Osmotic water permeability and solute reflection coefficients of rat kidney brush-border membrane vesicles

1986 ◽  
Vol 861 ◽  
pp. 395-397 ◽  
Author(s):  
J. Pratz ◽  
P. Ripoche ◽  
B. Corman
Keyword(s):  
Water Permeability ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Reflection Coefficients ◽  
Osmotic Water Permeability ◽  
Osmotic Water

Osmotic water permeability of rat intestinal brush border membrane vesicles: involvement of aquaporin-7 and aquaporin-8 and effect of metal ions

2007 ◽  
Vol 85 (6) ◽  
pp. 675-684 ◽  
Author(s):  
Simona Tritto ◽  
Giulia Gastaldi ◽  
Sergey Zelenin ◽  
Monica Grazioli ◽  
Maria Novella Orsenigo ◽  
...  
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Water Permeability ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Water Movement ◽  
Membrane Vesicles ◽  
Osmotic Water Permeability ◽  
Transfected Cells ◽  
Osmotic Water

Water channels AQP7 and AQP8 may be involved in transcellular water movement in the small intestine. We show that both AQP7 and AQP8 mRNA are expressed in rat small intestine. Immunoblot and immunohistochemistry experiments demonstrate that AQP7 and AQP8 proteins are present in the apical brush border membrane of intestinal epithelial cells. We investigated the effect of several metals and pH on the osmotic water permeability (Pf) of brush border membrane vesicles (BBMVs) and of AQP7 and AQP8 expressed in a cell line. Hg2+, Cu2+, and Zn2+ caused a significant decrease in the BBMV Pf, whereas Ni2+ and Li+ had no effect. AQP8-transfected cells showed a reduction in Pf in the presence of Hg2+ and Cu2+, whereas AQP7-transfected cells were insensitive to all tested metals. The Pf of both BBMVs and cells transfected with AQP7 and AQP8 was not affected by pH changes within the physiological range, and the Pf of BBMVs alone was not affected by phlorizin or amiloride. Our results indicate that AQP7 and AQP8 may play a role in water movement via the apical domain of small intestine epithelial cells. AQP8 may contribute to the water-imbalance-related clinical symptoms apparent after ingestion of high doses of Hg2+ and Cu2+.

scholarly journals Studies on the orientation of brush-border membrane vesicles

1978 ◽  
Vol 172 (1) ◽  
pp. 57-62 ◽  
Author(s):  
W Haase ◽  
A Schäfer ◽  
H Murer ◽  
R Kinne
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Freeze Fracture ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Immunological Methods ◽  
Kidney Cortex ◽  
Asymmetric Distribution ◽  
Rat Kidney Cortex

Orientation of rat renal and intestinal brush-border membrane vesicles was studied with two independent methods: electron-microscopic freeze-fracture technique and immunological methods. With the freeze-fracture technique a distinct asymmetric distribution of particles on the two membrane fracture faces was demonstrated; this was used as a criterion for orientation of the isolated membrane vesicles. For the immunological approach the accessibility or inaccessibility of aminopeptidase M localized on the outer surface of the cell membrane to antibodies was used. With both methods we showed that the brush-border membrane vesicles isolated from rat kidney cortex and from rat small intestine for transport studies are predominantly orientated right-side out.

Adenosine (Ado) uptake in brush-border membrane vesicles from rat kidney (BBM)

1991 ◽  
Vol 19 (3) ◽  
pp. 323S-323S ◽  
Author(s):  
J. SAYOS ◽  
J. J. CENTELLES ◽  
J. MALLOL ◽  
E. I. CANELA ◽  
C. LLUIS ◽  
...  
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles

Vitamin B6Uptake by Rat Kidney Cells and Brush-Border Membrane Vesicles

1990 ◽  
Vol 585 (1 Vitamin B6) ◽  
pp. 106-109 ◽  
Author(s):  
BARBARA B. BOWMAN ◽  
DONALD B. McCORMICK ◽  
ELIZABETH R. SMITH
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Kidney Cells

scholarly journals Sulphate-ion/sodium-ion co-transport by brush-border membrane vesicles isolated from rat kidney cortex

1979 ◽  
Vol 182 (1) ◽  
pp. 223-229 ◽  
Author(s):  
Heinrich Lücke ◽  
Gertraud Stange ◽  
Heini Murer
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Electrical Potential ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Kidney Cortex ◽  
Electrical Potential Difference ◽  
Univalent Cations ◽  
Rat Kidney Cortex

Uptake of SO42− into brush-border membrane vesicles isolated from rat kindey cortex by a Ca2+-precipitation method was investigated by using a rapid-filtration technique. Uptake of SO42− by the vesicles was osmotically sensitive and represented transport into an intra-vesicular space. Transport of SO42− by brush-border membranes was stimulated in the presence of Na+, compared with the presence of K+ or other univalent cations. A typical ‘overshoot’ phenomenon was observed in the presence of an NaCl gradient (100mm-Na+ outside/zero mm-Na+ inside). Radioactive-SO42− exchange was faster in the presence of Na+ than in the presence of K+. Addition of gramicidin-D, an ionophore for univalent cations, decreased the Na+-gradient-driven SO42− uptake. SO42− uptake was only saturable in the presence of Na+. Counter-transport of Na+-dependent SO42− transport was shown with MoO42− and S2O32−, but not with PO42−. Changing the electrical potential difference across the vesicle membrane by establishing different diffusion potentials (anion replacement; K+ gradient±valinomycin) was not able to alter Na+-dependent SO42− uptake. The experiments indicate the presence of an electroneutral Na+/SO42−-co-transport system in brush-border membrane vesicles isolated from rat kidney cortex.

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