Cytogenetic analysis of Lycopersicon esculentum (+) Solanum etuberosum somatic hybrids and their androgenetic regenerants

2001 ◽  
Vol 103 (2-3) ◽  
pp. 231-239 ◽  
Author(s):  
T. Gavrilenko ◽  
R. Thieme ◽  
V.-M. Rokka
Keyword(s):  
Lycopersicon Esculentum ◽  
Cytogenetic Analysis ◽  
Somatic Hybrids ◽  
Solanum Etuberosum

Chromosome pairing and potential for intergeneric recombination in some hypotetraploid somatic hybrids of Lycopersicon esculentum (+) Solanum tuberosum

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1032-1041 ◽  
Author(s):  
J. H. de Jong ◽  
A. M. A. Wolters ◽  
J. M. Kok ◽  
H. Verhaar ◽  
J. van Eden
Keyword(s):  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Synaptonemal Complex ◽  
Chromosome Pairing ◽  
Somatic Hybrids ◽  
Cytogenetic Study ◽  
Unreduced Gametes ◽  
Root Tip ◽  
Prophase I ◽  
Metaphase I

Three somatic hybrids resulting from protoplast fusions of a diploid kanamycin-resistant line of tomato (Lycopersicon esculentum) and a dihaploid hygromycin-resistant transformant of a monohaploid potato (Solanum tuberosum) line were used for a cytogenetic study on chromosome pairing and meiotic recombination. Chromosome counts in root-tip meristem cells revealed two hypotetraploids with chromosome complements of 2n = 46 and one with 2n = 47. Electron microscope analyses of synaptonemal complex spreads of hypotonically burst protoplasts at mid prophase I showed abundant exchanges of pairing partners in multivalents involving as many as eight chromosomes. In the cells at late pachytene recombination nodules were found in multivalents on both sides of pairing partner exchanges, indicating recombination at both homologous and homoeologous sites. Light microscope observations of pollen mother cells at late diakinesis and metaphase I also revealed multivalents, though their occurrence in low frequencies betrays the reduction of multivalent number and complexity. Precocious separation of half bivalents at metaphase I and lagging of univalents at anaphase I were observed frequently. Bridges, which may result from an apparent inversion loop found in the synaptonemal complexes of a mid prophase I nucleus, were also quite common at anaphase I, though the expected accompanying fragments could be detected in only a few cells. Most striking were the high frequencies of first division restitution in preparations at metaphase II/anaphase II, giving rise to unreduced gametes. In spite of the expected high numbers of balanced haploid and diploid gametes, male fertility, as revealed by pollen staining, was found to be negligible.Key words: synaptonemal complex, recombination, chromosome pairing, somatic hybrid, Lycopersicon esculentum (+) Solanum tuberosum.

Asymmetric somatic hybrids between Lycopersicon esculentum and irradiated Lycopersicon peruvianum

1990 ◽  
Vol 80 (5) ◽  
pp. 665-672 ◽  
Author(s):  
J. Wijbrandi ◽  
A. M. A. Wolters ◽  
M. Koornneef
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrids ◽  
Lycopersicon Peruvianum ◽  
Asymmetric Somatic Hybrids

Cytological characterization of potato - Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 987-992 ◽  
Author(s):  
F Dong ◽  
R G Novy ◽  
J P Helgeson ◽  
J Jiang
Keyword(s):  
In Situ Hybridization ◽  
Dna Marker ◽  
Somatic Hybrids ◽  
Molecular Cytogenetics ◽  
Genomic In Situ Hybridization ◽  
Preferential Pairing ◽  
Potato Germplasm ◽  
Solanum Etuberosum

Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one from the potato parent 463-4 was lost in the hybrids. Three BC1 plants had exactly one set of S. etuberosum chromosomes. The other two BC1 plants either had one extra or one fewer S. etuberosum chromosome, possibly because their somatic hybrid parents had an extra or had lost one S. etuberosum chromosome. The presence of one set, or close to one set, of S. etuberosum chromosomes in all BC1 plants suggests a preferential pairing and segregation of the S. etuberosum chromosomes in the somatic hybrids. Two of the three BC2 plants had 52 chromosomes, deviating significantly from the expected chromosome number of 48. These results suggest poor pairing between S. etuberosum and S. tuberosum chromosomes in the BC1 plants. The present study demonstrates the importance of combining GISH and DNA marker analysis for a thorough characterization of potato germplasm containing chromosomes from different species.Key words: potato germplasm, Solanum etuberosum, molecular cytogenetics.

scholarly journals Fertile Somatic Hybrids between F1(Lycopersicon esculentum*L. peruvianum var. humifusum) and Solanum lycopersicoides.

1997 ◽  
Vol 47 (4) ◽  
pp. 327-333
Author(s):  
Asako Matsumoto ◽  
Shigeru Imanishi ◽  
Monzur Hossain ◽  
Alice Escalante ◽  
Hiroaki Egashira
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrids ◽  
Solanum Lycopersicoides

Analysis of plastome and chondriome genome types in potato somatic hybrids from Solanum tuberosum × Solanum etuberosum

Genome ◽  
2014 ◽  
Vol 57 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Jagesh K. Tiwari ◽  
Poonam Chandel ◽  
Bir Pal Singh ◽  
Vinay Bhardwaj
Keyword(s):  
Solanum Tuberosum ◽  
Somatic Hybrids ◽  
Gene Pools ◽  
Organelle Genomes ◽  
Cpdna Markers ◽  
Genomic Regions ◽  
Solanum Etuberosum ◽  
Unique Potential ◽  
Conserved Genomic Regions ◽  
Mtdna Markers

Cytoplasm types of the potato somatic hybrids from Solanum tuberosum × Solanum etuberosum were analysed using chloroplast (cp) and mitochondrial (mt) organelle genomes-specific markers. Of the 29 markers (15 cpDNA and 14 mtDNA) amplified in the 26 genotypes, 5 cpDNA (H3, NTCP4, NTCP8, NTCP9, and ALC1/ALC3) and 13 mtDNA markers showed polymorphism. The cluster analysis based on the mtDNA markers detected higher diversity compared with the cpDNA markers. Presence of new mtDNA fragments of the markers, namely, T11-2, Nsm1, pumD, Nsm3, and Nsm4, were observed, while monomorphic loci revealed highly conserved genomic regions in the somatic hybrids. The study revealed that the somatic hybrids had diverse cytoplasm types consisting predominantly of T-, W-, and C-, with a few A- and S-type cp genomes; and α-, β-, and γ-type mt genomes. Somatic hybridization has unique potential to widen the cytoplasm types of the cultivated gene pools from wild species through introgression by breeding methods.

CYTOGENETIC ANALYSIS OF CHROMOSOMAL TRANSLOCATIONS IN THE TOMATO: PREFERENTIAL BREAKAGE IN HETEROCHROMATIN

1980 ◽  
Vol 22 (3) ◽  
pp. 333-341 ◽  
Author(s):  
B. S. Gill ◽  
C. R. Burnham ◽  
G. R. Stringam ◽  
J. T. Stout ◽  
W. H. Weinheimer
Keyword(s):  
Lycopersicon Esculentum ◽  
Cytogenetic Analysis ◽  
Chromosome Length ◽  
Chromosomal Translocations ◽  
Pachytene Chromosome ◽  
Lycopersicon Esculentum Mill ◽  
Mode Of Production

A tester set of eight translocations has been selected involving all 12 chromosomes of tomato (Lycopersicon esculentum Mill.). Using the entire tester set, seven other translocations were identified. Five others were identified, using information from crosses with certain of the testers and with additional translocations. Three lines are homozygous for two translocations (2 04 in the heterozygotes). There are several instances in which more than one stock involves the same two chromosomes. Cytological analyses of tomato translocations show that breakages that give rise to translocation chromosomes occur more frequently in centromeric and heterochromatic regions than in euchromatic and are not distributed according to pachytene chromosome length. A list of available tomato translocations with information on mode of production, source, and breakpoints is included.

Chloroplast and mitochondrial DNA composition of triploid and tetraploid somatic hybrids between Lycopersicon esculentum and Solanum tuberosum

1995 ◽  
Vol 90 (2) ◽  
pp. 285-293 ◽  
Author(s):  
A. M. A. Wolters ◽  
H. C. H. Schoenmakers ◽  
M. Koornneef
Keyword(s):  
Mitochondrial Dna ◽  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Somatic Hybrids

Selection and characterization of somatic hybrids between Lycopersicon esculentum and Lycopersicon peruvianum

Plant Science ◽  
1990 ◽  
Vol 70 (2) ◽  
pp. 197-208 ◽  
Author(s):  
J. Wijbrandi ◽  
W. Van Capelle ◽  
C.J. Hanhart ◽  
E.P. Van Loenen Martinet-Schuringa ◽  
M. Koornneef
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrids ◽  
Lycopersicon Peruvianum

Non-random inheritance of organellar genomes in symmetric and asymmetric somatic hybrids between Lycopersicon esculentum and L. pennellii

1992 ◽  
Vol 84-84 (3-4) ◽  
pp. 435-442 ◽  
Author(s):  
A. B. Bonnema ◽  
J. M. Melzer ◽  
L. W. Murray ◽  
M. A. O'Connell
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrids ◽  
Asymmetric Somatic Hybrids ◽  
Organellar Genomes

Somatic hybrids between Solanum etuberosum and diploid, tuber bearing Solanum clones

1994 ◽  
Vol 89 (6) ◽  
pp. 775-782 ◽  
Author(s):  
R. G. Novy ◽  
J. P. Helgeson
Keyword(s):  
Somatic Hybrids ◽  
Solanum Etuberosum
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