Molecular mapping of the rf1 gene for pollen fertility restoration in sorghum (Sorghum bicolor L.)

2001 ◽  
Vol 102 (8) ◽  
pp. 1206-1212 ◽  
Author(s):  
R. R. Klein ◽  
P. E. Klein ◽  
A. K. Chhabra ◽  
J. Dong ◽  
S. Pammi ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Pollen Fertility ◽  
Molecular Mapping ◽  
Fertility Restoration ◽  
Sorghum Bicolor L

Molecular mapping and candidate gene identification of the Rf2 gene for pollen fertility restoration in sorghum [Sorghum bicolor (L.) Moench]

2010 ◽  
Vol 120 (7) ◽  
pp. 1279-1287 ◽  
Author(s):  
D. R. Jordan ◽  
Emma S. Mace ◽  
R. G. Henzell ◽  
P. E. Klein ◽  
R. R. Klein
Keyword(s):  
Sorghum Bicolor ◽  
Candidate Gene ◽  
Pollen Fertility ◽  
Molecular Mapping ◽  
Fertility Restoration ◽  
Gene Identification ◽  
Candidate Gene Identification ◽  
Sorghum Bicolor L

Mapping and characterization of Rf 5 : a new gene conditioning pollen fertility restoration in A1 and A2 cytoplasm in sorghum (Sorghum bicolor (L.) Moench)

2011 ◽  
Vol 123 (3) ◽  
pp. 383-396 ◽  
Author(s):  
D. R. Jordan ◽  
R. R. Klein ◽  
K. G. Sakrewski ◽  
R. G. Henzell ◽  
P. E. Klein ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Pollen Fertility ◽  
Fertility Restoration ◽  
New Gene ◽  
Sorghum Bicolor L

scholarly journals Molecular mapping of genomic regions harbouring QTLs for root and yield traits in sorghum (Sorghum bicolor L. Moench)

2013 ◽  
Vol 19 (3) ◽  
pp. 409-419 ◽  
Author(s):  
Rajkumar ◽  
B. Fakrudin ◽  
S. P. Kavil ◽  
Y. Girma ◽  
S. S. Arun ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Molecular Mapping ◽  
Yield Traits ◽  
Genomic Regions ◽  
Sorghum Bicolor L

Molecular mapping and characterization of BLMC, a locus for profuse wax (bloom) and enhanced cuticular features of Sorghum (Sorghum bicolor (L.) Moench.)

2008 ◽  
Vol 118 (3) ◽  
pp. 423-431 ◽  
Author(s):  
Gloria B. Burow ◽  
Cleve D. Franks ◽  
Veronica Acosta-Martinez ◽  
Zhanguo Xin
Keyword(s):  
Sorghum Bicolor ◽  
Molecular Mapping ◽  
Sorghum Bicolor L

scholarly journals Molecular mapping of the brace root traits in sorghum (Sorghum bicolor L. Moench)

2014 ◽  
Vol 64 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Ronggai Li ◽  
Yucui Han ◽  
Peng Lv ◽  
Ruiheng Du ◽  
Guoqing Liu
Keyword(s):  
Sorghum Bicolor ◽  
Molecular Mapping ◽  
Root Traits ◽  
Sorghum Bicolor L

scholarly journals Identification of stable restorers for diverse cytoplasmic source in Sorghum [Sorghum bicolor (L.) Moench.]

2020 ◽  
Vol 80 (02) ◽  
Author(s):  
N. Sandeep ◽  
Basavaraj Biradar
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Sorghum Bicolor ◽  
Seed Set ◽  
Fertility Restoration ◽  
Male Sterile ◽  
Commercial Exploitation ◽  
M 31 ◽  
Fertility Restorers ◽  
Sorghum Bicolor L ◽  
Sorghum Germplasm

In sorghum [Sorghum bicolor (L.) Moench.], milo is the lone source of cytoplasmic male sterility (CMS) extensively used in production of commercial hybrids. The hazards of such a narrow cytoplasmic base are apparent and consequently new diverse sources like A2, A3, A4, maldandi, VZM and G1 were identified. However, the commercial exploitation of these sources has not been possible because of the difficulty in fertility restoration on these sources. The present study was, therefore, undertaken to test different sorghum germplasm lines for their fertility restoration/maintenance on maldandi and milo cytoplasm. Twenty-five diverse accession were selected from minicore collection and crossed with two male sterile lines viz., 104A (milo) and M 31-2A (maldandi). The resulting F1s were classified as fertility restorers and maintainers based on seed set on bagged ear heads. The restoration studies indicated that 2 lines on maldandi and 6 on milo cytoplasm showed strong fertility restoration (> 90 % seed set).

Inheritance and molecular mapping of Rf6 locus with pollen fertility restoration ability on A1 and A2 cytoplasms in sorghum

Plant Science ◽  
2015 ◽  
Vol 238 ◽  
pp. 73-80 ◽  
Author(s):  
Praveen M ◽  
Anurag Uttam G ◽  
Suneetha N ◽  
Umakanth Av ◽  
J.V. Patil ◽  
...  
Keyword(s):  
Pollen Fertility ◽  
Molecular Mapping ◽  
Fertility Restoration ◽  
Restoration Ability

scholarly journals Development and validation of CAPS-marker associated with the Rf2 gene in sorghum (Sorghum bicolor (L.) Moench)

2021 ◽  
Vol 4 (2) ◽  
pp. 38-47
Author(s):  
E. E. Radchenko ◽  
N. V. Alpatieva ◽  
Yu. I. Karabitsina ◽  
M. K. Ryazanova ◽  
E. B. Kuznetsova ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Pollen Fertility ◽  
Fertility Restoration ◽  
Hybrid Breeding ◽  
Sequence Variant ◽  
Chromosome 2 ◽  
Functional Alleles ◽  
Ppr Genes ◽  
Development And Validation ◽  
Fertility Restorers

Background. The development of heterotic hybrids based on cytoplasmic male sterility (CMS) is the leading strategy in breeding sorghum (Sorghum bicolor (L.) Moench). The trait of pollen fertility restoration in forms with CMS A1 (milo), predominantly used in sorghum breeding, is determined by at least two dominant complementary genes Rf1 and Rf2, and also gene Rf5. The development of accessible molecular markers of sorghum Rf genes is highly relevant for hybrid breeding, since they can significantly accelerate the process of creating female sterile forms (A lines), sterility maintainers (B lines) and pollen fertility restorers (R lines).Material and methods. The studied material included 36 sorghum accessions from the VIR collection, which differed by the ability to restore pollen fertility in forms with A1-type CMS. The nucleotide polymorphism of 935 bp fragments of the PPR genes Sobic.002G057050, Sobic.002G054100, and Sobic.002G054200 located at the chromosome 2 was studied.Results. The fragments obtained with the use of a pair of 2459403fw and 2459403 primers were 935 bp long and included parts of three genes: Sobic.002G057050, Sobic.002G054100, Sobic.002G054200. For identifying the sequence variant Sobic.002G057050-1090 associated with the Rf2 gene, Tru9 I restrictase was chosen, which allows obtaining a 572 bp fragment unique for all the studied R lines. Such a marker was found in 10 sorghum lines from West China and Kyrgyzstan, which are widely used in breeding as fertility restorers. The fragment was found neither in three lines with sterile cytoplasm and their fertile analogues, nor in 7 accessions of kafir sorghum, which lacked functional alleles of Rf genes.Conclusions. It has been demonstrated that the marker can be used for selection and checking purity of R and B/A lines. It is also applicable for verifying hybridity of F1 seeds and analyzing hybrid populations from crosses of R lines 924-4, 928-1, 929-3, 931-1, 933-1/6, 1237-3, 1243-2, 1251, 1150-1, F10BC2 with A lines Nizkorosloe 81s, А-83 and А-10598. It may be suggested that the ability to restore pollen fertility in R lines, which lack the marker CAPS- 572, is determined by another Rf gene. The studied 935 bp fragment of Sobic.002G057050 harbours 22 SNP, therefore the development of CAPS-markers for their identification and differentiation can be promising.

Molecular mapping and candidate gene analysis of a new epicuticular wax locus in sorghum (Sorghum bicolor L. Moench)

2017 ◽  
Vol 130 (10) ◽  
pp. 2109-2125 ◽  
Author(s):  
G. Anurag Uttam ◽  
M. Praveen ◽  
Y. Venkateswara Rao ◽  
Vilas A. Tonapi ◽  
R. Madhusudhana
Keyword(s):  
Sorghum Bicolor ◽  
Candidate Gene ◽  
Molecular Mapping ◽  
Epicuticular Wax ◽  
Gene Analysis ◽  
Candidate Gene Analysis ◽  
Sorghum Bicolor L

Pollen Fertility and Seed Setting: Their Role in Deciding the Yield of Sorghum (Sorghum bicolor (L) Moench) under Low Temperature Regimes

2016 ◽  
Vol 7 (6) ◽  
pp. 1377-1382
Author(s):  
Ganapati Mukri ◽  
◽  
B. D. Biradar ◽  
G. M. Sajjanar ◽  
Ramesh Kumar ◽  
...  
Keyword(s):  
Low Temperature ◽  
Sorghum Bicolor ◽  
Pollen Fertility ◽  
Seed Setting ◽  
Temperature Regimes ◽  
Sorghum Bicolor L
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