Development of cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on oxygen electrode for the determination of total cholesterol in food samples

2007 ◽  
Vol 70 (2) ◽  
pp. 375-379 ◽  
Author(s):  
Anjan Kumar Basu ◽  
Parimal Chattopadhyay ◽  
Utpal Roychoudhuri ◽  
Runu Chakraborty
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Oxidase ◽  
Oxygen Electrode ◽  
Food Samples ◽  
Cholesterol Esterase ◽  
Cholesterol Biosensor

scholarly journals A simple method for the determination of the cholesterol esterase activity.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Agnieszka Ewa Stępień ◽  
Mykhailo Gonchar
Keyword(s):  
Human Serum ◽  
Esterase Activity ◽  
Cholesterol Oxidase ◽  
Cholesterol Esterase ◽  
Cholesterol Esters ◽  
Simple Method ◽  
Bacterial Enzyme ◽  
Low Costs ◽  
Hydrolysis Of

The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.

Direct Fluorometric Determination of Total Cholesterol in Serum Using Derivatized Cholesterol Oxidase

2000 ◽  
Vol 54 (8) ◽  
pp. 1157-1162 ◽  
Author(s):  
Javier Galbán ◽  
José F. Sierra ◽  
José M. López Sebastian ◽  
Susana de Marcos ◽  
Juan R. Castillo
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Cholesterol Oxidase ◽  
Enzymatic Reaction ◽  
Direct Determination ◽  
Analytical Signal ◽  
Cholesterol Concentration ◽  
Serum Samples ◽  
Response Range

In this paper the use of cholesterol oxidase derivatized with a fluorescein derivative is proposed for the direct determination of total cholesterol in blood serum. The method is based on the changes in the fluorescence of the solution during the enzymatic reaction (λexe = 498 nm and λem 519 nm). A mathematical model which relates the analytical signal to the total cholesterol concentration was developed, and the model can also be used to obtain some of the thermodynamic constants of the process. The method has a linear response range up to 70 mg/L of cholesterol, a detection limit of 2.5 mg/L, and the precision was 1.0% (40 mg/L cholesterol, n = 10). The method was applied to total cholesterol determination in blood serum samples. The results were compared to those obtained by a commercial analyzer, and statistically similar results were obtained. The use of derivatized cholesterol oxidase makes it possible to simplify the methodology normally used in this type of determination (the indicator reaction is avoided and the number of reagents reduced), with the added advantage that the analytical signal is independent of the concentrations of O2 and cholesterol oxidase.

An enzymatic assay for determining free and total cholesterol in tissue.

1978 ◽  
Vol 24 (3) ◽  
pp. 433-435 ◽  
Author(s):  
J L De Hoff ◽  
L M Davidson ◽  
D Kritchevsky
Keyword(s):  
Total Cholesterol ◽  
Rat Liver ◽  
Ferric Chloride ◽  
Cholesterol Oxidase ◽  
Enzymatic Assay ◽  
Cholesterol Esterase ◽  
Enzymatic Method ◽  
Liver Lipids

Abstract We describe a method for determining free and total cholesterol in extracts of rat-liver lipids by use of a cholesterol esterase/cholesterol oxidase enzymatic kit (Boehringer Mannheim Corp). The lipids are solubilized (as micelles) directly into the aqueous reagent mixture. Results of the enzymatic method correlate well with those obtained by an acid/ferric chloride assay of digitonin precipitates of the extracts. The enzymatic assay of free and total cholesterol in tissue offers advantages of simplicity over previously used methods.

scholarly journals Mediator-free biosensor using chitosan capped CdS quantum dots for detection of total cholesterol

RSC Advances ◽  
2015 ◽  
Vol 5 (57) ◽  
pp. 45928-45934 ◽  
Author(s):  
Hemant Dhyani ◽  
Md. Azahar Ali ◽  
Satyendra P. Pal ◽  
Saurabh Srivastava ◽  
Pratima R. Solanki ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Total Cholesterol ◽  
Cadmium Sulfide ◽  
Surface Functionalization ◽  
Cholesterol Oxidase ◽  
Cholesterol Esterase ◽  
Cds Quantum Dots ◽  
Oxidase Enzyme ◽  
Enzyme Molecules

An electrochemical mediator-free biosensor platform have been fabricated using in situ synthesized cadmium sulfide quantum dots embedded in chitosan via surface functionalization of cholesterol esterase and cholesterol oxidase enzyme molecules.

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