Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphthalene derivatives for amperometric determination of cholesterol

2003 ◽  
Vol 377 (2) ◽  
pp. 273-280 ◽  
Author(s):  
J. C. Vidal ◽  
E. Garcia-Ruiz ◽  
J. Espuelas ◽  
T. Aramendia ◽  
J. R. Castillo
Keyword(s):  
Cholesterol Oxidase ◽  
Cholesterol Esterase ◽  
Amperometric Determination

scholarly journals A simple method for the determination of the cholesterol esterase activity.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Agnieszka Ewa Stępień ◽  
Mykhailo Gonchar
Keyword(s):  
Human Serum ◽  
Esterase Activity ◽  
Cholesterol Oxidase ◽  
Cholesterol Esterase ◽  
Cholesterol Esters ◽  
Simple Method ◽  
Bacterial Enzyme ◽  
Low Costs ◽  
Hydrolysis Of

The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.

Amperometric Determination of Total Cholesterol in Serum, with Use of Immobilized Cholesterol Ester Hydrolase and Cholesterol Oxidase

1977 ◽  
Vol 23 (4) ◽  
pp. 671-676 ◽  
Author(s):  
Hua-shan Huang ◽  
Shia S Kuan ◽  
Guilbault George G
Keyword(s):  
Serum Cholesterol ◽  
Immobilized Enzyme ◽  
Cholesterol Oxidase ◽  
Immobilized Enzymes ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Amperometric Determination ◽  
Standard Calomel Electrode ◽  
Pass Through

Abstract We describe an electrochemical method for simple, rapid, and economical assay of total serum cholesterol with use of immobilized cholesterol esterase (EC 3.1.1.13) and cholesterol oxidase (EC 1.1.3.6). A rotating porous cell was specially designed to hold the immobilized enzymes firmly and to allow the reaction mixture to pass through the en¬zyme layer easily, thus catalyzing the enzymatic trans¬formation quickly. Hydrogen peroxide resulting from the catalytic reactions was measured amperometrically at +0.60 V vs. a standard calomel electrode. The calibration curve for total serum cholesterol was linear from 0 to 5.00 g/liter. The method is specific, precise, and inexpensive. Our results correlate well with those obtained by the method of Abell et al. [Stand. Methods Clin. Chem. 2, 26 (1958)] , the correlation coefficient being 0.992. Ascorbic acid or bilirubin in concentrations up to 100 mg/liter do not interfere. The immobilized enzymes are stable, and the same immobilized-enzyme stirrer can be used for at least 200 accurate, reproducible assays.

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