Modulation of the Calmodulin-induced Inhibition of Sarcoplasmic Reticulum Calcium Release Channel (Ryanodine Receptor) by Sulfhydryl Oxidation in Single Channel Current Recordings and [ 3 H]Ryanodine Binding

2000 ◽  
Vol 174 (2) ◽  
pp. 105-120 ◽  
Author(s):  
J. Suko ◽  
G. Hellmann ◽  
H. Drobny
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Ryanodine Receptor ◽  
Calcium Release ◽  
Single Channel ◽  
Calcium Release Channel ◽  
Release Channel ◽  
Channel Current ◽  
Single Channel Current ◽  
Sulfhydryl Oxidation ◽  
Sarcoplasmic Reticulum Calcium

scholarly journals Effect of gadolinium on the ryanodine receptor/sarcoplasmic reticulum calcium release channel of skeletal muscle

FEBS Journal ◽  
2004 ◽  
Vol 272 (2) ◽  
pp. 464-471 ◽  
Author(s):  
Sándor Sárközi ◽  
Csaba Szegedi ◽  
Balázs Lukács ◽  
Michel Ronjat ◽  
István Jóna
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Ryanodine Receptor ◽  
Calcium Release ◽  
Calcium Release Channel ◽  
Release Channel ◽  
Sarcoplasmic Reticulum Calcium

Functional expression of cDNA encoding the calcium release channel (ryanodine receptor) of rabbit skeletal muscle sarcoplasmic reticulum in COS-1 cells

Biochemistry ◽  
1993 ◽  
Vol 32 (14) ◽  
pp. 3743-3753 ◽  
Author(s):  
S. R. Wayne Chen ◽  
Donna M. Vaughan ◽  
Judith A. Airey ◽  
Roberto Coronado ◽  
David H. MacLennan
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Ryanodine Receptor ◽  
Calcium Release ◽  
Functional Expression ◽  
Rabbit Skeletal Muscle ◽  
Calcium Release Channel ◽  
Release Channel

scholarly journals Sphingosylphosphocholine modulates the ryanodine receptor/calcium-release channel of cardiac sarcoplasmic reticulum membranes

1997 ◽  
Vol 322 (1) ◽  
pp. 327-333 ◽  
Author(s):  
Romeo BETTO ◽  
Alessandra TERESI ◽  
Federica TURCATO ◽  
Giovanni SALVIATI ◽  
Roger A. SABBADINI ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Ryanodine Receptor ◽  
Calcium Release ◽  
Cardiac Tissue ◽  
Ruthenium Red ◽  
Release Mechanism ◽  
Calcium Release Channel ◽  
Release Channel ◽  
Cardiac Sarcoplasmic Reticulum ◽  
High Calcium

Sphingosylphosphocholine (SPC) modulates Ca2+ release from isolated cardiac sarcoplasmic reticulum membranes; 50 ƁM SPC induces the release of 70Ő80% of the accumulated calcium. SPC releases calcium from cardiac sarcoplasmic reticulum through the ryanodine receptor, since the release is inhibited by the ryanodine receptor channel antagonists ryanodine, Ruthenium Red and sphingosine. In intact cardiac myocytes, even in the absence of extracellular calcium, SPC causes a rise in diastolic Ca2+, which is greatly reduced when the sarcoplasmic reticulum is depleted of Ca2+ by prior thapsigargin treatment. SPC action on the ryanodine receptor is Ca2+-dependent. SPC shifts to the left the Ca2+-dependence of [3H]ryanodine binding, but only at high pCa values, suggesting that SPC might increase the sensitivity to calcium of the Ca2+-induced Ca2+-release mechanism. At high calcium concentrations (pCa 4.0 or lower), where [3H]ryanodine binding is maximally stimulated, no effect of SPC is observed. We conclude that SPC releases calcium from cardiac sarcoplasmic reticulum membranes by activating the ryanodine receptor and possibly another intracellular Ca2+-release channel, the sphingolipid Ca2+-release-mediating protein of endoplasmic reticulum (SCaMPER) [Mao, Kim, Almenoff, Rudner, Kearney and Kindman (1996) Proc. Natl. Acad. Sci. U.S.A 93, 1993Ő1996], which we have identified for the first time in cardiac tissue.

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