Effects of barley yellow mosaic disease resistant gene rym1 on the infection by strains of Barley yellow mosaic virus and Barley mild mosaic virus

2003 ◽  
Vol 106 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Y. Okada ◽  
S. Kashiwazaki ◽  
R. Kanatani ◽  
S. Arai ◽  
K. Ito
Keyword(s):  
Mosaic Virus ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Mild Mosaic ◽  
Yellow Mosaic Disease ◽  
Barley Yellow Mosaic Virus ◽  
Resistant Gene ◽  
Disease Resistant ◽  
Mild Mosaic Virus

Rapid visual detection method for barley yellow mosaic virus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Plant Disease ◽  
2020 ◽  
Author(s):  
Zhiwei Chen ◽  
Shuihua Mao ◽  
Wan Zhang ◽  
Xaorui Fan ◽  
Wenjing Wu ◽  
...  
Keyword(s):  
Food Security ◽  
Mosaic Virus ◽  
Reverse Transcription ◽  
Mosaic Disease ◽  
Isothermal Amplification ◽  
Yellow Mosaic Virus ◽  
Lamp Method ◽  
Yellow Mosaic Disease ◽  
Loop Mediated Isothermal Amplification ◽  
Barley Yellow Mosaic Virus

Barley yellow mosaic disease, caused mainly by barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV), is a devastating disease of barley and is a threat to Eurasian barley production. Early detection is essential for effective management of the pathogens and to assure food security. In the present study, a simple, rapid, specific, sensitive, and visual method was developed to detect BaYMV using loop-mediated isothermal amplification (LAMP). Two pairs of oligonucleotide primers (inner and outer primers) were designed to amplify the gene encoding the coat protein of BaYMV. The optimal conditions for the LAMP method were determined, and a one-step reverse transcription (RT)-LAMP method was also developed. Subsequently, the fastest processing time for RT-LAMP was determined. Among eight plant viruses examined using the LAMP method, only BaYMV was detectable, suggesting that the assay was highly specific. The RT-LAMP method was ten times more sensitive than the RT-PCR method in the sensitivity test. To further shorten the virus detection process, a dye was added to the RT-LAMP products, and positive reactions were simply read by the naked eye via a color change (from orange to light green) under visible light. Barley samples from the middle and lower reaches of the Yangtze River basin, where barley yellow mosaic disease broke out very seriously in 1970s, were detected by the newly established RT-LAMP method. The results showed that all samples were positive for BaYMV, indicating that the potential risk of the virus in these areas. This newly established LAMP/RT-LAMP method could be a promising tool for barley protection and food security control.

scholarly journals Studies on Cereal Soil-Borne Viruses in Poland

2010 ◽  
Vol 50 (4) ◽  
pp. 527-534 ◽  
Author(s):  
Małgorzata Jeżewska ◽  
Katarzyna Trzmiel
Keyword(s):  
Winter Wheat ◽  
Mosaic Virus ◽  
Special Interest ◽  
Yellow Mosaic Virus ◽  
Mild Mosaic ◽  
Barley Cultivars ◽  
Winter Triticale ◽  
Barley Yellow Mosaic Virus ◽  
Winter Cereals ◽  
Mild Mosaic Virus

Studies on Cereal Soil-Borne Viruses in PolandFour soil-borne cereal viruses have been identified in Poland, so far:Soil-borne cereal mosaic virus(SBCMV),Wheat spindle streak mosaic virus(WSSMV),Barley yellow mosaic virus(BaYMV) andBarley mild mosaic virus(BaMMV). SBCMV was identified in 1993 as a dangerous pathogen of winter cereals and became the object of special interest. Studies on the virus included its biological and molecular characterization, and investigations of the response of winter wheat and winter triticale cultivars on the SBCMV infection. Results of preliminary experiments aiming at the evaluation of the response of winter barley cultivars on barley yellow mosaic viruses were also presented.

​RCA-based Detection of Begomoviruses in Weed Ggenera Associated with Legumes in Southern Karnataka

2021 ◽  
Author(s):  
Sudeep Pandey ◽  
T.R. Girish ◽  
S. Basavaraj ◽  
A.S. Padmaja ◽  
N. Nagaraju
Keyword(s):  
Mosaic Virus ◽  
Rolling Circle Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Insect Vector ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Mungbean Yellow Mosaic Virus ◽  
Legume Crops

Background: Yellow mosaic disease (YMD) caused by begomoviruses transmitted through the insect vector Bemisia tabaci poses a serious threat to the production of legume crops. Methods: Season-long surveys were carried out for YMD occurrence in six different legume crops and associated natural weeds both symptomatic and asymptomatic across the districts of southern Karnataka, India. The samples were analyzed through RCA PCR using specific primer pairs. Result: Up to 94.1 per cent YMD incidence was recorded and nine weed species were commonly found associated with legume crops. The weeds viz., Ageratum conyzoides, Alternanthera sessilis, Commelina benghalensis and Euphorbia geniculata were abundantly found in the surveyed regions. The weeds were both symptomatic and asymptomatic. Rolling circle amplification coupled polymerase chain reaction method was employed to detect yellow mosaic virus in asymptomatic weeds. Phylogenetic analysis based on the sequences of PCR amplified products of weeds and symptomatic legumes revealed a close clustering of the weed samples with horsegram yellow mosaic virus, legume yellow mosaic virus and mungbean yellow mosaic virus. Overall, our data suggests the role of weed species associated with legume crops as alternative/collateral hosts of begomoviruses and their role in the epidemiology of yellow mosaic disease.

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