Enzymatic conversion of d-galactose to d-tagatose: heterologous expression and characterisation of a thermostable l-arabinose isomerase from Thermoanaerobacter mathranii

2004 ◽  
Vol 64 (6) ◽  
pp. 816-822 ◽  
Author(s):  
F. J�rgensen ◽  
O. C. Hansen ◽  
P. Stougaard
Keyword(s):  
Heterologous Expression ◽  
Enzymatic Conversion ◽  
Arabinose Isomerase

Alternative Heterologous Expression of l-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 By Residual Whey Lactose Induction

2021 ◽  
Author(s):  
Ticiane C. de Souza ◽  
Ravena Casemiro Oliveira ◽  
Saulo Gonçalves Santiago Bezerra ◽  
Ricardo M. Manzo ◽  
Enrique J. Mammarella ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Enterococcus Faecium ◽  
Lactose Induction ◽  
Arabinose Isomerase

First Fluorescent Acetylspermidine Deacetylation Assay for HDAC10 Identifies Inhibitors of Neuroblastoma Cell Colony Growth That Increase Lysosome Accumulation

2020 ◽  
Author(s):  
Daniel Herp ◽  
Johannes Ridinger ◽  
Dina Robaa ◽  
Stephen A. Shinsky ◽  
Karin Schmidtkunz ◽  
...  
Keyword(s):  
Histone Deacetylases ◽  
High Throughput Screening ◽  
Neuroblastoma Cell ◽  
Hdac Inhibitors ◽  
Anticancer Therapy ◽  
Colony Growth ◽  
Autophagic Flux ◽  
Enzymatic Conversion ◽  
Lysine Deacetylase

Histone deacetylases (HDACs) are important epigenetic regulators involved in many diseases, esp. cancer. First HDAC inhibitors have been approved for anticancer therapy and many are in clinical trials. Among the 11 zinc-dependent HDACs, HDAC10 has received relatively little attention by drug discovery campaigns, despite its involvement e.g. in the pathogenesis of neuroblastoma. This is due in part to a lack of robust enzymatic conversion assays. In contrast to the protein lysine deacetylase and deacylase activity of the other HDAC subtypes, it has recently been shown that HDAC10 has strong preferences for deacetylation of oligoamine substrates like spermine or spermidine. Hence, it also termed a polyamine deacetylase (PDAC). Here, we present the first fluorescent enzymatic conversion assay for HDAC10 using an aminocoumarin labelled acetyl spermidine derivative to measure its PDAC activity, which is suitable for high-throughput screening. Using this assay, we identified potent inhibitors of HDAC10 mediated spermidine deacetylation in-vitro. Among those are potent inhibitors of neuroblastoma colony growth in culture that show accumulation of lysosomes, implicating disturbance of autophagic flux.

Sign in / Sign up

Export Citation Format

Share Document