Author(s):

Catalina Caballero-Alomar ◽

Carmen Santos ◽

Diego Lopez ◽

M. Teresa Mitjavila ◽

Pere Puig-Parellada

Keyword(s):

Nitric Oxide ◽

Guinea Pig ◽

Inhibitory Effect ◽

No Production ◽

Paramagnetic Resonance ◽

Synthase Inhibitor ◽

Spontaneous Contractions ◽

Electron Paramagnetic

We examined in vitro the source and role of basal nitric oxide (NO) in proximal segments of guinea pig taenia caeci in nonadrenergic, noncholinergic (NANC) conditions. Using electron paramagnetic resonance (EPR), we measured the effect of the NO synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME, 10–4 M), the neuronal blocker tetrodotoxin (TTX, 10–6 M), or both on spontaneous contractions and on the production of basal NO. Both l-NAME and TTX, when tested alone, increased the amplitude and frequency of contractions. NO production was abolished by l-NAME and was inhibited by 38% by TTX. When tested together, l-NAME in the presence of TTX or TTX in the presence of l-NAME had no further effect on the amplitude or frequency of spontaneous contractions, and the NO production was inhibited. These findings suggest that basal NO consists of TTX-sensitive and TTX-resistant components. The TTX-sensitive NO has an inhibitory effect on spontaneous contractions; the role of TTX-resistant NO is unknown.

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Inhibitory Effect of MY-1250 on Histamine Release from Rat Peritoneal Mast Cells and Guinea Pig Lung Fragments: The Elucidation of the Mechanism

Immunopharmacology and Immunotoxicology ◽

10.3109/08923979109019709 ◽

1991 ◽

Vol 13 (3) ◽

pp. 341-356 ◽

Cited By ~ 2

Author(s):

Chiaki Kamei ◽

Mitsunobu Mio ◽

Keiji Izushi ◽

Narihiko Yoshii ◽

Koshi Fujisawa ◽

...

Keyword(s):

Mast Cells ◽

Guinea Pig ◽

Histamine Release ◽

Inhibitory Effect ◽

Peritoneal Mast Cells ◽

Rat Peritoneal Mast Cells

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Imunotoxicology of carbon monoxide(CO): Hemin and CO abate the immunological activation of human basophils and of guinea-pig mast-cells

Toxicology Letters ◽

10.1016/s0378-4274(98)80714-5 ◽

1998 ◽

Vol 95 ◽

pp. 179

Author(s):

E. Masini ◽

M.G. Di Bello ◽

R. Baronti ◽

L.A. Berni ◽

B. Dannoui ◽

...

Keyword(s):

Carbon Monoxide ◽

Mast Cells ◽

Guinea Pig ◽

Immunological Activation ◽

Human Basophils

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Inhibitory effect of ginsenoside on the mediator release in the guinea pig lung mast cells activated by specific antigen-antibody reactions

International Journal of Immunopharmacology ◽

10.1016/s0192-0561(98)00062-9 ◽

1998 ◽

Vol 20 (11) ◽

pp. 625-641 ◽

Cited By ~ 32

Author(s):

J.Y. Ro ◽

Y.S. Ahn ◽

K.H. Kim

Keyword(s):

Mast Cells ◽

Guinea Pig ◽

Specific Antigen ◽

Inhibitory Effect ◽

Mediator Release ◽

Antigen Antibody

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Cholinergic and GABAergic regulation of nitric oxide synthesis in the guinea pig ileum

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1999.276.4.g862 ◽

1999 ◽

Vol 276 (4) ◽

pp. G862-G866 ◽

Cited By ~ 3

Author(s):

Katalina Hebeiss ◽

Heinz Kilbinger

Keyword(s):

Nitric Oxide ◽

Guinea Pig ◽

Myenteric Plexus ◽

Nicotinic Receptors ◽

Receptor Agonist ◽

Longitudinal Muscle ◽

Inhibitory Effect ◽

Nitric Oxide Synthesis ◽

Guinea Pig Ileum ◽

Threefold Increase

Nitric oxide (NO) synthesis was examined in intact longitudinal muscle-myenteric plexus preparations of the guinea pig ileum by determining the formation of [3H]citrulline during incubation with [3H]arginine. Spontaneous [3H]citrulline production after 30 min was 80–90 dpm/mg, which constituted ∼1% of the tissue radioactivity. Electrical stimulation (10 Hz) led to a threefold increase in [3H]citrulline formation. Removal of calcium from the medium or addition of N G-nitro-l-arginine strongly inhibited both spontaneous and electrically induced production of [3H]citrulline. TTX reduced the electrically induced but not spontaneous [3H]citrulline formation. The electrically induced formation of [3H]citrulline was diminished by (+)-tubocurarine and mecamylamine and enhanced by scopolamine, which suggests that endogenous ACh inhibits, via muscarinic receptors, and stimulates, via nicotinic receptors, the NO synthesis in the myenteric plexus. The GABAA receptor agonist muscimol and GABA also reduced the electrically evoked formation of [3H]citrulline, whereas baclofen was without effect. Bicuculline antagonized the inhibitory effect of GABA. It is concluded that nitrergic myenteric neurons are equipped with GABAAreceptors, which mediate inhibition of NO synthesis.

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The effect of a carbon monoxide-releasing molecule on the immunological activation of guinea-pig mast cells and human basophils

Inflammation Research ◽

10.1007/s00011-003-0303-8 ◽

2004 ◽

Vol 53 (0) ◽

pp. S9-S10 ◽

Cited By ~ 5

Author(s):

S. Pierpaolo ◽

P. F. Mannaioni ◽

A. Vannacci ◽

A. Di Felice ◽

G. Zagli ◽

...

Keyword(s):

Carbon Monoxide ◽

Mast Cells ◽

Guinea Pig ◽

Immunological Activation ◽

Human Basophils

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Anti-Anaphylactic Activity of Isoquercitrin (Quercetin-3-O-β-d-Glucose) in the Cardiovascular System of Animals

Biomedicines ◽

10.3390/biomedicines8060139 ◽

2020 ◽

Vol 8 (6) ◽

pp. 139

Author(s):

Jinbong Park

Keyword(s):

Mast Cells ◽

Guinea Pig ◽

Calcium Influx ◽

Inhibitory Effect ◽

Direct Inhibition ◽

Treatment And Prevention ◽

Marked Inhibitory Effect ◽

Pithed Rats ◽

Mesenteric Arterial Bed ◽

Potential Applications

Effects of isoquercitrin (IQ) on anaphylactic responses were examined in cardiovascular systems of experimental animals. In pithed rats, IQ at 30 and 100 mg/kg (intravenous) significantly blunted both the initial hypertensive and the ensuing hypotensive responses during anaphylaxis. Death rate and tachycardia were also significantly inhibited after the same IQ doses in these rats. In isolated guinea pig hearts, IQ infusion at 30–100 μg/mL markedly reduced anaphylaxis-related coronary flow decrease, contractile force change, and heart rate responses (both tachycardia and arrhythmia). Cardiac histamine and creatine kinase releases were similarly diminished by IQ during anaphylaxis in the isolated guinea pig hearts. In two different isolated guinea pig vasculatures, the pulmonary artery and mesenteric arterial bed, anaphylactic vasoconstriction was reduced by IQ 30 and 100 μg/mL. It was observed that IQ had a marked inhibitory effect on histamine release from rat mast cells, and this mechanism was suggested as the major anti-anaphylactic mechanism. Direct inhibition of histamine-induced muscle contraction did not seem to be relevant, but IQ treatment successfully repressed intracellular calcium influx/depletion in mast cells. Overall, this study provided evidence for the beneficial effect of IQ on cardiac anaphylaxis, thus suggesting its potential applications in the treatment and prevention of related diseases.

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