PPR-B-31: a new maintainer allele in the male-fertility restorer gene of radish (Raphanus sativus) Ogura cytoplasm

2015 ◽  
Vol 134 (5) ◽  
pp. 557-563
Author(s):  
Anna Hawliczek-Strulak ◽  
Grzegorz Bartoszewski ◽  
Renata Słomnicka ◽  
Aleksandra Korzeniewska ◽  
Régine Delourme ◽  
...  
Keyword(s):  
Male Fertility ◽  
Raphanus Sativus ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Ogura Cytoplasm ◽  
B 31

Linkage Analysis of the Male‐Fertility Restorer Gene, Rf, in Cotton 1

Crop Science ◽  
1984 ◽  
Vol 24 (5) ◽  
pp. 992-994 ◽  
Author(s):  
R. J. Kohel ◽  
J. E. Quisenberry ◽  
R. E. Dilbeck
Keyword(s):  
Linkage Analysis ◽  
Male Fertility ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene

scholarly journals A single nucleotide substitution in the coding region of Ogura male sterile gene, orf138, determines effectiveness of a fertility restorer gene, Rfo, in radish

2021 ◽  
Author(s):  
Hiroshi Yamagishi ◽  
Megumi Jikuya ◽  
Kanako Okushiro ◽  
Ayako Hashimoto ◽  
Asumi Fukunaga ◽  
...  
Keyword(s):  
Male Sterility ◽  
Nucleotide Substitution ◽  
Fertility Restoration ◽  
Type A ◽  
Restorer Gene ◽  
Single Nucleotide Substitution ◽  
Coding Region ◽  
Fertility Restorer ◽  
Single Nucleotide ◽  
Fertility Restorer Gene

AbstractCytoplasmic male sterility (CMS) observed in many plants leads defect in the production of functional pollen, while the expression of CMS is suppressed by a fertility restorer gene in the nuclear genome. Ogura CMS of radish is induced by a mitochondrial orf138, and a fertility restorer gene, Rfo, encodes a P-type PPR protein, ORF687, acting at the translational level. But, the exact function of ORF687 is still unclear. We found a Japanese variety showing male sterility even in the presence of Rfo. We examined the pollen fertility, Rfo expression, and orf138 mRNA in progenies of this variety. The progeny with Type H orf138 and Rfo showed male sterility when their orf138 mRNA was unprocessed within the coding region. By contrast, all progeny with Type A orf138 were fertile though orf138 mRNA remained unprocessed in the coding region, demonstrating that ORF687 functions on Type A but not on Type H. In silico analysis suggested a specific binding site of ORF687 in the coding region, not the 5′ untranslated region estimated previously, of Type A. A single nucleotide substitution in the putative binding site diminishes affinity of ORF687 in Type H and is most likely the cause of the ineffectiveness of ORF687. Furthermore, fertility restoration by RNA processing at a novel site in some progeny plants indicated a new and the third fertility restorer gene, Rfs, for orf138. This study clarified that direct ORF687 binding to the coding region of orf138 is essential for fertility restoration by Rfo.

Characterization of a radish introgression carrying the Ogura fertility restorer gene Rfo in rapeseed, using the Arabidopsis genome sequence and radish genetic mapping

2003 ◽  
Vol 107 (8) ◽  
pp. 1442-1451 ◽  
Author(s):  
Sandra Giancola ◽  
Sylvie Marhadour ◽  
Sophie Desloire ◽  
Vanessa Clouet ◽  
Hélène Falentin-Guyomarc'h ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Genome Sequence ◽  
Restorer Gene ◽  
Arabidopsis Genome ◽  
Fertility Restorer ◽  
Fertility Restorer Gene
Sign in / Sign up

Export Citation Format

Share Document