Identification of AFLP markers linked to the male fertility restorer gene of CMS (Moricandia arvensis) Brassica juncea and conversion to SCAR marker

2006 ◽  
Vol 114 (2) ◽  
pp. 385-392 ◽  
Author(s):  
Ashutosh ◽  
P. C. Sharma ◽  
Shyam Prakash ◽  
S. R. Bhat
Keyword(s):  
Brassica Juncea ◽  
Male Fertility ◽  
Scar Marker ◽  
Aflp Markers ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Moricandia Arvensis

Linkage Analysis of the Male‐Fertility Restorer Gene, Rf, in Cotton 1

Crop Science ◽  
1984 ◽  
Vol 24 (5) ◽  
pp. 992-994 ◽  
Author(s):  
R. J. Kohel ◽  
J. E. Quisenberry ◽  
R. E. Dilbeck
Keyword(s):  
Linkage Analysis ◽  
Male Fertility ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene

Development and mapping of AFLP markers linked to the sorghum fertility restorer gene rf4

2002 ◽  
Vol 104 (4) ◽  
pp. 577-585 ◽  
Author(s):  
L. Wen ◽  
H. V. Tang ◽  
W. Chen ◽  
R. Chang ◽  
D. R. Pring ◽  
...  
Keyword(s):  
Aflp Markers ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene

PPR-B-31: a new maintainer allele in the male-fertility restorer gene of radish (Raphanus sativus) Ogura cytoplasm

2015 ◽  
Vol 134 (5) ◽  
pp. 557-563
Author(s):  
Anna Hawliczek-Strulak ◽  
Grzegorz Bartoszewski ◽  
Renata Słomnicka ◽  
Aleksandra Korzeniewska ◽  
Régine Delourme ◽  
...  
Keyword(s):  
Male Fertility ◽  
Raphanus Sativus ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Ogura Cytoplasm ◽  
B 31

scholarly journals Development of fertility restorers of winter oilseed rape with low glucosinolate content for the CMS Ogu-INRA system

2009 ◽  
Vol 45 (No. 3) ◽  
pp. 123-127 ◽  
Author(s):  
R. Koprna ◽  
V. Kučera ◽  
I. Macháčková ◽  
J. Horáček ◽  
J. Ehrenbergerová
Keyword(s):  
Oilseed Rape ◽  
Scar Marker ◽  
Restorer Gene ◽  
Glucosinolate Content ◽  
Restorer Line ◽  
Winter Oilseed Rape ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Fertility Restorers ◽  
Selection Of

We have bred low glucosinolate (GSL) winter oilseed rape lines carrying the fertility restorer for the CMS Ogu-INRA system. The original restorer line BO20 contained 31μmol/g GSL in seeds, but by crossing this line with various low GSL CMS lines, followed by repeated selection of fertile segregants, we were able to obtain fertile lines with a mean GSL content in seeds of 11.8 μmol/g. This result confirmed that the gene(s) controlling the GSL content are not closely linked to the fertility restorer gene. The results confirm, that the SCAR marker SG34 is closely associated with the fertility restoring allele, and facilitates so the selection of fertile segregants; however, the marker is unable to distinguish between the homozygous RfRf and the heterozygous Rfrf genotypes.

scholarly journals Mapping of the New Fertility Restorer Gene Rf-PET2 Close to Rf1 on Linkage Group 13 in Sunflower (Helianthus annuus L.)

Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 269 ◽  
Author(s):  
Osama Sajer ◽  
Uta Schirmak ◽  
Sonia Hamrit ◽  
Renate Horn
Keyword(s):  
Linkage Group ◽  
Fertility Restoration ◽  
Aflp Markers ◽  
Restorer Gene ◽  
Hybrid Breeding ◽  
F1 Hybrids ◽  
Fertility Restorer ◽  
Group 13 ◽  
Fertility Restorer Gene ◽  
Sts Markers

The PET2-cytoplasm represents a well characterized new source of cytoplasmic male sterility (CMS) in sunflower. It is distinct from the PET1-cytoplasm, used worldwide for commercial hybrid breeding, although it was, as PET1, derived from an interspecific cross between Helianthus. petiolaris and H. annuus. Fertility restoration is essential for the use of CMS PET2 in sunflower hybrid breeding. Markers closely linked to the fertility restorer gene are needed to build up a pool of restorer lines. Fertility-restored F1-hybrids RHA 265(PET2) × IH-51 showed pollen viability of 98.2% ± 1.2, indicating a sporophytic mode of fertility restoration. Segregation analyses in the F2-population of the cross RHA 265(PET2) × IH-51 revealed that this cross segregated for one major restorer gene Rf-PET2. Bulked-segregant analyses investigating 256 amplified fragment length polymorphism (AFLP) primer combinations revealed a high degree of polymorphism in this cross. Using a subset of 24 AFLP markers, three sequence-tagged site (STS) markers and three microsatellite markers, Rf-PET2 could be mapped to the distal region of linkage group 13 between ORS1030 and ORS630. Three AFLP markers linked to Rf-PET2 were cloned and sequenced. Homology search against the sunflower genome sequence of HanXRQ v1r1 confirmed the physical location of Rf-PET2 close to the restorer gene Rf1 for CMS PET1. STS markers were mapped that can now be used for marker-assisted selection.

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