scholarly journals Maturational Changes in Rabbit Renal Brush Border Membrane Vesicle Osmotic Water Permeability

1998 ◽  
Vol 164 (2) ◽  
pp. 177-185 ◽  
Author(s):  
R. Quigley ◽  
E.W. Harkins ◽  
P.J. Thomas
Keyword(s):  
Water Permeability ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicle ◽  
Brush Border Membrane Vesicle ◽  
Osmotic Water Permeability ◽  
Renal Brush Border Membrane ◽  
Osmotic Water ◽  
Renal Brush Border

scholarly journals Glucocorticoids increase osmotic water permeability (Pf) of neonatal rabbit renal brush border membrane vesicles

2005 ◽  
Vol 288 (5) ◽  
pp. R1417-R1421 ◽  
Author(s):  
Jaap Mulder ◽  
Sumana Chakravarty ◽  
Maha N. Haddad ◽  
Michel Baum ◽  
Raymond Quigley
Keyword(s):  
Membrane Fluidity ◽  
Water Permeability ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Protein Abundance ◽  
Osmotic Water Permeability ◽  
Dexamethasone Treatment ◽  
Renal Brush Border Membrane ◽  
Osmotic Water ◽  
Renal Brush Border

During postnatal maturation, there is an increase in renal brush border membrane vesicle (BBMV) osmotic water permeability and a parallel increase in aquaporin-1 (AQP1) protein abundance. The mechanisms responsible for these changes remain unknown. Because serum glucocorticoid levels rise postnatally and have previously been linked to other maturational changes in renal function, we examined the effects of glucocorticoids on osmotic (Pf) and diffusional (PDW) water permeability and AQP1 protein abundance of renal BBMV. Neonatal rabbits were treated with dexamethasone (10 μg/100 g) for three days and compared with control neonates and adults. Pfand PDWwere measured at 20°C with a stopped-flow apparatus using light-scattering and aminonaphthalene trisulfonic acid (ANTS) fluorescence, respectively. Pfwas significantly higher in BBMV from dexamethasone-treated neonates compared with vehicle-treated neonates, but remained lower than in BBMV from adults ( P < 0.05). PDWin dexamethasone and vehicle-treated neonatal BBMV was lower than in adult BBMV. Pf/PDWratio increased from neonate (5.1 ± 0.3) to dexamethasone (7.0 ± 0.1) and adult BBMV (6.3 ± 0.1). AQP1 expression was increased by dexamethasone treatment to adult levels. Membrane fluidity, which is inversely related to generalized polarization (GP) of steady-state laurdan fluorescence, was significantly higher in neonatal BBMV than both dexamethasone and adult BBMV (GP: neonate 0.285 ± 0.002, dexamethasone treatment 0.302 ± 0.006, and adult 0.300 ± 0.005; P < 0.05). These combined results show that dexamethasone-treatment during days 4–7 of life increases BBMV water permeability despite a decrease in membrane fluidity. This occurs by increasing channel-mediated water transport, as reflected in an increase in AQP1 protein abundance and a higher Pf/PDWratio. This mimics the maturational changes and suggests a physiological role for glucocorticoids in maturation of proximal tubule water transport.

scholarly journals Hypothyroidism Increases Osmotic Water Permeability (Pf) in the Developing Renal Brush Border Membrane

2003 ◽  
Vol 53 (6) ◽  
pp. 1001-1007 ◽  
Author(s):  
Jaap Mulder ◽  
Maha N Haddad ◽  
Kimberly Vernon ◽  
Michel Baum ◽  
Raymond Quigley
Keyword(s):  
Water Permeability ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Osmotic Water Permeability ◽  
Renal Brush Border Membrane ◽  
Osmotic Water ◽  
Renal Brush Border

scholarly journals Neonatal and Adult Rabbit Renal Brush Border Membrane Vesicle Solute Reflection Coefficients

Neonatology ◽  
1999 ◽  
Vol 76 (2) ◽  
pp. 106-113 ◽  
Author(s):  
Raymond Quigley ◽  
Marisa Flynn ◽  
Michel Baum
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicle ◽  
Adult Rabbit ◽  
Reflection Coefficients ◽  
Brush Border Membrane Vesicle ◽  
Renal Brush Border Membrane ◽  
Renal Brush Border

scholarly journals Maturational Changes in Rabbit Renal Brush Border Membrane Vesicle Urea Permeability

1999 ◽  
Vol 45 (1) ◽  
pp. 143-147 ◽  
Author(s):  
Raymond Quigley ◽  
Marisa Flynn ◽  
Michel Baum
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicle ◽  
Brush Border Membrane Vesicle ◽  
Urea Permeability ◽  
Renal Brush Border Membrane ◽  
Renal Brush Border
Sign in / Sign up

Export Citation Format

Share Document