Fluorescence Polarization Immunoassay for the Determination of Therapeutic Drug Levels in Human Plasma

1981 ◽  
Vol 5 (5) ◽  
pp. 236-240 ◽  
Author(s):  
M.E. Jolley
Keyword(s):  
Human Plasma ◽  
Fluorescence Polarization ◽  
Therapeutic Drug ◽  
Fluorescence Polarization Immunoassay ◽  
Drug Levels

Development and optimization of a fluorescence polarization immunoassay for orbifloxacin in milk

2014 ◽  
Vol 6 (11) ◽  
pp. 3849-3857 ◽  
Author(s):  
Tiejun Mi ◽  
Xiao Liang ◽  
Long Ding ◽  
Suxia Zhang ◽  
Sergei A. Eremin ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Rapid Determination ◽  
Fluorescence Polarization Immunoassay ◽  
Fluorescent Tracer

Rapid determination of orbifloxacin residue in milk by an optimized fluorescence polarization immunoassay based on a heterogeneous fluorescent tracer.

Monitoring tricyclic antidepressant concentrations in serum by fluorescence polarization immunoassay compared with gas chromatography and HPLC

1994 ◽  
Vol 40 (6) ◽  
pp. 929-933 ◽  
Author(s):  
M L Rao ◽  
U Staberock ◽  
P Baumann ◽  
C Hiemke ◽  
A Deister ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Fluorescence Polarization ◽  
Tricyclic Antidepressants ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Tricyclic Antidepressant ◽  
Therapeutic Drug ◽  
Fluorescence Polarization Immunoassay

Abstract The fluorescence polarization immunoassay (FPIA) developed by Abbott to diagnose intoxication with tricyclic antidepressants was adapted for therapeutic drug monitoring and validated with chromatograpic methods to investigate its potential for this use. We compared serum concentrations of tricyclic antidepressants in vivo and in vitro obtained by FPIA with those by gas chromatography and HPLC. For amitriptyline, imipramine, clomipramine, and doxepin, the detection limit of the FPIA was 72, 71, 64, and 72 nmol/L (approximately 20 micrograms/L), respectively; that by gas chromatography was 18, 18, and 16 nmol/L (approximately 5 micrograms/L) for amitriptyline, imipramine and clomipramine, respectively; with HPLC the lower limit of detection for doxepin was 36 nmol/L (10 micrograms/L). The intra- and interassay CVs ranged from 3% to 6%. In patients being treated with amitriptyline, imipramine, clomipramine, and doxepin, at steady-state the correlation coefficients between FPIA and GC/HPLC results for split samples were 0.95, 0.92, 0.90 and 0.70, respectively. However, the slopes were close to unity only for amitriptyline and doxepin, being 0.6 for imipramine and 1.9 for clomipramine.

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