Fluorescence polarization immunoassay for rapid determination of dehydroepiandrosterone in human urine

2021 ◽  
Author(s):  
Huiyi Yang ◽  
Qiyi He ◽  
Sergei A. Eremin ◽  
Junkang Pan ◽  
Yikui Zou ◽  
...  
Keyword(s):  
Human Urine ◽  
Fluorescence Polarization ◽  
Rapid Determination ◽  
Fluorescence Polarization Immunoassay

Development and optimization of a fluorescence polarization immunoassay for orbifloxacin in milk

2014 ◽  
Vol 6 (11) ◽  
pp. 3849-3857 ◽  
Author(s):  
Tiejun Mi ◽  
Xiao Liang ◽  
Long Ding ◽  
Suxia Zhang ◽  
Sergei A. Eremin ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Rapid Determination ◽  
Fluorescence Polarization Immunoassay ◽  
Fluorescent Tracer

Rapid determination of orbifloxacin residue in milk by an optimized fluorescence polarization immunoassay based on a heterogeneous fluorescent tracer.

Cross-Reactivity of Anti-Digoxin Antibodies with Digitoxin Depends on Tracer Structure

1992 ◽  
Vol 38 (11) ◽  
pp. 2228-2232 ◽  
Author(s):  
R Stenzel ◽  
B Reckmann
Keyword(s):  
Hydroxy Group ◽  
Fluorescence Polarization ◽  
Cross Reactivity ◽  
Fluorescence Polarization Immunoassay ◽  
Exact Determination

Abstract The most common methods for measuring digoxin concentrations in serum are immunoassays. The prerequisite for exact determination of the digoxin value is an antibody that specifically binds digoxin. Because digitoxin differs from digoxin only in the C-12 hydroxy group, it is difficult to obtain anti-digoxin antibodies that do not cross-react with this compound. During the development of a fluorescence polarization immunoassay (FPIA) for digoxin, we investigated digoxin tracers with different structures. We found that in FPIA the digitoxin cross-reactivity of an antibody could be reduced by varying the structure of the tracer molecule.

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